Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22655 and RR42_RS22660 are separated by 56 nucleotides RR42_RS22660 and RR42_RS22665 are separated by 176 nucleotides RR42_RS22665 and RR42_RS22670 are separated by 375 nucleotides
RR42_RS22655: RR42_RS22655 - mandelate racemase, at 416,656 to 417,813
_RS22655
RR42_RS22660: RR42_RS22660 - hypothetical protein, at 417,870 to 418,847
_RS22660
RR42_RS22665: RR42_RS22665 - hypothetical protein, at 419,024 to 419,311
_RS22665
RR42_RS22670: RR42_RS22670 - penicillin acylase, at 419,687 to 422,188
_RS22670
Position (kb) 417
418
419 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 417.109 kb on + strand at 417.109 kb on + strand, within RR42_RS22655 at 417.109 kb on + strand, within RR42_RS22655 at 417.109 kb on + strand, within RR42_RS22655 at 417.109 kb on + strand, within RR42_RS22655 at 417.110 kb on - strand, within RR42_RS22655 at 417.136 kb on - strand, within RR42_RS22655 at 417.136 kb on - strand, within RR42_RS22655 at 417.460 kb on - strand, within RR42_RS22655 at 417.856 kb on + strand at 417.856 kb on + strand at 417.893 kb on - strand at 418.137 kb on + strand, within RR42_RS22660 at 418.137 kb on + strand, within RR42_RS22660 at 418.137 kb on + strand, within RR42_RS22660 at 418.173 kb on + strand, within RR42_RS22660 at 418.185 kb on + strand, within RR42_RS22660 at 418.185 kb on + strand, within RR42_RS22660 at 418.186 kb on - strand, within RR42_RS22660 at 418.186 kb on - strand, within RR42_RS22660 at 418.251 kb on + strand, within RR42_RS22660 at 418.251 kb on + strand, within RR42_RS22660 at 418.252 kb on - strand, within RR42_RS22660 at 418.308 kb on + strand, within RR42_RS22660 at 418.309 kb on - strand, within RR42_RS22660 at 418.309 kb on - strand, within RR42_RS22660 at 418.339 kb on - strand, within RR42_RS22660 at 418.339 kb on - strand, within RR42_RS22660 at 418.416 kb on + strand, within RR42_RS22660 at 418.416 kb on + strand, within RR42_RS22660 at 418.416 kb on + strand, within RR42_RS22660 at 418.416 kb on + strand, within RR42_RS22660 at 418.417 kb on - strand, within RR42_RS22660 at 418.425 kb on + strand, within RR42_RS22660 at 418.426 kb on - strand, within RR42_RS22660 at 418.426 kb on - strand, within RR42_RS22660 at 418.510 kb on - strand, within RR42_RS22660 at 418.510 kb on - strand, within RR42_RS22660 at 418.510 kb on - strand, within RR42_RS22660 at 418.510 kb on - strand, within RR42_RS22660 at 418.583 kb on + strand, within RR42_RS22660 at 418.907 kb on + strand at 418.908 kb on - strand at 418.911 kb on - strand at 418.921 kb on + strand at 418.922 kb on - strand at 418.922 kb on - strand at 418.922 kb on - strand at 418.947 kb on - strand at 418.947 kb on - strand at 418.947 kb on - strand at 419.036 kb on + strand at 419.036 kb on + strand at 419.037 kb on - strand at 419.037 kb on - strand at 419.162 kb on + strand, within RR42_RS22665 at 419.162 kb on + strand, within