Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_773

Experiment: L-Asparagine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_771 and Pf1N1B4_772 are separated by 193 nucleotidesPf1N1B4_772 and Pf1N1B4_773 overlap by 1 nucleotidesPf1N1B4_773 and Pf1N1B4_774 overlap by 4 nucleotidesPf1N1B4_774 and Pf1N1B4_775 are separated by 223 nucleotides Pf1N1B4_771 - ABC transporter for L-asparagine and L-glutamate, periplasmic substrate-binding component (from data), at 875,609 to 876,523 _771 Pf1N1B4_772 - ABC transporter for L-asparagine and L-glutamate, permease component 1 (from data), at 876,717 to 877,463 _772 Pf1N1B4_773 - ABC transporter for L-asparagine and L-glutamate, permease subunit 2 (from data), at 877,463 to 878,134 _773 Pf1N1B4_774 - ABC transporter for L-asparagine and L-glutamate, ATPase component (from data), at 878,131 to 878,865 _774 Pf1N1B4_775 - Signal transduction histidine kinase regulating C4-dicarboxylate transport system (EC 2.7.13.3), at 879,089 to 880,990 _775 Position (kb) 877 878 879Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 876.687 kb on + strandat 876.687 kb on + strandat 876.687 kb on + strandat 876.687 kb on + strandat 876.688 kb on - strandat 876.688 kb on - strandat 876.723 kb on + strandat 876.724 kb on - strandat 876.724 kb on - strandat 876.724 kb on - strandat 876.777 kb on + strandat 876.777 kb on + strandat 876.777 kb on + strandat 876.778 kb on - strandat 876.789 kb on + strandat 876.789 kb on + strandat 876.790 kb on - strandat 876.919 kb on - strand, within Pf1N1B4_772at 876.919 kb on - strand, within Pf1N1B4_772at 876.919 kb on - strand, within Pf1N1B4_772at 876.919 kb on - strand, within Pf1N1B4_772at 876.970 kb on - strandat 877.014 kb on + strand, within Pf1N1B4_772at 877.014 kb on + strand, within Pf1N1B4_772at 877.014 kb on + strand, within Pf1N1B4_772at 877.014 kb on + strand, within Pf1N1B4_772at 877.015 kb on - strandat 877.015 kb on - strandat 877.015 kb on - strand, within Pf1N1B4_772at 877.182 kb on + strand, within Pf1N1B4_772at 877.182 kb on + strand, within Pf1N1B4_772at 877.183 kb on - strand, within Pf1N1B4_772at 877.232 kb on + strand, within Pf1N1B4_772at 877.252 kb on + strand, within Pf1N1B4_772at 877.253 kb on - strand, within Pf1N1B4_772at 877.253 kb on - strand, within Pf1N1B4_772at 877.371 kb on + strand, within Pf1N1B4_772at 877.371 kb on + strand, within Pf1N1B4_772at 877.371 kb on + strand, within Pf1N1B4_772at 877.372 kb on - strand, within Pf1N1B4_772at 877.372 kb on - strand, within Pf1N1B4_772at 877.372 kb on - strand, within Pf1N1B4_772at 877.372 kb on - strand, within Pf1N1B4_772at 877.372 kb on - strand, within Pf1N1B4_772at 877.635 kb on - strand, within Pf1N1B4_773at 877.639 kb on + strand, within Pf1N1B4_773at 877.644 kb on - strand, within Pf1N1B4_773at 877.644 kb on - strand, within Pf1N1B4_773at 877.644 kb on - strand, within Pf1N1B4_773at 877.644 kb on - strand, within Pf1N1B4_773at 877.685 kb on + strand, within Pf1N1B4_773at 877.686 kb on - strand, within Pf1N1B4_773at 877.704 kb on + strand, within Pf1N1B4_773at 