Experiment: A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2387 and Shewana3_2388 are separated by 21 nucleotides Shewana3_2388 and Shewana3_2389 are separated by 130 nucleotides Shewana3_2389 and Shewana3_2390 overlap by 8 nucleotides Shewana3_2390 and Shewana3_2391 are separated by 142 nucleotides
Shewana3_2387: Shewana3_2387 - alpha/beta hydrolase fold (RefSeq), at 2,866,785 to 2,867,651
_2387
Shewana3_2388: Shewana3_2388 - hypothetical protein (RefSeq), at 2,867,673 to 2,868,485
_2388
Shewana3_2389: Shewana3_2389 - hypothetical protein (RefSeq), at 2,868,616 to 2,869,323
_2389
Shewana3_2390: Shewana3_2390 - hypothetical protein (RefSeq), at 2,869,316 to 2,869,624
_2390
Shewana3_2391: Shewana3_2391 - peptidase M22, glycoprotease (RefSeq), at 2,869,767 to 2,870,477
_2391
Position (kb)
2868
2869
2870 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2867.617 kb on - strand at 2867.751 kb on + strand at 2867.833 kb on - strand, within Shewana3_2388 at 2868.222 kb on + strand, within Shewana3_2388 at 2868.400 kb on + strand, within Shewana3_2388 at 2868.426 kb on + strand at 2868.434 kb on - strand at 2868.434 kb on - strand at 2868.434 kb on - strand at 2868.804 kb on + strand, within Shewana3_2389 at 2868.872 kb on + strand, within Shewana3_2389 at 2868.919 kb on + strand, within Shewana3_2389 at 2868.927 kb on - strand, within Shewana3_2389 at 2869.063 kb on + strand, within Shewana3_2389 at 2869.149 kb on + strand, within Shewana3_2389 at 2869.400 kb on + strand, within Shewana3_2390 at 2869.472 kb on + strand, within Shewana3_2390 at 2869.653 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.02 mg/ml remove 2,867,617 - -0.5 2,867,751 + -2.3 2,867,833 - Shewana3_2388 0.20 +0.8 2,868,222 + Shewana3_2388 0.68 -0.5 2,868,400 + Shewana3_2388 0.89 +0.5 2,868,426 + +0.5 2,868,434 - +2.1 2,868,434 - -1.8 2,868,434 - +1.5 2,868,804 + Shewana3_2389 0.27 +0.5 2,868,872 + Shewana3_2389 0.36 -0.5 2,868,919 + Shewana3_2389 0.43 +0.5 2,868,927 - Shewana3_2389 0.44 +0.5 2,869,063 + Shewana3_2389 0.63 +0.1 2,869,149 + Shewana3_2389 0.75 -1.6 2,869,400 + Shewana3_2390 0.27 +0.6 2,869,472 + Shewana3_2390 0.50 +1.0 2,869,653 - -1.5
Or see this region's nucleotide sequence