Experiment: R2A with nitrate 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00635 and RR42_RS00640 are separated by 677 nucleotides RR42_RS00640 and RR42_RS00645 overlap by 4 nucleotides
RR42_RS00635: RR42_RS00635 - lipoyl synthase, at 164,909 to 165,904
_RS00635
RR42_RS00640: RR42_RS00640 - allophanate hydrolase, at 166,582 to 167,232
_RS00640
RR42_RS00645: RR42_RS00645 - allophanate hydrolase, at 167,229 to 168,251
_RS00645
Position (kb) 166
167
168 Strain fitness (log2 ratio) -2
-1
0
1
2 at 165.991 kb on + strand at 165.991 kb on + strand at 165.991 kb on + strand at 165.992 kb on - strand at 165.992 kb on - strand at 165.992 kb on - strand at 165.995 kb on + strand at 165.996 kb on - strand at 165.996 kb on - strand at 165.996 kb on - strand at 166.043 kb on + strand at 166.043 kb on + strand at 166.044 kb on - strand at 166.044 kb on - strand at 166.397 kb on + strand at 166.411 kb on + strand at 166.412 kb on - strand at 166.424 kb on + strand at 166.424 kb on + strand at 166.424 kb on + strand at 166.424 kb on + strand at 166.449 kb on + strand at 166.467 kb on - strand at 166.528 kb on + strand at 166.529 kb on - strand at 166.529 kb on - strand at 166.546 kb on + strand at 166.546 kb on + strand at 166.546 kb on + strand at 166.546 kb on + strand at 166.546 kb on + strand at 166.653 kb on - strand, within RR42_RS00640 at 166.653 kb on - strand, within RR42_RS00640 at 166.653 kb on - strand, within RR42_RS00640 at 166.658 kb on + strand, within RR42_RS00640 at 166.658 kb on + strand, within RR42_RS00640 at 166.658 kb on + strand, within RR42_RS00640 at 166.659 kb on - strand, within RR42_RS00640 at 166.659 kb on - strand, within RR42_RS00640 at 166.659 kb on - strand, within RR42_RS00640 at 166.659 kb on - strand, within RR42_RS00640 at 166.659 kb on - strand, within RR42_RS00640 at 166.659 kb on - strand, within RR42_RS00640 at 166.668 kb on + strand, within RR42_RS00640 at 166.668 kb on + strand, within RR42_RS00640 at 166.668 kb on + strand, within RR42_RS00640 at 166.669 kb on - strand, within RR42_RS00640 at 166.951 kb on + strand, within RR42_RS00640 at 166.960 kb on + strand, within RR42_RS00640 at 166.960 kb on + strand, within RR42_RS00640 at 166.961 kb on - strand, within RR42_RS00640 at 166.961 kb on - strand, within RR42_RS00640 at 166.990 kb on + strand, within RR42_RS00640 at 166.990 kb on + strand, within RR42_RS00640 at 166.990 kb on + strand, within RR42_RS00640 at 166.990 kb on + strand, within RR42_RS00640 at 166.991 kb on - strand, within RR42_RS00640 at 166.991 kb on - strand, within RR42_RS00640 at 166.991 kb on - strand, within RR42_RS00640 at 166.992 kb on + strand, within RR42_RS00640 at 166.992 kb on + strand, within RR42_RS00640 at 166.993 kb on - strand, within RR42_RS00640 at 167.095 kb on + strand, within RR42_RS00640 at 167.562 kb on + strand, within RR42_RS00645 at 167.562 kb on + strand, within RR42_RS00645 at 167.562 kb on + strand, within RR42_RS00645 at 167.562 kb on + strand, within RR42_RS00645 at 167.563 kb on - strand, within RR42_RS00645 at 168.157 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with nitrate 200 mM remove 165,991 + +0.2 165,991 + -0.3 165,991 + +0.6 165,992 - +0.1 165,992 - -0.0 165,992 - -0.1 165,995 + -0.5 165,996 - -0.4 165,996 - -0.5 165,996 - -0.5 166,043 + -1.1 166,043 + -0.1 166,044 - -0.6 166,044 - +0.1 166,397 + -1.0 166,411 + -1.2 166,412 - +0.5 166,424 + +0.1 166,424 + -0.0 166,424 + -0.3 166,424 + +0.9 166,449 + +2.1 166,467 - -0.0 166,528 + +0.9 166,529 - -1.2 166,529 - +0.3 166,546 + -0.5 166,546 + +0.3 166,546 + +0.6 166,546 + +0.6 166,546 + +0.1 166,653 - RR42_RS00640 0.11 +0.1 166,653 - RR42_RS00640 0.11 -2.3 166,653 - RR42_RS00640 0.11 +0.9 166,658 + RR42_RS00640 0.12 +0.1 166,658 + RR42_RS00640 0.12 +0.6 166,658 + RR42_RS00640 0.12 +1.7 166,659 - RR42_RS00640 0.12 +0.7 166,659 - RR42_RS00640 0.12 -0.6 166,659 - RR42_RS00640 0.12 +2.5 166,659 - RR42_RS00640 0.12 -0.3 166,659 - RR42_RS00640 0.12 +1.4 166,659 - RR42_RS00640 0.12 -0.2 166,668 + RR42_RS00640 0.13 +0.5 166,668 + RR42_RS00640 0.13 +0.6 166,668 + RR42_RS00640 0.13 +0.6 166,669 - RR42_RS00640 0.13 +1.1 166,951 + RR42_RS00640 0.57 +0.2 166,960 + RR42_RS00640 0.58 +0.2 166,960 + RR42_RS00640 0.58 -0.2 166,961 - RR42_RS00640 0.58 +0.1 166,961 - RR42_RS00640 0.58 +0.2 166,990 + RR42_RS00640 0.63 +0.4 166,990 + RR42_RS00640 0.63 +0.0 166,990 + RR42_RS00640 0.63 -0.4 166,990 + RR42_RS00640 0.63 +0.4 166,991 - RR42_RS00640 0.63 +0.5 166,991 - RR42_RS00640 0.63 +0.0 166,991 - RR42_RS00640 0.63 -0.2 166,992 + RR42_RS00640 0.63 +0.4 166,992 + RR42_RS00640 0.63 +0.4 166,993 - RR42_RS00640 0.63 -1.7 167,095 + RR42_RS00640 0.79 -0.4 167,562 + RR42_RS00645 0.33 +0.1 167,562 + RR42_RS00645 0.33 -0.5 167,562 + RR42_RS00645 0.33 +0.9 167,562 + RR42_RS00645 0.33 +0.4 167,563 - RR42_RS00645 0.33 -0.1 168,157 - -0.5
Or see this region's nucleotide sequence