Experiment: A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2257 and Shewana3_2258 are separated by 72 nucleotides Shewana3_2258 and Shewana3_2259 are separated by 142 nucleotides Shewana3_2259 and Shewana3_2260 are separated by 87 nucleotides
Shewana3_2257: Shewana3_2257 - phosphoenolpyruvate--protein phosphotransferase (RefSeq), at 2,718,641 to 2,720,344
_2257
Shewana3_2258: Shewana3_2258 - glucose-specific PTS system component (RefSeq), at 2,720,417 to 2,720,926
_2258
Shewana3_2259: Shewana3_2259 - hypothetical protein (RefSeq), at 2,721,069 to 2,721,431
_2259
Shewana3_2260: Shewana3_2260 - hypothetical protein (RefSeq), at 2,721,519 to 2,722,034
_2260
Position (kb)
2721
2722 Strain fitness (log2 ratio)
-1
0
1 at 2720.107 kb on + strand, within Shewana3_2257 at 2720.307 kb on - strand at 2720.323 kb on + strand at 2720.323 kb on + strand at 2720.365 kb on + strand at 2720.769 kb on + strand, within Shewana3_2258 at 2721.000 kb on - strand at 2721.017 kb on - strand at 2721.147 kb on - strand, within Shewana3_2259 at 2721.476 kb on + strand at 2721.766 kb on - strand, within Shewana3_2260
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.02 mg/ml remove 2,720,107 + Shewana3_2257 0.86 -0.4 2,720,307 - +0.6 2,720,323 + +0.6 2,720,323 + -1.7 2,720,365 + +0.6 2,720,769 + Shewana3_2258 0.69 +0.6 2,721,000 - -0.4 2,721,017 - +0.6 2,721,147 - Shewana3_2259 0.21 -1.0 2,721,476 + +0.6 2,721,766 - Shewana3_2260 0.48 +0.6
Or see this region's nucleotide sequence