Experiment: A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3523 and Shewana3_3524 are separated by 144 nucleotides Shewana3_3524 and Shewana3_3525 are separated by 34 nucleotides Shewana3_3525 and Shewana3_3526 are separated by 82 nucleotides
Shewana3_3523: Shewana3_3523 - ferredoxin-dependent glutamate synthase (RefSeq), at 4,223,364 to 4,224,854
_3523
Shewana3_3524: Shewana3_3524 - hypothetical protein (RefSeq), at 4,224,999 to 4,225,205
_3524
Shewana3_3525: Shewana3_3525 - hypothetical protein (RefSeq), at 4,225,240 to 4,225,497
_3525
Shewana3_3526: Shewana3_3526 - ABC transporter related (RefSeq), at 4,225,580 to 4,227,376
_3526
Position (kb)
4225
4226 Strain fitness (log2 ratio)
-2
-1
0
1 at 4224.265 kb on - strand, within Shewana3_3523 at 4224.672 kb on - strand, within Shewana3_3523 at 4224.732 kb on - strand at 4224.732 kb on - strand at 4224.732 kb on - strand at 4224.743 kb on + strand at 4224.751 kb on - strand at 4224.760 kb on + strand at 4224.778 kb on - strand at 4224.944 kb on + strand at 4224.971 kb on - strand at 4225.334 kb on - strand, within Shewana3_3525 at 4225.347 kb on + strand, within Shewana3_3525 at 4225.360 kb on + strand, within Shewana3_3525 at 4225.360 kb on + strand, within Shewana3_3525 at 4225.368 kb on - strand, within Shewana3_3525 at 4225.370 kb on + strand, within Shewana3_3525 at 4225.541 kb on - strand at 4225.917 kb on - strand, within Shewana3_3526 at 4226.161 kb on - strand, within Shewana3_3526 at 4226.313 kb on - strand, within Shewana3_3526 at 4226.362 kb on - strand, within Shewana3_3526 at 4226.403 kb on + strand, within Shewana3_3526 at 4226.408 kb on + strand, within Shewana3_3526 at 4226.486 kb on + strand, within Shewana3_3526 at 4226.491 kb on + strand, within Shewana3_3526
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.02 mg/ml remove 4,224,265 - Shewana3_3523 0.60 -0.8 4,224,672 - Shewana3_3523 0.88 +0.5 4,224,732 - -0.0 4,224,732 - +0.7 4,224,732 - -0.5 4,224,743 + -0.6 4,224,751 - +0.0 4,224,760 + -0.6 4,224,778 - +0.4 4,224,944 + -0.6 4,224,971 - -1.1 4,225,334 - Shewana3_3525 0.36 -0.6 4,225,347 + Shewana3_3525 0.41 -0.8 4,225,360 + Shewana3_3525 0.47 -0.1 4,225,360 + Shewana3_3525 0.47 +0.9 4,225,368 - Shewana3_3525 0.50 -1.3 4,225,370 + Shewana3_3525 0.50 -1.1 4,225,541 - -1.8 4,225,917 - Shewana3_3526 0.19 -0.6 4,226,161 - Shewana3_3526 0.32 -2.1 4,226,313 - Shewana3_3526 0.41 +0.4 4,226,362 - Shewana3_3526 0.44 +0.0 4,226,403 + Shewana3_3526 0.46 -1.1 4,226,408 + Shewana3_3526 0.46 -1.1 4,226,486 + Shewana3_3526 0.50 -0.1 4,226,491 + Shewana3_3526 0.51 -0.3
Or see this region's nucleotide sequence