Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03270 and RR42_RS03275 are separated by 94 nucleotides RR42_RS03275 and RR42_RS03280 are separated by 117 nucleotides RR42_RS03280 and RR42_RS03285 are separated by 57 nucleotides
RR42_RS03270: RR42_RS03270 - membrane protein, at 724,868 to 725,077
_RS03270
RR42_RS03275: RR42_RS03275 - membrane protein, at 725,172 to 725,642
_RS03275
RR42_RS03280: RR42_RS03280 - LysR family transcriptional regulator, at 725,760 to 726,665
_RS03280
RR42_RS03285: RR42_RS03285 - tricarboxylic transport membrane protein, at 726,723 to 727,679
_RS03285
Position (kb)
725
726
727 Strain fitness (log2 ratio)
-2
-1
0
1 at 724.764 kb on + strand at 724.764 kb on + strand at 724.765 kb on - strand at 724.765 kb on - strand at 724.765 kb on - strand at 724.765 kb on - strand at 724.874 kb on - strand at 724.879 kb on - strand at 724.888 kb on + strand at 724.888 kb on + strand at 725.166 kb on + strand at 725.166 kb on + strand at 725.167 kb on - strand at 725.167 kb on - strand at 725.167 kb on - strand at 725.167 kb on - strand at 725.187 kb on + strand at 725.281 kb on + strand, within RR42_RS03275 at 725.290 kb on + strand, within RR42_RS03275 at 725.290 kb on + strand, within RR42_RS03275 at 725.290 kb on + strand, within RR42_RS03275 at 725.290 kb on + strand, within RR42_RS03275 at 725.290 kb on + strand, within RR42_RS03275 at 725.290 kb on + strand, within RR42_RS03275 at 725.291 kb on - strand, within RR42_RS03275 at 725.291 kb on - strand, within RR42_RS03275 at 725.291 kb on - strand, within RR42_RS03275 at 725.479 kb on + strand, within RR42_RS03275 at 725.480 kb on - strand, within RR42_RS03275 at 725.612 kb on + strand at 725.613 kb on - strand at 725.667 kb on + strand at 725.667 kb on + strand at 725.667 kb on + strand at 725.667 kb on + strand at 725.668 kb on - strand at 725.700 kb on - strand at 726.067 kb on - strand, within RR42_RS03280 at 726.194 kb on - strand, within RR42_RS03280 at 726.194 kb on - strand, within RR42_RS03280 at 726.194 kb on - strand, within RR42_RS03280 at 726.194 kb on - strand, within RR42_RS03280 at 726.663 kb on + strand at 726.664 kb on - strand at 726.698 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.784 kb on + strand at 726.785 kb on - strand at 726.785 kb on - strand at 726.785 kb on - strand at 726.785 kb on - strand at 726.785 kb on - strand at 726.785 kb on - strand at 726.785 kb on - strand at 726.785 kb on - strand at 726.785 kb on - strand at 726.785 kb on - strand at 726.788 kb on + strand at 726.788 kb on + strand at 726.898 kb on + strand, within RR42_RS03285 at 726.899 kb on - strand, within RR42_RS03285 at 726.899 kb on - strand, within RR42_RS03285 at 726.928 kb on + strand, within RR42_RS03285 at 726.929 kb on - strand, within RR42_RS03285 at 726.929 kb on - strand, within RR42_RS03285 at 727.165 kb on + strand, within RR42_RS03285 at 727.165 kb on + strand, within RR42_RS03285 at 727.166 kb on - strand, within RR42_RS03285 at 727.245 kb on + strand, within RR42_RS03285 at 727.245 kb on + strand, within RR42_RS03285 