Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22650 and RR42_RS22655 are separated by 75 nucleotides RR42_RS22655 and RR42_RS22660 are separated by 56 nucleotides
RR42_RS22650: RR42_RS22650 - ABC transporter substrate-binding protein, at 415,591 to 416,580
_RS22650
RR42_RS22655: RR42_RS22655 - mandelate racemase, at 416,656 to 417,813
_RS22655
RR42_RS22660: RR42_RS22660 - hypothetical protein, at 417,870 to 418,847
_RS22660
Position (kb) 416
417
418 Strain fitness (log2 ratio) -1
0
1
2
3 at 415.687 kb on + strand at 415.688 kb on - strand at 415.870 kb on + strand, within RR42_RS22650 at 415.871 kb on - strand, within RR42_RS22650 at 415.912 kb on + strand, within RR42_RS22650 at 415.913 kb on - strand, within RR42_RS22650 at 415.921 kb on + strand, within RR42_RS22650 at 415.921 kb on + strand, within RR42_RS22650 at 415.922 kb on - strand, within RR42_RS22650 at 415.922 kb on - strand, within RR42_RS22650 at 415.922 kb on - strand, within RR42_RS22650 at 415.922 kb on - strand, within RR42_RS22650 at 416.065 kb on + strand, within RR42_RS22650 at 416.065 kb on + strand, within RR42_RS22650 at 416.158 kb on + strand, within RR42_RS22650 at 416.158 kb on + strand, within RR42_RS22650 at 416.159 kb on - strand, within RR42_RS22650 at 416.277 kb on - strand, within RR42_RS22650 at 416.302 kb on + strand, within RR42_RS22650 at 416.386 kb on - strand, within RR42_RS22650 at 416.386 kb on - strand, within RR42_RS22650 at 416.531 kb on + strand at 416.579 kb on - strand at 416.725 kb on + strand at 416.854 kb on + strand, within RR42_RS22655 at 416.854 kb on + strand, within RR42_RS22655 at 417.109 kb on + strand at 417.109 kb on + strand, within RR42_RS22655 at 417.109 kb on + strand, within RR42_RS22655 at 417.109 kb on + strand, within RR42_RS22655 at 417.109 kb on + strand, within RR42_RS22655 at 417.110 kb on - strand, within RR42_RS22655 at 417.136 kb on - strand, within RR42_RS22655 at 417.136 kb on - strand, within RR42_RS22655 at 417.460 kb on - strand, within RR42_RS22655 at 417.856 kb on + strand at 417.856 kb on + strand at 417.893 kb on - strand at 418.137 kb on + strand, within RR42_RS22660 at 418.137 kb on + strand, within RR42_RS22660 at 418.137 kb on + strand, within RR42_RS22660 at 418.173 kb on + strand, within RR42_RS22660 at 418.185 kb on + strand, within RR42_RS22660 at 418.185 kb on + strand, within RR42_RS22660 at 418.186 kb on - strand, within RR42_RS22660 at 418.186 kb on - strand, within RR42_RS22660 at 418.251 kb on + strand, within RR42_RS22660 at 418.251 kb on + strand, within RR42_RS22660 at 418.252 kb on - strand, within RR42_RS22660 at 418.308 kb on + strand, within RR42_RS22660 at 418.309 kb on - strand, within RR42_RS22660 at 418.309 kb on - strand, within RR42_RS22660 at 418.339 kb on - strand, within RR42_RS22660 