Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_776

Experiment: L-Asparagine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_775 and Pf1N1B4_776 overlap by 4 nucleotidesPf1N1B4_776 and Pf1N1B4_777 overlap by 1 nucleotidesPf1N1B4_777 and Pf1N1B4_778 are separated by 118 nucleotides Pf1N1B4_775 - Signal transduction histidine kinase regulating C4-dicarboxylate transport system (EC 2.7.13.3), at 879,089 to 880,990 _775 Pf1N1B4_776 - Two component, Sigma-54 Specific, central transcriptional regulator of acidic amino acid uptake, at 880,987 to 882,315 _776 Pf1N1B4_777 - Membrane protein glpM, at 882,315 to 882,653 _777 Pf1N1B4_778 - hypothetical protein, at 882,772 to 883,662 _778 Position (kb) 880 881 882 883Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 880.623 kb on + strand, within Pf1N1B4_775at 880.624 kb on - strand, within Pf1N1B4_775at 880.624 kb on - strand, within Pf1N1B4_775at 880.624 kb on - strand, within Pf1N1B4_775at 880.752 kb on - strand, within Pf1N1B4_775at 880.954 kb on - strandat 880.986 kb on + strandat 881.001 kb on + strandat 881.001 kb on + strandat 881.002 kb on - strandat 881.002 kb on - strandat 881.002 kb on - strandat 881.284 kb on + strand, within Pf1N1B4_776at 881.382 kb on - strand, within Pf1N1B4_776at 881.782 kb on - strand, within Pf1N1B4_776at 881.782 kb on - strand, within Pf1N1B4_776at 881.896 kb on + strand, within Pf1N1B4_776at 881.897 kb on - strand, within Pf1N1B4_776at 881.897 kb on - strand, within Pf1N1B4_776at 881.897 kb on - strand, within Pf1N1B4_776at 881.897 kb on - strand, within Pf1N1B4_776at 881.897 kb on - strand, within Pf1N1B4_776at 881.915 kb on + strand, within Pf1N1B4_776at 882.298 kb on + strandat 882.299 kb on - strandat 882.355 kb on + strand, within Pf1N1B4_777at 882.390 kb on + strand, within Pf1N1B4_777at 882.391 kb on - strand, within Pf1N1B4_777at 882.391 kb on - strand, within Pf1N1B4_777at 882.391 kb on - strand, within Pf1N1B4_777at 882.393 kb on + strand, within Pf1N1B4_777at 882.393 kb on + strand, within Pf1N1B4_777at 882.444 kb on + strand, within Pf1N1B4_777at 882.444 kb on + strand, within Pf1N1B4_777at 882.444 kb on + strand, within Pf1N1B4_777at 882.444 kb on + strand, within Pf1N1B4_777at 882.444 kb on + strand, within Pf1N1B4_777at 882.444 kb on + strand, within Pf1N1B4_777at 882.444 kb on + strand, within Pf1N1B4_777at 882.444 kb on + strand, within Pf1N1B4_777at 882.444 kb on + strand, within Pf1N1B4_777at 882.444 kb on + strand, within Pf1N1B4_777at 882.444 kb on + strand, within Pf1N1B4_777at 882.445 kb on - strand, within Pf1N1B4_777at 