Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_775

Experiment: L-Asparagine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_773 and Pf1N1B4_774 overlap by 4 nucleotidesPf1N1B4_774 and Pf1N1B4_775 are separated by 223 nucleotidesPf1N1B4_775 and Pf1N1B4_776 overlap by 4 nucleotides Pf1N1B4_773 - ABC transporter for L-asparagine and L-glutamate, permease subunit 2 (from data), at 877,463 to 878,134 _773 Pf1N1B4_774 - ABC transporter for L-asparagine and L-glutamate, ATPase component (from data), at 878,131 to 878,865 _774 Pf1N1B4_775 - Signal transduction histidine kinase regulating C4-dicarboxylate transport system (EC 2.7.13.3), at 879,089 to 880,990 _775 Pf1N1B4_776 - Two component, Sigma-54 Specific, central transcriptional regulator of acidic amino acid uptake, at 880,987 to 882,315 _776 Position (kb) 879 880 881Strain fitness (log2 ratio) -3 -2 -1 0 1at 878.130 kb on + strandat 878.139 kb on + strandat 878.140 kb on - strandat 878.655 kb on - strand, within Pf1N1B4_774at 878.864 kb on - strandat 879.049 kb on - strandat 879.049 kb on - strandat 879.049 kb on - strandat 879.049 kb on - strandat 879.049 kb on - strandat 879.049 kb on - strandat 879.050 kb on + strandat 879.051 kb on - strandat 879.051 kb on - strandat 879.056 kb on + strandat 879.056 kb on + strandat 879.056 kb on + strandat 879.056 kb on + strandat 879.057 kb on - strandat 879.206 kb on + strandat 879.207 kb on - strandat 879.207 kb on - strandat 879.314 kb on + strand, within Pf1N1B4_775at 879.314 kb on + strand, within Pf1N1B4_775at 879.314 kb on + strand, within Pf1N1B4_775at 879.314 kb on + strand, within Pf1N1B4_775at 879.314 kb on + strand, within Pf1N1B4_775at 879.314 kb on + strand, within Pf1N1B4_775at 879.314 kb on + strand, within Pf1N1B4_775at 879.315 kb on - strand, within Pf1N1B4_775at 879.315 kb on - strand, within Pf1N1B4_775at 879.315 kb on - strand, within Pf1N1B4_775at 879.315 kb on - strand, within Pf1N1B4_775at 879.315 kb on - strand, within Pf1N1B4_775at 879.315 kb on - strand, within Pf1N1B4_775at 879.315 kb on - strand, within Pf1N1B4_775at 879.315 kb on - strand, within Pf1N1B4_775at 879.315 kb on - strand, within Pf1N1B4_775at 879.320 kb on + strand, within Pf1N1B4_775at 879.321 kb on - strand, within Pf1N1B4_775at 879.321 kb on - strand, within Pf1N1B4_775at 879.407 kb on + strand, within Pf1N1B4_775at 879.407 kb on + strand, within Pf1N1B4_775at 879.407 kb on + strand, within Pf1N1B4_775at 879.408 kb on - strand, within Pf1N1B4_775at 879.408 kb on - strand, within Pf1N1B4_775at 879.408 kb on - strand, within Pf1N1B4_775at 879.449 kb on + strand, within Pf1N1B4_775at 879.449 kb on + strand, within Pf1N1B4_775at 879.450 kb on - strand, within Pf1N1B4_775at 879.450 kb on - strandat 879.509 kb on + strand, within Pf1N1B4_775at 879.509 kb on + strand, within Pf1N1B4_775at 879.509 kb on + strand, within Pf1N1B4_775at 879.509 kb on + strand, within Pf1N1B4_775at 879.509 kb on + strand, within Pf1N1B4_775at 879.509 kb on + strand, within Pf1N1B4_775at 879.509 kb on + strand, within Pf1N1B4_775at 879.509 kb on + strand, within Pf1N1B4_775at 879.510 kb on - strand, within Pf1N1B4_775at 879.539 kb on + strand, within Pf1N1B4_775at 879.615 kb on - strand, within Pf1N1B4_775at 879.779 kb on - strand, within Pf1N1B4_775at 879.837 kb on - strand, within Pf1N1B4_775at 879.837 kb on - strand, within Pf1N1B4_775at 879.956 kb on + strand, within Pf1N1B4_775at 879.956 kb on + strand, within Pf1N1B4_775at 879.956 kb on + strand, within Pf1N1B4_775at 879.956 kb on + strand, within Pf1N1B4_775at 879.956 kb on + strand, within Pf1N1B4_775at 879.957 kb on - strand, within Pf1N1B4_775at 879.957 kb on - strand, within Pf1N1B4_775at 879.957 kb on - strand, within Pf1N1B4_775at 880.623 kb on + strand, within Pf1N1B4_775at 880.624 kb on - strand, within Pf1N1B4_775at 880.624 kb on - strand, within Pf1N1B4_775at 880.624 kb on - strand, within Pf1N1B4_775at 880.752 kb on - strand, within Pf1N1B4_775at 880.954 kb on - strandat 880.986 kb on + strandat 881.001 kb on + strandat 881.001 kb on + strandat 881.002 kb on - strandat 881.002 kb on - strandat 881.002 kb on - strandat 881.284 kb on + strand, within Pf1N1B4_776at 881.382 kb on - strand, within Pf1N1B4_776at 881.782 kb on - strand, within Pf1N1B4_776at 881.782 kb on - strand, within Pf1N1B4_776at 881.896 kb on + strand, within Pf1N1B4_776at 881.897 kb on - strand, within Pf1N1B4_776at 881.897 kb on - strand, within Pf1N1B4_776at 881.897 kb on - strand, within Pf1N1B4_776at 881.897 kb on - strand, within Pf1N1B4_776at 881.897 kb on - strand, within Pf1N1B4_776at 881.915 kb on + strand, within Pf1N1B4_776

