Experiment: A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1604 and Shewana3_1605 are separated by 187 nucleotides Shewana3_1605 and Shewana3_1606 are separated by 107 nucleotides Shewana3_1606 and Shewana3_1607 are separated by 74 nucleotides
Shewana3_1604: Shewana3_1604 - hypothetical protein (RefSeq), at 1,882,924 to 1,884,747
_1604
Shewana3_1605: Shewana3_1605 - ribosome modulation factor (RefSeq), at 1,884,935 to 1,885,111
_1605
Shewana3_1606: Shewana3_1606 - 3-hydroxydecanoyl-(acyl carrier protein) dehydratase (RefSeq), at 1,885,219 to 1,885,734
_1606
Shewana3_1607: Shewana3_1607 - ATP-dependent protease (RefSeq), at 1,885,809 to 1,887,557
_1607
Position (kb)
1884
1885
1886 Strain fitness (log2 ratio)
-1
0
1
2 at 1884.392 kb on + strand, within Shewana3_1604 at 1884.587 kb on + strand at 1884.643 kb on + strand at 1884.918 kb on + strand at 1885.013 kb on - strand, within Shewana3_1605 at 1885.051 kb on + strand, within Shewana3_1605 at 1885.150 kb on + strand at 1885.215 kb on - strand at 1885.320 kb on - strand, within Shewana3_1606 at 1885.386 kb on - strand, within Shewana3_1606 at 1885.417 kb on - strand, within Shewana3_1606 at 1885.417 kb on - strand, within Shewana3_1606 at 1885.434 kb on - strand, within Shewana3_1606
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.02 mg/ml remove 1,884,392 + Shewana3_1604 0.80 -1.4 1,884,587 + -1.2 1,884,643 + -1.5 1,884,918 + +1.9 1,885,013 - Shewana3_1605 0.44 -0.5 1,885,051 + Shewana3_1605 0.66 -0.9 1,885,150 + +1.5 1,885,215 - -0.2 1,885,320 - Shewana3_1606 0.20 -1.2 1,885,386 - Shewana3_1606 0.32 +0.3 1,885,417 - Shewana3_1606 0.38 +0.3 1,885,417 - Shewana3_1606 0.38 -0.1 1,885,434 - Shewana3_1606 0.42 -0.9
Or see this region's nucleotide sequence