Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS11840 and RR42_RS11845 are separated by 138 nucleotides RR42_RS11845 and RR42_RS11850 are separated by 11 nucleotides RR42_RS11850 and RR42_RS11855 are separated by 37 nucleotides
RR42_RS11840: RR42_RS11840 - zinc metalloprotease, at 2,531,635 to 2,533,026
_RS11840
RR42_RS11845: RR42_RS11845 - 1-deoxy-D-xylulose 5-phosphate reductoisomerase, at 2,533,165 to 2,534,343
_RS11845
RR42_RS11850: RR42_RS11850 - phosphatidate cytidylyltransferase, at 2,534,355 to 2,535,176
_RS11850
RR42_RS11855: RR42_RS11855 - UDP diphosphate synthase, at 2,535,214 to 2,535,987
_RS11855
Position (kb)
2533
2534
2535 Strain fitness (log2 ratio)
-1
0
1 at 2532.668 kb on + strand, within RR42_RS11840 at 2532.827 kb on + strand, within RR42_RS11840 at 2532.959 kb on + strand at 2532.959 kb on + strand at 2532.959 kb on + strand at 2532.981 kb on - strand at 2533.015 kb on + strand at 2533.074 kb on + strand at 2533.074 kb on + strand at 2533.131 kb on - strand at 2534.363 kb on - strand at 2534.363 kb on - strand at 2534.363 kb on - strand at 2534.363 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 2,532,668 + RR42_RS11840 0.74 -1.4 2,532,827 + RR42_RS11840 0.86 -1.1 2,532,959 + -1.3 2,532,959 + -1.5 2,532,959 + -0.9 2,532,981 - -0.8 2,533,015 + -1.5 2,533,074 + -0.4 2,533,074 + -0.6 2,533,131 - +0.3 2,534,363 - +0.2 2,534,363 - +0.4 2,534,363 - -0.5 2,534,363 - -1.2
Or see this region's nucleotide sequence