Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01190 and RR42_RS01195 are separated by 75 nucleotides RR42_RS01195 and RR42_RS01200 are separated by 64 nucleotides RR42_RS01200 and RR42_RS01205 are separated by 29 nucleotides
RR42_RS01190: RR42_RS01190 - peptidase M48, at 272,775 to 273,722
_RS01190
RR42_RS01195: RR42_RS01195 - excinuclease ATPase, at 273,798 to 274,271
_RS01195
RR42_RS01200: RR42_RS01200 - hypothetical protein, at 274,336 to 275,145
_RS01200
RR42_RS01205: RR42_RS01205 - transposase, at 275,175 to 275,579
_RS01205
Position (kb) 273
274
275 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 272.958 kb on + strand, within RR42_RS01190 at 273.168 kb on + strand, within RR42_RS01190 at 273.168 kb on + strand, within RR42_RS01190 at 273.168 kb on + strand, within RR42_RS01190 at 273.168 kb on + strand, within RR42_RS01190 at 273.169 kb on - strand, within RR42_RS01190 at 273.344 kb on + strand, within RR42_RS01190 at 273.345 kb on - strand, within RR42_RS01190 at 273.345 kb on - strand, within RR42_RS01190 at 273.345 kb on - strand at 273.345 kb on - strand, within RR42_RS01190 at 273.447 kb on + strand, within RR42_RS01190 at 273.653 kb on + strand at 273.713 kb on - strand at 273.720 kb on + strand at 273.759 kb on - strand at 273.759 kb on - strand at 273.763 kb on + strand at 273.763 kb on + strand at 273.764 kb on - strand at 273.764 kb on - strand at 273.767 kb on + strand at 273.767 kb on + strand at 274.200 kb on + strand, within RR42_RS01195 at 274.200 kb on + strand, within RR42_RS01195 at 274.201 kb on - strand, within RR42_RS01195 at 274.224 kb on + strand at 274.305 kb on - strand at 274.305 kb on - strand at 274.305 kb on - strand at 274.305 kb on - strand at 274.305 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 272,958 + RR42_RS01190 0.19 -2.1 273,168 + RR42_RS01190 0.41 +0.1 273,168 + RR42_RS01190 0.41 +0.2 273,168 + RR42_RS01190 0.41 +0.6 273,168 + RR42_RS01190 0.41 +0.1 273,169 - RR42_RS01190 0.42 -0.1 273,344 + RR42_RS01190 0.60 +1.2 273,345 - RR42_RS01190 0.60 -0.1 273,345 - RR42_RS01190 0.60 +0.1 273,345 - +1.9 273,345 - RR42_RS01190 0.60 +0.3 273,447 + RR42_RS01190 0.71 +1.8 273,653 + -0.6 273,713 - +0.3 273,720 + -0.1 273,759 - +0.6 273,759 - -1.1 273,763 + +0.1 273,763 + -0.1 273,764 - -0.3 273,764 - +0.3 273,767 + +0.1 273,767 + +0.3 274,200 + RR42_RS01195 0.85 -3.1 274,200 + RR42_RS01195 0.85 -0.7 274,201 - RR42_RS01195 0.85 +0.2 274,224 + +0.1 274,305 - +0.2 274,305 - +0.1 274,305 - +0.4 274,305 - -0.4 274,305 - +0.2
Or see this region's nucleotide sequence