Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS09395 and RR42_RS09400 are separated by 343 nucleotides RR42_RS09400 and RR42_RS09405 are separated by 176 nucleotides
RR42_RS09395: RR42_RS09395 - multidrug transporter MatE, at 1,990,267 to 1,991,580
_RS09395
RR42_RS09400: RR42_RS09400 - membrane protein, at 1,991,924 to 1,993,072
_RS09400
RR42_RS09405: RR42_RS09405 - butanediol dehydrogenase, at 1,993,249 to 1,994,322
_RS09405
Position (kb)
1991
1992
1993
1994 Strain fitness (log2 ratio)
-2
-1
0
1 at 1991.038 kb on + strand, within RR42_RS09395 at 1991.038 kb on + strand, within RR42_RS09395 at 1991.039 kb on - strand, within RR42_RS09395 at 1991.042 kb on + strand, within RR42_RS09395 at 1991.043 kb on - strand, within RR42_RS09395 at 1991.049 kb on - strand, within RR42_RS09395 at 1991.148 kb on - strand, within RR42_RS09395 at 1991.266 kb on + strand, within RR42_RS09395 at 1991.267 kb on - strand, within RR42_RS09395 at 1991.354 kb on + strand, within RR42_RS09395 at 1991.695 kb on - strand at 1991.758 kb on + strand at 1991.759 kb on - strand at 1991.759 kb on - strand at 1991.759 kb on - strand at 1991.806 kb on - strand at 1991.833 kb on - strand at 1991.920 kb on + strand at 1991.949 kb on + strand at 1991.950 kb on - strand at 1991.950 kb on - strand at 1991.970 kb on + strand at 1991.970 kb on + strand at 1991.985 kb on + strand at 1991.985 kb on + strand at 1991.986 kb on - strand at 1992.076 kb on + strand, within RR42_RS09400 at 1992.076 kb on + strand, within RR42_RS09400 at 1992.076 kb on + strand, within RR42_RS09400 at 1992.076 kb on + strand, within RR42_RS09400 at 1992.077 kb on - strand, within RR42_RS09400 at 1992.125 kb on + strand, within RR42_RS09400 at 1992.309 kb on + strand, within RR42_RS09400 at 1992.309 kb on + strand, within RR42_RS09400 at 1992.359 kb on - strand, within RR42_RS09400 at 1992.425 kb on + strand, within RR42_RS09400 at 1992.426 kb on - strand, within RR42_RS09400 at 1992.426 kb on - strand, within RR42_RS09400 at 1992.438 kb on + strand, within RR42_RS09400 at 1992.510 kb on + strand, within RR42_RS09400 at 1992.525 kb on + strand, within RR42_RS09400 at 1992.526 kb on - strand, within RR42_RS09400 at 1992.526 kb on - strand, within RR42_RS09400 at 1992.645 kb on + strand, within RR42_RS09400 at 1992.645 kb on + strand, within RR42_RS09400 at 1992.879 kb on + strand, within RR42_RS09400 at 1993.051 kb on + strand at 1993.089 kb on - strand at 1993.089 kb on - strand at 1993.089 kb on - strand at 1993.430 kb on + strand, within RR42_RS09405 at 1993.430 kb on + strand, within RR42_RS09405 at 1993.431 kb on - strand, within RR42_RS09405 at 1993.466 kb on - strand, within RR42_RS09405 at 1993.605 kb on - strand, within RR42_RS09405 at 1993.901 kb on + strand, within RR42_RS09405 at 1993.902 kb on - strand, within RR42_RS09405 at 1994.009 kb on + strand, within RR42_RS09405
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 1,991,038 + RR42_RS09395 0.59 -0.2 1,991,038 + RR42_RS09395 0.59 -0.1 1,991,039 - RR42_RS09395 0.59 -1.9 1,991,042 + RR42_RS09395 0.59 -1.9 1,991,043 - RR42_RS09395 0.59 -0.2 1,991,049 - RR42_RS09395 0.60 -0.2 1,991,148 - RR42_RS09395 0.67 +1.1 1,991,266 + RR42_RS09395 0.76 -0.6 1,991,267 - RR42_RS09395 0.76 -0.2 1,991,354 + RR42_RS09395 0.83 -0.0 1,991,695 - -0.1 1,991,758 + +0.2 1,991,759 - -0.6 1,991,759 - +0.3 1,991,759 - -0.7 1,991,806 - -0.1 1,991,833 - +1.1 1,991,920 + +0.2 1,991,949 + -0.5 1,991,950 - -0.5 1,991,950 - -1.5 1,991,970 + -0.1 1,991,970 + -2.0 1,991,985 + +0.1 1,991,985 + +0.3 1,991,986 - -0.1 1,992,076 + RR42_RS09400 0.13 -0.2 1,992,076 + RR42_RS09400 0.13 -0.1 1,992,076 + RR42_RS09400 0.13 -0.1 1,992,076 + RR42_RS09400 0.13 -0.5 1,992,077 - RR42_RS09400 0.13 -1.2 1,992,125 + RR42_RS09400 0.17 -0.5 1,992,309 + RR42_RS09400 0.34 -0.0 1,992,309 + RR42_RS09400 0.34 +0.5 1,992,359 - RR42_RS09400 0.38 -0.7 1,992,425 + RR42_RS09400 0.44 +0.4 1,992,426 - RR42_RS09400 0.44 +0.5 1,992,426 - RR42_RS09400 0.44 +0.2 1,992,438 + RR42_RS09400 0.45 -1.2 1,992,510 + RR42_RS09400 0.51 -0.1 1,992,525 + RR42_RS09400 0.52 +1.2 1,992,526 - RR42_RS09400 0.52 -1.1 1,992,526 - RR42_RS09400 0.52 +0.6 1,992,645 + RR42_RS09400 0.63 -0.4 1,992,645 + RR42_RS09400 0.63 -1.8 1,992,879 + RR42_RS09400 0.83 +0.6 1,993,051 + -0.2 1,993,089 - -1.9 1,993,089 - -0.1 1,993,089 - +1.0 1,993,430 + RR42_RS09405 0.17 +0.3 1,993,430 + RR42_RS09405 0.17 +1.0 1,993,431 - RR42_RS09405 0.17 +0.3 1,993,466 - RR42_RS09405 0.20 -1.6 1,993,605 - RR42_RS09405 0.33 -0.9 1,993,901 + RR42_RS09405 0.61 -0.6 1,993,902 - RR42_RS09405 0.61 +0.5 1,994,009 + RR42_RS09405 0.71 -0.2
Or see this region's nucleotide sequence