Experiment: L-Asparagine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1350 and Pf1N1B4_1351 are separated by 5 nucleotides Pf1N1B4_1351 and Pf1N1B4_1352 overlap by 1 nucleotides Pf1N1B4_1352 and Pf1N1B4_1353 are separated by 126 nucleotides Pf1N1B4_1353 and Pf1N1B4_1354 are separated by 67 nucleotides
Pf1N1B4_1350 - GCN5-related N-acetyltransferase, at 1,546,404 to 1,546,937
_1350
Pf1N1B4_1351 - Tabtoxin resistance protein, at 1,546,943 to 1,547,476
_1351
Pf1N1B4_1352 - Urease beta subunit (EC 3.5.1.5), at 1,547,476 to 1,547,784
_1352
Pf1N1B4_1353 - Urease alpha subunit (EC 3.5.1.5), at 1,547,911 to 1,549,611
_1353
Pf1N1B4_1354 - hypothetical protein, at 1,549,679 to 1,552,135
_1354
Position (kb)
1547
1548
1549
1550 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1546.939 kb on - strand at 1547.087 kb on + strand, within Pf1N1B4_1351 at 1547.088 kb on - strand, within Pf1N1B4_1351 at 1547.324 kb on + strand, within Pf1N1B4_1351 at 1547.324 kb on + strand, within Pf1N1B4_1351 at 1547.324 kb on + strand, within Pf1N1B4_1351 at 1547.325 kb on - strand, within Pf1N1B4_1351 at 1547.364 kb on - strand, within Pf1N1B4_1351 at 1547.364 kb on - strand, within Pf1N1B4_1351 at 1547.364 kb on - strand, within Pf1N1B4_1351 at 1547.364 kb on - strand at 1547.448 kb on - strand at 1547.450 kb on + strand at 1547.450 kb on + strand at 1547.450 kb on + strand at 1547.451 kb on - strand at 1547.451 kb on - strand at 1547.451 kb on - strand at 1547.451 kb on - strand at 1547.593 kb on + strand, within Pf1N1B4_1352 at 1547.782 kb on + strand at 1547.933 kb on - strand at 1548.023 kb on - strand at 1548.309 kb on + strand, within Pf1N1B4_1353 at 1548.718 kb on + strand, within Pf1N1B4_1353 at 1548.719 kb on - strand, within Pf1N1B4_1353 at 1548.719 kb on - strand, within Pf1N1B4_1353 at 1548.785 kb on + strand, within Pf1N1B4_1353 at 1548.786 kb on - strand, within Pf1N1B4_1353 at 1548.786 kb on - strand, within Pf1N1B4_1353 at 1548.786 kb on - strand, within Pf1N1B4_1353 at 1549.114 kb on + strand, within Pf1N1B4_1353 at 1549.115 kb on - strand, within Pf1N1B4_1353 at 1549.115 kb on - strand, within Pf1N1B4_1353 at 1549.126 kb on + strand, within Pf1N1B4_1353 at 1549.126 kb on + strand, within Pf1N1B4_1353 at 1549.126 kb on + strand, within Pf1N1B4_1353 at 1549.126 kb on + strand, within Pf1N1B4_1353 at 1549.233 kb on - strand, within Pf1N1B4_1353 at 1549.495 kb on + strand at 1549.496 kb on - strand at 1549.496 kb on - strand at 1549.528 kb on + strand at 1549.528 kb on + strand at 1549.528 kb on + strand at 1549.528 kb on + strand at 1549.528 kb on + strand at 1549.529 kb on - strand at 1549.529 kb on - strand at 1549.556 kb on + strand at 1549.597 kb on + strand at 1549.597 kb on + strand at 1549.597 kb on + strand at 1549.598 kb on - strand at 1549.620 kb on + strand at 1549.620 kb on + strand at 1549.620 kb on + strand at 1549.621 kb on - strand at 1549.736 