Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03045 and RR42_RS03050 are separated by 106 nucleotides RR42_RS03050 and RR42_RS03055 are separated by 300 nucleotides RR42_RS03055 and RR42_RS03060 are separated by 204 nucleotides
RR42_RS03045: RR42_RS03045 - dioxygenase, at 674,200 to 675,021
_RS03045
RR42_RS03050: RR42_RS03050 - energy transducer TonB, at 675,128 to 675,868
_RS03050
RR42_RS03055: RR42_RS03055 - tRNA-Met, at 676,169 to 676,245
_RS03055
RR42_RS03060: RR42_RS03060 - MFS transporter, at 676,450 to 678,150
_RS03060
Position (kb) 675
676 Strain fitness (log2 ratio) -2
-1
0
1
2 at 674.216 kb on - strand at 674.407 kb on + strand, within RR42_RS03045 at 674.408 kb on - strand, within RR42_RS03045 at 674.408 kb on - strand, within RR42_RS03045 at 674.408 kb on - strand, within RR42_RS03045 at 674.473 kb on + strand, within RR42_RS03045 at 674.474 kb on - strand, within RR42_RS03045 at 674.530 kb on + strand, within RR42_RS03045 at 674.530 kb on + strand, within RR42_RS03045 at 674.530 kb on + strand, within RR42_RS03045 at 674.530 kb on + strand, within RR42_RS03045 at 674.530 kb on + strand, within RR42_RS03045 at 674.531 kb on - strand, within RR42_RS03045 at 674.531 kb on - strand, within RR42_RS03045 at 674.531 kb on - strand, within RR42_RS03045 at 674.531 kb on - strand, within RR42_RS03045 at 674.531 kb on - strand, within RR42_RS03045 at 674.531 kb on - strand, within RR42_RS03045 at 674.531 kb on - strand, within RR42_RS03045 at 674.531 kb on - strand, within RR42_RS03045 at 674.532 kb on + strand, within RR42_RS03045 at 674.532 kb on + strand, within RR42_RS03045 at 674.532 kb on + strand, within RR42_RS03045 at 674.532 kb on + strand, within RR42_RS03045 at 674.532 kb on + strand, within RR42_RS03045 at 674.533 kb on - strand, within RR42_RS03045 at 674.552 kb on - strand, within RR42_RS03045 at 674.552 kb on - strand, within RR42_RS03045 at 674.819 kb on - strand, within RR42_RS03045 at 674.969 kb on + strand at 675.762 kb on + strand, within RR42_RS03050 at 675.762 kb on + strand, within RR42_RS03050 at 675.766 kb on - strand, within RR42_RS03050 at 675.766 kb on - strand, within RR42_RS03050 at 675.766 kb on - strand, within RR42_RS03050 at 675.846 kb on + strand at 675.847 kb on - strand at 675.847 kb on - strand at 675.900 kb on + strand at 675.900 kb on + strand at 675.900 kb on + strand at 675.900 kb on + strand at 675.900 kb on + strand at 675.901 kb on - strand at 675.901 kb on - strand at 675.901 kb on - strand at 675.901 kb on - strand at 676.004 kb on + strand at 676.004 kb on + strand at 676.004 kb on + strand at 676.004 kb on + strand at 676.005 kb on - strand at 676.167 kb on + strand at 676.168 kb on - strand at 676.463 kb on + strand at 676.463 kb on + strand at 676.463 kb on + strand at 676.464 kb on - strand at 676.464 kb on - strand at 676.550 kb on + strand at 676.551 kb on - strand at 676.553 kb on + strand at 676.553 kb on + strand at 676.631 kb on + strand, within RR42_RS03060 at 676.632 kb on - strand, within RR42_RS03060 at 676.632 kb on - strand, within RR42_RS03060 at 676.653 kb on - strand, within RR42_RS03060 at 676.653 kb on - strand, within RR42_RS03060 at 676.703 kb on + strand, within RR42_RS03060 at 676.704 kb on - strand, within RR42_RS03060 at 676.718 kb on + strand, within RR42_RS03060 at 676.719 kb on - strand, within RR42_RS03060
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 674,216 - -0.6 674,407 + RR42_RS03045 0.25 +1.4 674,408 - RR42_RS03045 0.25 -0.3 674,408 - RR42_RS03045 0.25 -0.2 674,408 - RR42_RS03045 0.25 -0.1 674,473 + RR42_RS03045 0.33 +0.2 674,474 - RR42_RS03045 0.33 +0.1 674,530 + RR42_RS03045 0.40 +0.4 674,530 + RR42_RS03045 0.40 -1.4 674,530 + RR42_RS03045 0.40 +0.9 674,530 + RR42_RS03045 0.40 +0.2 674,530 + RR42_RS03045 0.40 -1.1 674,531 - RR42_RS03045 0.40 +0.2 674,531 - RR42_RS03045 0.40 -0.1 674,531 - RR42_RS03045 0.40 +0.6 674,531 - RR42_RS03045 0.40 -0.2 674,531 - RR42_RS03045 0.40 -1.2 674,531 - RR42_RS03045 0.40 +0.6 674,531 - RR42_RS03045 0.40 -0.1 674,531 - RR42_RS03045 0.40 -1.6 674,532 + RR42_RS03045 0.40 -0.8 674,532 + RR42_RS03045 0.40 -0.1 674,532 + RR42_RS03045 0.40 +0.3 674,532 + RR42_RS03045 0.40 +1.1 674,532 + RR42_RS03045 0.40 +0.7 674,533 - RR42_RS03045 0.41 +0.3 674,552 - RR42_RS03045 0.43 -0.4 674,552 - RR42_RS03045 0.43 +0.3 674,819 - RR42_RS03045 0.75 +0.0 674,969 + -2.1 675,762 + RR42_RS03050 0.86 -0.0 675,762 + RR42_RS03050 0.86 +0.4 675,766 - RR42_RS03050 0.86 -1.3 675,766 - RR42_RS03050 0.86 +2.7 675,766 - RR42_RS03050 0.86 +1.7 675,846 + -0.1 675,847 - -0.4 675,847 - +1.1 675,900 + +0.5 675,900 + +2.4 675,900 + +0.5 675,900 + -1.1 675,900 + +0.2 675,901 - -0.1 675,901 - -1.0 675,901 - -0.2 675,901 - -0.2 676,004 + -0.4 676,004 + +0.6 676,004 + -1.0 676,004 + +0.4 676,005 - -1.0 676,167 + +0.0 676,168 - -1.3 676,463 + -0.1 676,463 + +0.1 676,463 + -0.7 676,464 - -1.2 676,464 - -0.2 676,550 + +0.5 676,551 - +0.2 676,553 + -0.1 676,553 + -0.1 676,631 + RR42_RS03060 0.11 -1.1 676,632 - RR42_RS03060 0.11 +0.5 676,632 - RR42_RS03060 0.11 +0.8 676,653 - RR42_RS03060 0.12 +1.2 676,653 - RR42_RS03060 0.12 -0.4 676,703 + RR42_RS03060 0.15 -0.9 676,704 - RR42_RS03060 0.15 -0.2 676,718 + RR42_RS03060 0.16 +0.5 676,719 - RR42_RS03060 0.16 -1.1
Or see this region's nucleotide sequence