Experiment: A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2587 and Shewana3_2588 are separated by 149 nucleotides Shewana3_2588 and Shewana3_2589 are separated by 151 nucleotides Shewana3_2589 and Shewana3_2590 are separated by 77 nucleotides
Shewana3_2587: Shewana3_2587 - NnrS family protein (RefSeq), at 3,092,547 to 3,093,719
_2587
Shewana3_2588: Shewana3_2588 - phosphate-starvation-inducible E (RefSeq), at 3,093,869 to 3,094,273
_2588
Shewana3_2589: Shewana3_2589 - type IV pilus assembly PilZ (RefSeq), at 3,094,425 to 3,094,727
_2589
Shewana3_2590: Shewana3_2590 - hypothetical protein (RefSeq), at 3,094,805 to 3,095,473
_2590
Position (kb)
3093
3094
3095 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3092.891 kb on + strand, within Shewana3_2587 at 3093.127 kb on + strand, within Shewana3_2587 at 3093.227 kb on - strand, within Shewana3_2587 at 3093.615 kb on + strand at 3093.676 kb on + strand at 3094.124 kb on - strand, within Shewana3_2588 at 3094.300 kb on + strand at 3094.323 kb on - strand at 3094.497 kb on + strand, within Shewana3_2589 at 3094.505 kb on - strand, within Shewana3_2589 at 3094.530 kb on - strand, within Shewana3_2589 at 3094.686 kb on - strand, within Shewana3_2589 at 3094.686 kb on - strand, within Shewana3_2589 at 3094.760 kb on + strand at 3094.760 kb on + strand at 3095.074 kb on + strand, within Shewana3_2590 at 3095.077 kb on - strand, within Shewana3_2590 at 3095.127 kb on + strand, within Shewana3_2590 at 3095.203 kb on + strand, within Shewana3_2590 at 3095.220 kb on + strand, within Shewana3_2590 at 3095.220 kb on + strand, within Shewana3_2590 at 3095.228 kb on - strand, within Shewana3_2590
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.02 mg/ml remove 3,092,891 + Shewana3_2587 0.29 +0.6 3,093,127 + Shewana3_2587 0.49 -1.0 3,093,227 - Shewana3_2587 0.58 +2.2 3,093,615 + -0.1 3,093,676 + +0.4 3,094,124 - Shewana3_2588 0.63 +1.6 3,094,300 + +0.6 3,094,323 - +0.6 3,094,497 + Shewana3_2589 0.24 +0.6 3,094,505 - Shewana3_2589 0.26 +1.2 3,094,530 - Shewana3_2589 0.35 +0.6 3,094,686 - Shewana3_2589 0.86 +0.6 3,094,686 - Shewana3_2589 0.86 -3.2 3,094,760 + -0.4 3,094,760 + +0.6 3,095,074 + Shewana3_2590 0.40 +0.6 3,095,077 - Shewana3_2590 0.41 -1.4 3,095,127 + Shewana3_2590 0.48 -0.4 3,095,203 + Shewana3_2590 0.59 +0.6 3,095,220 + Shewana3_2590 0.62 +0.3 3,095,220 + Shewana3_2590 0.62 -0.4 3,095,228 - Shewana3_2590 0.63 +0.6
Or see this region's nucleotide sequence