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Asparagine (C)
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878,130 + -1.0
878,139 + -0.6
878,140 - -2.6
878,655 - Pf1N1B4_774 0.71 -0.5
878,864 - -1.6
879,049 - -2.0
879,049 - -1.2
879,049 - -1.0
879,049 - -1.6
879,049 - +0.0
879,049 - -1.6
879,050 + -2.0
879,051 - -1.8
879,051 - -2.0
879,056 + -1.6
879,056 + +0.0
879,056 + +0.9
879,056 + -1.0
879,057 - -1.9
879,206 + +1.0
879,207 - -2.0
879,207 - -2.6
879,314 + Pf1N1B4_775 0.12 -3.5
879,314 + Pf1N1B4_775 0.12 -1.0
879,314 + Pf1N1B4_775 0.12 +0.0
879,314 + Pf1N1B4_775 0.12 -0.6
879,314 + Pf1N1B4_775 0.12 -1.6
879,314 + Pf1N1B4_775 0.12 -2.8
879,314 + Pf1N1B4_775 0.12 -1.6
879,315 - Pf1N1B4_775 0.12 +0.0
879,315 - Pf1N1B4_775 0.12 +0.0
879,315 - Pf1N1B4_775 0.12 -2.8
879,315 - Pf1N1B4_775 0.12 -1.0
879,315 - Pf1N1B4_775 0.12 +0.0
879,315 - Pf1N1B4_775 0.12 +0.0
879,315 - Pf1N1B4_775 0.12 -0.2
879,315 - Pf1N1B4_775 0.12 +0.6
879,315 - Pf1N1B4_775 0.12 +1.0
879,320 + Pf1N1B4_775 0.12 -0.6
879,321 - Pf1N1B4_775 0.12 -2.3
879,321 - Pf1N1B4_775 0.12 -2.8
879,407 + Pf1N1B4_775 0.17 -2.6
879,407 + Pf1N1B4_775 0.17 +1.0
879,407 + Pf1N1B4_775 0.17 -1.0
879,408 - Pf1N1B4_775 0.17 -1.6
879,408 - Pf1N1B4_775 0.17 -2.5
879,408 - Pf1N1B4_775 0.17 -1.0
879,449 + Pf1N1B4_775 0.19 -0.7
879,449 + Pf1N1B4_775 0.19 -3.0
879,450 - Pf1N1B4_775 0.19 -1.0
879,450 - -1.0
879,509 + Pf1N1B4_775 0.22 -2.0
879,509 + Pf1N1B4_775 0.22 -0.6
879,509 + Pf1N1B4_775 0.22 -1.0
879,509 + Pf1N1B4_775 0.22 -1.0
879,509 + Pf1N1B4_775 0.22 -1.0
879,509 + Pf1N1B4_775 0.22 -1.6
879,509 + Pf1N1B4_775 0.22 +0.0
879,509 + Pf1N1B4_775 0.22 +0.0
879,510 - Pf1N1B4_775 0.22 -1.6
879,539 + Pf1N1B4_775 0.24 -2.3
879,615 - Pf1N1B4_775 0.28 -1.0
879,779 - Pf1N1B4_775 0.36 -1.6
879,837 - Pf1N1B4_775 0.39 -1.6
879,837 - Pf1N1B4_775 0.39 +0.0
879,956 + Pf1N1B4_775 0.46 -3.0
879,956 + Pf1N1B4_775 0.46 -0.3
879,956 + Pf1N1B4_775 0.46 +0.0
879,956 + Pf1N1B4_775 0.46 -2.3
879,956 + Pf1N1B4_775 0.46 -2.5
879,957 - Pf1N1B4_775 0.46 -2.6
879,957 - Pf1N1B4_775 0.46 -1.6
879,957 - Pf1N1B4_775 0.46 -2.0
880,623 + Pf1N1B4_775 0.81 +0.6
880,624 - Pf1N1B4_775 0.81 -1.0
880,624 - Pf1N1B4_775 0.81 -1.0
880,624 - Pf1N1B4_775 0.81 -1.6
880,752 - Pf1N1B4_775 0.87 -1.8
880,954 - -2.6
880,986 + -0.4
881,001 + -0.6
881,001 + -1.6
881,002 - -3.6
881,002 - -0.4
881,002 - -2.3
881,284 + Pf1N1B4_776 0.22 -2.6
881,382 - Pf1N1B4_776 0.30 -2.0
881,782 - Pf1N1B4_776 0.60 -1.8
881,782 - Pf1N1B4_776 0.60 -1.6
881,896 + Pf1N1B4_776 0.68 -0.6
881,897 - Pf1N1B4_776 0.68 -2.3
881,897 - Pf1N1B4_776 0.68 -2.8
881,897 - Pf1N1B4_776 0.68 -1.0
881,897 - Pf1N1B4_776 0.68 +0.0
881,897 - Pf1N1B4_776 0.68 -1.0
881,915 + Pf1N1B4_776 0.70 -1.6

Or see this region's nucleotide sequence