882.445 kb on - strand, within Pf1N1B4_777at 882.445 kb on - strand, within Pf1N1B4_777at 882.445 kb on - strand, within Pf1N1B4_777at 882.445 kb on - strand, within Pf1N1B4_777at 882.519 kb on + strand, within Pf1N1B4_777at 882.519 kb on + strand, within Pf1N1B4_777at 882.519 kb on + strand, within Pf1N1B4_777at 882.519 kb on + strand, within Pf1N1B4_777at 882.519 kb on + strand, within Pf1N1B4_777at 882.529 kb on - strand, within Pf1N1B4_777at 882.543 kb on + strand, within Pf1N1B4_777at 882.543 kb on + strand, within Pf1N1B4_777at 882.543 kb on + strand, within Pf1N1B4_777at 882.543 kb on + strand, within Pf1N1B4_777at 882.543 kb on + strand, within Pf1N1B4_777at 882.543 kb on + strand, within Pf1N1B4_777at 882.543 kb on + strand, within Pf1N1B4_777at 882.543 kb on + strand, within Pf1N1B4_777at 882.543 kb on + strand, within Pf1N1B4_777at 882.544 kb on - strand, within Pf1N1B4_777at 882.544 kb on - strand, within Pf1N1B4_777at 882.544 kb on - strand, within Pf1N1B4_777at 882.544 kb on - strandat 882.544 kb on - strand, within Pf1N1B4_777at 882.544 kb on - strand, within Pf1N1B4_777at 882.544 kb on - strand, within Pf1N1B4_777at 882.544 kb on - strand, within Pf1N1B4_777at 882.544 kb on - strand, within Pf1N1B4_777at 882.544 kb on - strand, within Pf1N1B4_777at 882.745 kb on - strandat 882.752 kb on + strandat 882.752 kb on + strandat 882.753 kb on - strandat 882.872 kb on + strand, within Pf1N1B4_778at 882.872 kb on + strand, within Pf1N1B4_778at 882.872 kb on + strand, within Pf1N1B4_778at 882.872 kb on + strand, within Pf1N1B4_778at 882.873 kb on - strandat 882.873 kb on - strand, within Pf1N1B4_778at 882.873 kb on - strand, within Pf1N1B4_778at 882.873 kb on - strand, within Pf1N1B4_778at 882.873 kb on - strand, within Pf1N1B4_778at 882.873 kb on - strand, within Pf1N1B4_778at 882.873 kb on - strand, within Pf1N1B4_778at 882.876 kb on + strand, within Pf1N1B4_778at 882.932 kb on + strand, within Pf1N1B4_778at 882.932 kb on + strand, within Pf1N1B4_778at 882.932 kb on + strand, within Pf1N1B4_778at 882.932 kb on + strand, within Pf1N1B4_778at 882.932 kb on + strand, within Pf1N1B4_778at 882.933 kb on - strand, within Pf1N1B4_778at 883.210 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Asparagine (C)
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880,623 + Pf1N1B4_775 0.81 +0.6
880,624 - Pf1N1B4_775 0.81 -1.0
880,624 - Pf1N1B4_775 0.81 -1.0
880,624 - Pf1N1B4_775 0.81 -1.6
880,752 - Pf1N1B4_775 0.87 -1.8
880,954 - -2.6
880,986 + -0.4
881,001 + -0.6
881,001 + -1.6
881,002 - -3.6
881,002 - -0.4
881,002 - -2.3