at 727.245 kb on + strand, within RR42_RS03285 at 727.245 kb on + strand, within RR42_RS03285 at 727.246 kb on - strand, within RR42_RS03285 at 727.246 kb on - strand, within RR42_RS03285 at 727.246 kb on - strand, within RR42_RS03285 at 727.320 kb on + strand, within RR42_RS03285 at 727.331 kb on - strand, within RR42_RS03285 at 727.375 kb on + strand, within RR42_RS03285 at 727.376 kb on - strand, within RR42_RS03285 at 727.376 kb on - strand, within RR42_RS03285 at 727.474 kb on + strand, within RR42_RS03285 at 727.474 kb on + strand, within RR42_RS03285 at 727.474 kb on + strand, within RR42_RS03285 at 727.474 kb on + strand, within RR42_RS03285 at 727.475 kb on - strand, within RR42_RS03285 at 727.505 kb on - strand, within RR42_RS03285
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 724,764 + +1.4 724,764 + -1.2 724,765 - -0.1 724,765 - +0.6 724,765 - -0.3 724,765 - +0.3 724,874 - +0.3 724,879 - +0.1 724,888 + -0.6 724,888 + -0.3 725,166 + -1.1 725,166 + -0.1 725,167 - +0.5 725,167 - -0.1 725,167 - +1.2 725,167 - -0.2 725,187 + +1.2 725,281 + RR42_RS03275 0.23 +0.7 725,290 + RR42_RS03275 0.25 +0.2 725,290 + RR42_RS03275 0.25 +0.4 725,290 + RR42_RS03275 0.25 +0.2 725,290 + RR42_RS03275 0.25 +0.4 725,290 + RR42_RS03275 0.25 +1.4 725,290 + RR42_RS03275 0.25 +1.1 725,291 - RR42_RS03275 0.25 -0.3 725,291 - RR42_RS03275 0.25 +0.2 725,291 - RR42_RS03275 0.25 -0.2 725,479 + RR42_RS03275 0.65 -0.7 725,480 - RR42_RS03275 0.65 +0.1 725,612 + +0.3 725,613 - -1.6 725,667 + +0.4 725,667 + +0.3 725,667 + +0.1 725,667 + -0.3 725,668 - +1.0 725,700 - -0.1 726,067 - RR42_RS03280 0.34 +0.4 726,194 - RR42_RS03280 0.48 +0.7 726,194 - RR42_RS03280 0.48 +0.4 726,194 - RR42_RS03280 0.48 -0.2 726,194 - RR42_RS03280 0.48 -0.1 726,663 + -0.0 726,664 - -0.2 726,698 + +0.8 726,784 + -0.3 726,784 + -0.1 726,784 + -0.1 726,784 + -0.9 726,784 + +1.1 726,784 + +1.6 726,784 + +0.1 726,784 + +0.1 726,784 + +0.0 726,784 + +0.2 726,784 + -0.7 726,785 - +1.0 726,785 - +0.6 726,785 - +0.5 726,785 - -0.9 726,785 - +1.1 726,785 - +0.2 726,785 - +0.5 726,785 - +0.2 726,785 - -0.2 726,785 - +1.2 726,788 + -0.2 726,788 + +0.1 726,898 + RR42_RS03285 0.18 -2.0 726,899 - RR42_RS03285 0.18 +0.6 726,899 - RR42_RS03285 0.18 -0.2 726,928 + RR42_RS03285 0.21 +0.6 726,929 - RR42_RS03285 0.22 +0.8 726,929 - RR42_RS03285 0.22 -0.4 727,165 + RR42_RS03285 0.46 +0.1 727,165 + RR42_RS03285 0.46 +0.6 727,166 - RR42_RS03285 0.46 -1.1 727,245 + RR42_RS03285 0.55 +0.5 727,245 + RR42_RS03285 0.55 +1.0 727,245 + RR42_RS03285 0.55 +0.6 727,245 + RR42_RS03285 0.55 +0.2 727,246 - RR42_RS03285 0.55 -0.3 727,246 - RR42_RS03285 0.55 +0.3 727,246 - RR42_RS03285 0.55 -0.1 727,320 + RR42_RS03285 0.62 -0.9 727,331 - RR42_RS03285 0.64 +0.1 727,375 + RR42_RS03285 0.68 +0.4 727,376 - RR42_RS03285 0.68 +1.7 727,376 - RR42_RS03285 0.68 -0.6 727,474 + RR42_RS03285 0.78 +0.5 727,474 + RR42_RS03285 0.78 -1.2 727,474 + RR42_RS03285 0.78 -0.1 727,474 + RR42_RS03285 0.78 +0.7 727,475 - RR42_RS03285 0.79 +0.5 727,505 - RR42_RS03285 0.82 +0.1
Or see this region's nucleotide sequence