RR42_RS22665 at 419.162 kb on + strand, within RR42_RS22665 at 419.163 kb on - strand, within RR42_RS22665 at 419.163 kb on - strand, within RR42_RS22665 at 419.163 kb on - strand, within RR42_RS22665 at 419.163 kb on - strand, within RR42_RS22665 at 419.163 kb on - strand, within RR42_RS22665 at 419.357 kb on - strand at 419.415 kb on - strand at 419.536 kb on - strand at 419.775 kb on - strand at 419.775 kb on - strand at 419.775 kb on - strand at 419.775 kb on - strand at 419.775 kb on - strand at 419.790 kb on - strand at 419.801 kb on + strand at 419.801 kb on + strand at 419.801 kb on + strand at 419.801 kb on + strand at 419.801 kb on + strand at 419.801 kb on + strand at 419.802 kb on - strand at 419.802 kb on - strand at 419.807 kb on + strand at 419.807 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 417,109 + +3.2 417,109 + RR42_RS22655 0.39 +1.1 417,109 + RR42_RS22655 0.39 +0.1 417,109 + RR42_RS22655 0.39 -0.6 417,109 + RR42_RS22655 0.39 +0.6 417,110 - RR42_RS22655 0.39 -0.9 417,136 - RR42_RS22655 0.41 -0.1 417,136 - RR42_RS22655 0.41 -0.4 417,460 - RR42_RS22655 0.69 -0.9 417,856 + +0.2 417,856 + -0.3 417,893 - -0.5 418,137 + RR42_RS22660 0.27 +0.2 418,137 + RR42_RS22660 0.27 -0.3 418,137 + RR42_RS22660 0.27 +0.3 418,173 + RR42_RS22660 0.31 +0.3 418,185 + RR42_RS22660 0.32 +0.3 418,185 + RR42_RS22660 0.32 +0.4 418,186 - RR42_RS22660 0.32 +0.2 418,186 - RR42_RS22660 0.32 -0.7 418,251 + RR42_RS22660 0.39 +3.6 418,251 + RR42_RS22660 0.39 +0.0 418,252 - RR42_RS22660 0.39 -0.8 418,308 + RR42_RS22660 0.45 -0.5 418,309 - RR42_RS22660 0.45 -0.1 418,309 - RR42_RS22660 0.45 -0.7 418,339 - RR42_RS22660 0.48 +0.3 418,339 - RR42_RS22660 0.48 +0.6 418,416 + RR42_RS22660 0.56 -0.5 418,416 + RR42_RS22660 0.56 +0.7 418,416 + RR42_RS22660 0.56 -0.9 418,416 + RR42_RS22660 0.56 +1.1 418,417 - RR42_RS22660 0.56 +0.6 418,425 + RR42_RS22660 0.57 -0.8 418,426 - RR42_RS22660 0.57 -0.3 418,426 - RR42_RS22660 0.57 +0.9 418,510 - RR42_RS22660 0.65 -0.2 418,510 - RR42_RS22660 0.65 -0.2 418,510 - RR42_RS22660 0.65 +1.5 418,510 - RR42_RS22660 0.65 +0.3 418,583 + RR42_RS22660 0.73 +1.2 418,907 + -0.1 418,908 - -1.8 418,911 - -1.3 418,921 + +0.5 418,922 - -0.2 418,922 - -2.6 418,922 - +0.3 418,947 - +0.6 418,947 - +0.3 418,947 - -0.4 419,036 + +0.2 419,036 + +0.3 419,037 - -1.3 419,037 - +0.1 419,162 + RR42_RS22665 0.48 +0.3 419,162 + RR42_RS22665 0.48 +1.6 419,162 + RR42_RS22665 0.48 +0.1 419,163 - RR42_RS22665 0.48 +0.1 419,163 - RR42_RS22665 0.48 -0.4 419,163 - RR42_RS22665 0.48 -0.0 419,163 - RR42_RS22665 0.48 +0.2 419,163 - RR42_RS22665 0.48 +0.3 419,357 - +0.0 419,415 - +0.1 419,536 - -0.3 419,775 - -1.5 419,775 - -1.3 419,775 - -0.0 419,775 - -0.8 419,775 - -0.3 419,790 - +0.6 419,801 + +0.8 419,801 + -1.3 419,801 + -3.0 419,801 + -0.8 419,801 + -0.1 419,801 + +1.1 419,802 - -0.0 419,802 - -0.0 419,807 + -0.8 419,807 + +0.3
Or see this region's nucleotide sequence