Experiment: A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3462 and Shewana3_3463 are separated by 117 nucleotides Shewana3_3463 and Shewana3_3464 are separated by 126 nucleotides Shewana3_3464 and Shewana3_3465 are separated by 77 nucleotides
Shewana3_3462: Shewana3_3462 - alpha/beta hydrolase domain-containing protein (RefSeq), at 4,151,848 to 4,152,837
_3462
Shewana3_3463: Shewana3_3463 - hypothetical protein (RefSeq), at 4,152,955 to 4,153,410
_3463
Shewana3_3464: Shewana3_3464 - amidase (RefSeq), at 4,153,537 to 4,155,243
_3464
Shewana3_3465: Shewana3_3465 - agmatine deiminase (RefSeq), at 4,155,321 to 4,156,433
_3465
Position (kb) 4153
4154
4155
4156 Strain fitness (log2 ratio) -2
-1
0
1 at 4152.698 kb on + strand, within Shewana3_3462 at 4152.737 kb on + strand, within Shewana3_3462 at 4152.745 kb on - strand at 4152.844 kb on - strand at 4153.033 kb on - strand, within Shewana3_3463 at 4153.071 kb on + strand, within Shewana3_3463 at 4153.079 kb on - strand, within Shewana3_3463 at 4153.100 kb on + strand, within Shewana3_3463 at 4153.110 kb on + strand, within Shewana3_3463 at 4153.118 kb on - strand, within Shewana3_3463 at 4153.128 kb on + strand, within Shewana3_3463 at 4153.156 kb on - strand, within Shewana3_3463 at 4153.242 kb on + strand, within Shewana3_3463 at 4153.313 kb on + strand, within Shewana3_3463 at 4153.332 kb on + strand, within Shewana3_3463 at 4153.340 kb on - strand, within Shewana3_3463 at 4153.360 kb on + strand, within Shewana3_3463 at 4153.362 kb on + strand, within Shewana3_3463 at 4153.400 kb on + strand at 4153.641 kb on + strand at 4153.648 kb on + strand at 4153.648 kb on + strand at 4153.693 kb on - strand at 4153.759 kb on + strand, within Shewana3_3464 at 4153.903 kb on - strand, within Shewana3_3464 at 4154.012 kb on + strand, within Shewana3_3464 at 4154.020 kb on - strand, within Shewana3_3464 at 4154.403 kb on + strand, within Shewana3_3464 at 4154.411 kb on - strand, within Shewana3_3464 at 4154.685 kb on - strand, within Shewana3_3464 at 4154.998 kb on + strand, within Shewana3_3464 at 4155.029 kb on - strand, within Shewana3_3464 at 4155.029 kb on - strand, within Shewana3_3464 at 4155.091 kb on - strand at 4155.352 kb on - strand at 4155.468 kb on - strand, within Shewana3_3465 at 4155.543 kb on + strand, within Shewana3_3465 at 4155.563 kb on - strand, within Shewana3_3465 at 4155.586 kb on + strand, within Shewana3_3465 at 4155.848 kb on - strand, within Shewana3_3465 at 4155.877 kb on - strand, within Shewana3_3465 at 4156.092 kb on + strand, within Shewana3_3465 at 4156.192 kb on - strand, within Shewana3_3465 at 4156.233 kb on + strand, within Shewana3_3465
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.02 mg/ml remove 4,152,698 + Shewana3_3462 0.86 +0.9 4,152,737 + Shewana3_3462 0.90 -0.4 4,152,745 - -0.6 4,152,844 - -0.0 4,153,033 - Shewana3_3463 0.17 -0.5 4,153,071 + Shewana3_3463 0.25 -1.0 4,153,079 - Shewana3_3463 0.27 +0.3 4,153,100 + Shewana3_3463 0.32 -0.4 4,153,110 + Shewana3_3463 0.34 +0.6 4,153,118 - Shewana3_3463 0.36 -0.2 4,153,128 + Shewana3_3463 0.38 +0.2 4,153,156 - Shewana3_3463 0.44 +0.3 4,153,242 + Shewana3_3463 0.63 -0.1 4,153,313 + Shewana3_3463 0.79 -1.0 4,153,332 + Shewana3_3463 0.83 -0.2 4,153,340 - Shewana3_3463 0.84 +0.3 4,153,360 + Shewana3_3463 0.89 +0.0 4,153,362 + Shewana3_3463 0.89 +0.0 4,153,400 + -0.4 4,153,641 + -1.0 4,153,648 + -1.4 4,153,648 + -0.4 4,153,693 - -2.5 4,153,759 + Shewana3_3464 0.13 -1.0 4,153,903 - Shewana3_3464 0.21 -1.5 4,154,012 + Shewana3_3464 0.28 -0.4 4,154,020 - Shewana3_3464 0.28 -1.7 4,154,403 + Shewana3_3464 0.51 +0.9 4,154,411 - Shewana3_3464 0.51 -2.0 4,154,685 - Shewana3_3464 0.67 -2.1 4,154,998 + Shewana3_3464 0.86 -0.7 4,155,029 - Shewana3_3464 0.87 -1.4 4,155,029 - Shewana3_3464 0.87 -1.5 4,155,091 - -1.8 4,155,352 - -0.6 4,155,468 - Shewana3_3465 0.13 -0.4 4,155,543 + Shewana3_3465 0.20 +0.5 4,155,563 - Shewana3_3465 0.22 -0.3 4,155,586 + Shewana3_3465 0.24 +0.9 4,155,848 - Shewana3_3465 0.47 -0.5 4,155,877 - Shewana3_3465 0.50 -0.6 4,156,092 + Shewana3_3465 0.69 -0.7 4,156,192 - Shewana3_3465 0.78 -1.4 4,156,233 + Shewana3_3465 0.82 +0.7
Or see this region's nucleotide sequence