kb on - strand at 1549.739 kb on + strand at 1549.740 kb on - strand at 1549.740 kb on - strand at 1549.740 kb on - strand at 1549.740 kb on - strand at 1549.740 kb on - strand at 1549.740 kb on - strand at 1549.740 kb on - strand at 1549.740 kb on - strand at 1549.740 kb on - strand at 1549.791 kb on + strand at 1549.919 kb on + strand at 1549.919 kb on + strand at 1549.935 kb on - strand, within Pf1N1B4_1354 at 1549.935 kb on - strand, within Pf1N1B4_1354 at 1550.179 kb on - strand, within Pf1N1B4_1354 at 1550.179 kb on - strand, within Pf1N1B4_1354 at 1550.374 kb on - strand, within Pf1N1B4_1354 at 1550.374 kb on - strand, within Pf1N1B4_1354 at 1550.374 kb on - strand, within Pf1N1B4_1354 at 1550.374 kb on - strand, within Pf1N1B4_1354 at 1550.374 kb on - strand, within Pf1N1B4_1354
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (C) remove 1,546,939 - +0.2 1,547,087 + Pf1N1B4_1351 0.27 +0.6 1,547,088 - Pf1N1B4_1351 0.27 +0.8 1,547,324 + Pf1N1B4_1351 0.71 +0.9 1,547,324 + Pf1N1B4_1351 0.71 +0.9 1,547,324 + Pf1N1B4_1351 0.71 +0.8 1,547,325 - Pf1N1B4_1351 0.72 -0.7 1,547,364 - Pf1N1B4_1351 0.79 -0.8 1,547,364 - Pf1N1B4_1351 0.79 +1.2 1,547,364 - Pf1N1B4_1351 0.79 -0.3 1,547,364 - +1.2 1,547,448 - +0.8 1,547,450 + -0.8 1,547,450 + -0.8 1,547,450 + -0.8 1,547,451 - -0.4 1,547,451 - +0.2 1,547,451 - -0.4 1,547,451 - -1.8 1,547,593 + Pf1N1B4_1352 0.38 +3.0 1,547,782 + +1.0 1,547,933 - +0.4 1,548,023 - +0.8 1,548,309 + Pf1N1B4_1353 0.23 -0.8 1,548,718 + Pf1N1B4_1353 0.47 -0.8 1,548,719 - Pf1N1B4_1353 0.48 -0.9 1,548,719 - Pf1N1B4_1353 0.48 -0.4 1,548,785 + Pf1N1B4_1353 0.51 +0.8 1,548,786 - Pf1N1B4_1353 0.51 -0.5 1,548,786 - Pf1N1B4_1353 0.51 -0.1 1,548,786 - Pf1N1B4_1353 0.51 +0.5 1,549,114 + Pf1N1B4_1353 0.71 +0.2 1,549,115 - Pf1N1B4_1353 0.71 +1.2 1,549,115 - Pf1N1B4_1353 0.71 -0.5 1,549,126 + Pf1N1B4_1353 0.71 +0.2 1,549,126 + Pf1N1B4_1353 0.71 -0.8 1,549,126 + Pf1N1B4_1353 0.71 -1.4 1,549,126 + Pf1N1B4_1353 0.71 +0.6 1,549,233 - Pf1N1B4_1353 0.78 -1.8 1,549,495 + -1.4 1,549,496 - -1.0 1,549,496 - -0.2 1,549,528 + -0.2 1,549,528 + -0.8 1,549,528 + +0.2 1,549,528 + +0.5 1,549,528 + -0.2 1,549,529 - +0.2 1,549,529 - -1.0 1,549,556 + -1.2 1,549,597 + +0.2 1,549,597 + +0.5 1,549,597 + +0.9 1,549,598 - -0.8 1,549,620 + -0.2 1,549,620 + -0.2 1,549,620 + +0.2 1,549,621 - +1.2 1,549,736 - -0.2 1,549,739 + +0.8 1,549,740 - +1.1 1,549,740 - -1.4 1,549,740 - -0.0 1,549,740 - -0.4 1,549,740 - +0.2 1,549,740 - -0.0 1,549,740 - -0.8 1,549,740 - +0.5 1,549,740 - -1.0 1,549,791 + -0.8 1,549,919 + +0.3 1,549,919 + -1.4 1,549,935 - Pf1N1B4_1354 0.10 -0.8 1,549,935 - Pf1N1B4_1354 0.10 -0.1 1,550,179 - Pf1N1B4_1354 0.20 +0.7 1,550,179 - Pf1N1B4_1354 0.20 +1.4 1,550,374 - Pf1N1B4_1354 0.28 +1.8 1,550,374 - Pf1N1B4_1354 0.28 +0.7 1,550,374 - Pf1N1B4_1354 0.28 -0.0 1,550,374 - Pf1N1B4_1354 0.28 -0.1 1,550,374 - Pf1N1B4_1354 0.28 -0.2
Or see this region's nucleotide sequence