Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04215 and RR42_RS04220 are separated by 6 nucleotides RR42_RS04220 and RR42_RS04225 are separated by 15 nucleotides RR42_RS04225 and RR42_RS04230 are separated by 426 nucleotides
RR42_RS04215: RR42_RS04215 - hypothetical protein, at 933,296 to 933,799
_RS04215
RR42_RS04220: RR42_RS04220 - membrane protein, at 933,806 to 934,666
_RS04220
RR42_RS04225: RR42_RS04225 - hypothetical protein, at 934,682 to 935,302
_RS04225
RR42_RS04230: RR42_RS04230 - dihydrodipicolinate synthase, at 935,729 to 936,637
_RS04230
Position (kb)
934
935
936 Strain fitness (log2 ratio)
-2
-1
0
1 at 934.185 kb on + strand, within RR42_RS04220 at 934.186 kb on - strand, within RR42_RS04220 at 934.392 kb on + strand, within RR42_RS04220 at 934.392 kb on + strand, within RR42_RS04220 at 934.424 kb on + strand, within RR42_RS04220 at 934.464 kb on + strand, within RR42_RS04220 at 934.610 kb on + strand at 934.666 kb on + strand at 934.667 kb on - strand at 934.667 kb on - strand at 934.667 kb on - strand at 934.667 kb on - strand at 934.670 kb on + strand at 934.670 kb on + strand at 934.670 kb on + strand at 934.670 kb on + strand at 934.671 kb on - strand at 934.671 kb on - strand at 934.671 kb on - strand at 934.671 kb on - strand at 934.671 kb on - strand at 934.671 kb on - strand at 934.806 kb on + strand, within RR42_RS04225 at 934.807 kb on - strand, within RR42_RS04225 at 934.807 kb on - strand, within RR42_RS04225 at 934.826 kb on - strand, within RR42_RS04225 at 934.827 kb on + strand, within RR42_RS04225 at 934.827 kb on + strand, within RR42_RS04225 at 934.828 kb on - strand, within RR42_RS04225 at 934.828 kb on - strand, within RR42_RS04225 at 934.828 kb on - strand, within RR42_RS04225 at 934.828 kb on - strand, within RR42_RS04225 at 935.091 kb on + strand, within RR42_RS04225 at 935.092 kb on - strand, within RR42_RS04225 at 935.092 kb on - strand, within RR42_RS04225 at 935.105 kb on - strand, within RR42_RS04225 at 935.143 kb on - strand, within RR42_RS04225 at 935.205 kb on + strand, within RR42_RS04225 at 935.205 kb on + strand, within RR42_RS04225 at 935.258 kb on + strand at 935.258 kb on + strand at 935.335 kb on + strand at 935.336 kb on - strand at 935.336 kb on - strand at 935.362 kb on + strand at 935.363 kb on - strand at 935.613 kb on + strand at 935.613 kb on + strand at 935.614 kb on - strand at 935.614 kb on - strand at 935.614 kb on - strand at 935.714 kb on + strand at 935.714 kb on + strand at 935.739 kb on + strand at 935.740 kb on - strand at 935.748 kb on + strand at 935.748 kb on + strand at 935.748 kb on + strand at 935.748 kb on + strand at 935.749 kb on - strand at 935.749 kb on - strand at 935.843 kb on + strand, within RR42_RS04230 at 936.068 kb on + strand, within RR42_RS04230 at 936.068 kb on + strand, within RR42_RS04230 at 936.068 kb on + strand, within RR42_RS04230 at 936.147 kb on - strand, within RR42_RS04230 at 936.147 kb on - strand, within RR42_RS04230 at 936.147 kb on - strand, within RR42_RS04230 at 936.248 kb on + strand, within RR42_RS04230 at 936.249 kb on - strand, within RR42_RS04230 at 936.249 kb on - strand, within RR42_RS04230
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 934,185 + RR42_RS04220 0.44 -0.0 934,186 - RR42_RS04220 0.44 -0.3 934,392 + RR42_RS04220 0.68 -0.3 934,392 + RR42_RS04220 0.68 -0.1 934,424 + RR42_RS04220 0.72 -2.0 934,464 + RR42_RS04220 0.76 -0.6 934,610 + +0.1 934,666 + +0.3 934,667 - -0.1 934,667 - -0.3 934,667 - +0.3 934,667 - -0.4 934,670 + +0.3 934,670 + +0.0 934,670 + +0.0 934,670 + -0.3 934,671 - -0.1 934,671 - +0.3 934,671 - -0.6 934,671 - -0.9 934,671 - -0.3 934,671 - -0.1 934,806 + RR42_RS04225 0.20 -0.1 934,807 - RR42_RS04225 0.20 -1.5 934,807 - RR42_RS04225 0.20 +0.1 934,826 - RR42_RS04225 0.23 +0.0 934,827 + RR42_RS04225 0.23 -0.1 934,827 + RR42_RS04225 0.23 -0.1 934,828 - RR42_RS04225 0.24 -0.4 934,828 - RR42_RS04225 0.24 +0.1 934,828 - RR42_RS04225 0.24 -0.3 934,828 - RR42_RS04225 0.24 -1.4 935,091 + RR42_RS04225 0.66 +0.2 935,092 - RR42_RS04225 0.66 -0.0 935,092 - RR42_RS04225 0.66 -0.2 935,105 - RR42_RS04225 0.68 -0.6 935,143 - RR42_RS04225 0.74 +0.5 935,205 + RR42_RS04225 0.84 -1.5 935,205 + RR42_RS04225 0.84 +0.2 935,258 + +0.5 935,258 + -0.1 935,335 + -0.1 935,336 - -0.7 935,336 - +0.3 935,362 + -0.6 935,363 - +0.4 935,613 + +0.7 935,613 + -0.9 935,614 - -0.5 935,614 - -0.0 935,614 - -0.5 935,714 + -1.0 935,714 + +0.6 935,739 + +0.7 935,740 - -0.4 935,748 + +1.4 935,748 + +0.1 935,748 + -0.3 935,748 + +0.4 935,749 - +1.1 935,749 - -2.1 935,843 + RR42_RS04230 0.13 +0.4 936,068 + RR42_RS04230 0.37 +1.3 936,068 + RR42_RS04230 0.37 +0.8 936,068 + RR42_RS04230 0.37 -0.1 936,147 - RR42_RS04230 0.46 +0.1 936,147 - RR42_RS04230 0.46 +0.1 936,147 - RR42_RS04230 0.46 +0.8 936,248 + RR42_RS04230 0.57 -0.9 936,249 - RR42_RS04230 0.57 -0.2 936,249 - RR42_RS04230 0.57 +0.1
Or see this region's nucleotide sequence