877.704 kb on + strand, within Pf1N1B4_773at 877.704 kb on + strand, within Pf1N1B4_773at 877.705 kb on - strand, within Pf1N1B4_773at 877.705 kb on - strand, within Pf1N1B4_773at 877.705 kb on - strand, within Pf1N1B4_773at 877.707 kb on + strandat 877.707 kb on + strand, within Pf1N1B4_773at 877.787 kb on + strand, within Pf1N1B4_773at 877.869 kb on - strand, within Pf1N1B4_773at 878.069 kb on + strandat 878.069 kb on + strandat 878.069 kb on + strandat 878.130 kb on + strandat 878.139 kb on + strandat 878.140 kb on - strandat 878.655 kb on - strand, within Pf1N1B4_774at 878.864 kb on - strandat 879.049 kb on - strandat 879.049 kb on - strandat 879.049 kb on - strandat 879.049 kb on - strandat 879.049 kb on - strandat 879.049 kb on - strandat 879.050 kb on + strandat 879.051 kb on - strandat 879.051 kb on - strandat 879.056 kb on + strandat 879.056 kb on + strandat 879.056 kb on + strandat 879.056 kb on + strandat 879.057 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Asparagine (C)
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876,687 + +0.3
876,687 + +0.0
876,687 + -1.8
876,687 + -0.6
876,688 - -0.4
876,688 - +0.0
876,723 + -2.3
876,724 - -0.4
876,724 - -1.3
876,724 - -0.6
876,777 + -1.6
876,777 + -3.0
876,777 + -1.0
876,778 - +0.0
876,789 + -2.3
876,789 + -0.4
876,790 - -2.0
876,919 - Pf1N1B4_772 0.27 -1.6
876,919 - Pf1N1B4_772 0.27 -2.6
876,919 - Pf1N1B4_772 0.27 -3.2
876,919 - Pf1N1B4_772 0.27 -1.0
876,970 - -1.0
877,014 + Pf1N1B4_772 0.40 -1.0
877,014 + Pf1N1B4_772 0.40 -1.6
877,014 + Pf1N1B4_772 0.40 -1.6
877,014 + Pf1N1B4_772 0.40 -2.0
877,015 - +0.0
877,015 - +0.0
877,015 - Pf1N1B4_772 0.40 -2.0
877,182 + Pf1N1B4_772 0.62 -2.3
877,182 + Pf1N1B4_772 0.62 -0.6
877,183 - Pf1N1B4_772 0.62 -2.3
877,232 + Pf1N1B4_772 0.69 -1.0
877,252 + Pf1N1B4_772 0.72 -0.6
877,253 - Pf1N1B4_772 0.72 -1.0
877,253 - Pf1N1B4_772 0.72 +2.0
877,371 + Pf1N1B4_772 0.88 -1.6
877,371 + Pf1N1B4_772 0.88 -2.0
877,371 + Pf1N1B4_772 0.88 -1.6
877,372 - Pf1N1B4_772 0.88 -1.0
877,372 - Pf1N1B4_772 0.88 -1.0
877,372 - Pf1N1B4_772 0.88 -1.6
877,372 - Pf1N1B4_772 0.88 -0.4
877,372 - Pf1N1B4_772 0.88 -1.6
877,635 - Pf1N1B4_773 0.26 -2.0
877,639 + Pf1N1B4_773 0.26 -2.0
877,644 - Pf1N1B4_773 0.27 -0.7
877,644 - Pf1N1B4_773 0.27 -1.6
877,644 - Pf1N1B4_773 0.27 -1.0
877,644 - Pf1N1B4_773 0.27 -1.6
877,685 + Pf1N1B4_773 0.33 -1.3
877,686 - Pf1N1B4_773 0.33 -2.0
877,704 + Pf1N1B4_773 0.36 +1.0
877,704 + Pf1N1B4_773 0.36 -2.8
877,704 + Pf1N1B4_773 0.36 -0.8
877,705 - Pf1N1B4_773 0.36 +0.0
877,705 - Pf1N1B4_773 0.36 -1.6
877,705 - Pf1N1B4_773 0.36 -0.6
877,707 + +0.0
877,707 + Pf1N1B4_773 0.36 -1.0
877,787 + Pf1N1B4_773 0.48 +1.0
877,869 - Pf1N1B4_773 0.60 -2.7
878,069 + +0.0
878,069 + +0.0
878,069 + +0.0
878,130 + -1.0
878,139 + -0.6
878,140 - -2.6
878,655 - Pf1N1B4_774 0.71 -0.5
878,864 - -1.6
879,049 - -2.0
879,049 - -1.2
879,049 - -1.0
879,049 - -1.6
879,049 - +0.0
879,049 - -1.6
879,050 + -2.0
879,051 - -1.8
879,051 - -2.0
879,056 + -1.6
879,056 + +0.0
879,056 + +0.9
879,056 + -1.0
879,057 - -1.9

Or see this region's nucleotide sequence