at 418.339 kb on - strand, within RR42_RS22660 at 418.416 kb on + strand, within RR42_RS22660 at 418.416 kb on + strand, within RR42_RS22660 at 418.416 kb on + strand, within RR42_RS22660 at 418.416 kb on + strand, within RR42_RS22660 at 418.417 kb on - strand, within RR42_RS22660 at 418.425 kb on + strand, within RR42_RS22660 at 418.426 kb on - strand, within RR42_RS22660 at 418.426 kb on - strand, within RR42_RS22660 at 418.510 kb on - strand, within RR42_RS22660 at 418.510 kb on - strand, within RR42_RS22660 at 418.510 kb on - strand, within RR42_RS22660 at 418.510 kb on - strand, within RR42_RS22660 at 418.583 kb on + strand, within RR42_RS22660
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 415,687 + -0.1 415,688 - +1.2 415,870 + RR42_RS22650 0.28 +1.0 415,871 - RR42_RS22650 0.28 +0.6 415,912 + RR42_RS22650 0.32 +0.6 415,913 - RR42_RS22650 0.33 +0.7 415,921 + RR42_RS22650 0.33 +0.6 415,921 + RR42_RS22650 0.33 -0.0 415,922 - RR42_RS22650 0.33 +0.2 415,922 - RR42_RS22650 0.33 +0.2 415,922 - RR42_RS22650 0.33 +1.0 415,922 - RR42_RS22650 0.33 -0.1 416,065 + RR42_RS22650 0.48 +2.4 416,065 + RR42_RS22650 0.48 -0.3 416,158 + RR42_RS22650 0.57 +0.7 416,158 + RR42_RS22650 0.57 +0.6 416,159 - RR42_RS22650 0.57 -0.3 416,277 - RR42_RS22650 0.69 +0.1 416,302 + RR42_RS22650 0.72 -0.7 416,386 - RR42_RS22650 0.80 -0.3 416,386 - RR42_RS22650 0.80 +0.5 416,531 + -0.1 416,579 - +1.4 416,725 + -0.6 416,854 + RR42_RS22655 0.17 -0.2 416,854 + RR42_RS22655 0.17 -0.2 417,109 + +3.2 417,109 + RR42_RS22655 0.39 +1.1 417,109 + RR42_RS22655 0.39 +0.1 417,109 + RR42_RS22655 0.39 -0.6 417,109 + RR42_RS22655 0.39 +0.6 417,110 - RR42_RS22655 0.39 -0.9 417,136 - RR42_RS22655 0.41 -0.1 417,136 - RR42_RS22655 0.41 -0.4 417,460 - RR42_RS22655 0.69 -0.9 417,856 + +0.2 417,856 + -0.3 417,893 - -0.5 418,137 + RR42_RS22660 0.27 +0.2 418,137 + RR42_RS22660 0.27 -0.3 418,137 + RR42_RS22660 0.27 +0.3 418,173 + RR42_RS22660 0.31 +0.3 418,185 + RR42_RS22660 0.32 +0.3 418,185 + RR42_RS22660 0.32 +0.4 418,186 - RR42_RS22660 0.32 +0.2 418,186 - RR42_RS22660 0.32 -0.7 418,251 + RR42_RS22660 0.39 +3.6 418,251 + RR42_RS22660 0.39 +0.0 418,252 - RR42_RS22660 0.39 -0.8 418,308 + RR42_RS22660 0.45 -0.5 418,309 - RR42_RS22660 0.45 -0.1 418,309 - RR42_RS22660 0.45 -0.7 418,339 - RR42_RS22660 0.48 +0.3 418,339 - RR42_RS22660 0.48 +0.6 418,416 + RR42_RS22660 0.56 -0.5 418,416 + RR42_RS22660 0.56 +0.7 418,416 + RR42_RS22660 0.56 -0.9 418,416 + RR42_RS22660 0.56 +1.1 418,417 - RR42_RS22660 0.56 +0.6 418,425 + RR42_RS22660 0.57 -0.8 418,426 - RR42_RS22660 0.57 -0.3 418,426 - RR42_RS22660 0.57 +0.9 418,510 - RR42_RS22660 0.65 -0.2 418,510 - RR42_RS22660 0.65 -0.2 418,510 - RR42_RS22660 0.65 +1.5 418,510 - RR42_RS22660 0.65 +0.3 418,583 + RR42_RS22660 0.73 +1.2
Or see this region's nucleotide sequence