881,284 + Pf1N1B4_776 0.22 -2.6
881,382 - Pf1N1B4_776 0.30 -2.0
881,782 - Pf1N1B4_776 0.60 -1.8
881,782 - Pf1N1B4_776 0.60 -1.6
881,896 + Pf1N1B4_776 0.68 -0.6
881,897 - Pf1N1B4_776 0.68 -2.3
881,897 - Pf1N1B4_776 0.68 -2.8
881,897 - Pf1N1B4_776 0.68 -1.0
881,897 - Pf1N1B4_776 0.68 +0.0
881,897 - Pf1N1B4_776 0.68 -1.0
881,915 + Pf1N1B4_776 0.70 -1.6
882,298 + -1.6
882,299 - -1.9
882,355 + Pf1N1B4_777 0.12 -0.6
882,390 + Pf1N1B4_777 0.22 -1.0
882,391 - Pf1N1B4_777 0.22 -2.0
882,391 - Pf1N1B4_777 0.22 +0.0
882,391 - Pf1N1B4_777 0.22 -0.6
882,393 + Pf1N1B4_777 0.23 +0.3
882,393 + Pf1N1B4_777 0.23 +1.6
882,444 + Pf1N1B4_777 0.38 -0.7
882,444 + Pf1N1B4_777 0.38 +0.0
882,444 + Pf1N1B4_777 0.38 +0.7
882,444 + Pf1N1B4_777 0.38 -0.8
882,444 + Pf1N1B4_777 0.38 +0.3
882,444 + Pf1N1B4_777 0.38 -2.0
882,444 + Pf1N1B4_777 0.38 +0.0
882,444 + Pf1N1B4_777 0.38 +0.0
882,444 + Pf1N1B4_777 0.38 +0.2
882,444 + Pf1N1B4_777 0.38 -0.8
882,444 + Pf1N1B4_777 0.38 +1.0
882,445 - Pf1N1B4_777 0.38 +0.2
882,445 - Pf1N1B4_777 0.38 -0.6
882,445 - Pf1N1B4_777 0.38 +0.7
882,445 - Pf1N1B4_777 0.38 -1.0
882,445 - Pf1N1B4_777 0.38 +0.0
882,519 + Pf1N1B4_777 0.60 -1.0
882,519 + Pf1N1B4_777 0.60 -0.6
882,519 + Pf1N1B4_777 0.60 +0.5
882,519 + Pf1N1B4_777 0.60 -0.7
882,519 + Pf1N1B4_777 0.60 +0.0
882,529 - Pf1N1B4_777 0.63 +0.0
882,543 + Pf1N1B4_777 0.67 -1.3
882,543 + Pf1N1B4_777 0.67 -1.0
882,543 + Pf1N1B4_777 0.67 +0.0
882,543 + Pf1N1B4_777 0.67 +1.0
882,543 + Pf1N1B4_777 0.67 +0.6
882,543 + Pf1N1B4_777 0.67 +0.0
882,543 + Pf1N1B4_777 0.67 -0.4
882,543 + Pf1N1B4_777 0.67 -1.3
882,543 + Pf1N1B4_777 0.67 +0.0
882,544 - Pf1N1B4_777 0.68 +0.4
882,544 - Pf1N1B4_777 0.68 -1.0
882,544 - Pf1N1B4_777 0.68 -1.0
882,544 - +0.0
882,544 - Pf1N1B4_777 0.68 +0.3
882,544 - Pf1N1B4_777 0.68 +0.0
882,544 - Pf1N1B4_777 0.68 +0.7
882,544 - Pf1N1B4_777 0.68 +1.0
882,544 - Pf1N1B4_777 0.68 +0.4
882,544 - Pf1N1B4_777 0.68 -2.0
882,745 - +0.9
882,752 + -0.6
882,752 + -2.0
882,753 - -0.6
882,872 + Pf1N1B4_778 0.11 -0.4
882,872 + Pf1N1B4_778 0.11 +1.0
882,872 + Pf1N1B4_778 0.11 +1.0
882,872 + Pf1N1B4_778 0.11 +2.8
882,873 - +2.0
882,873 - Pf1N1B4_778 0.11 +2.3
882,873 - Pf1N1B4_778 0.11 -0.6
882,873 - Pf1N1B4_778 0.11 -0.1
882,873 - Pf1N1B4_778 0.11 -0.6
882,873 - Pf1N1B4_778 0.11 -1.0
882,873 - Pf1N1B4_778 0.11 +0.0
882,876 + Pf1N1B4_778 0.12 +0.6
882,932 + Pf1N1B4_778 0.18 +1.0
882,932 + Pf1N1B4_778 0.18 -0.6
882,932 + Pf1N1B4_778 0.18 -1.6
882,932 + Pf1N1B4_778 0.18 -0.4
882,932 + Pf1N1B4_778 0.18 +0.0
882,933 - Pf1N1B4_778 0.18 +0.3
883,210 + -1.0

Or see this region's nucleotide sequence