Experiment: A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0401 and Shewana3_0402 are separated by 206 nucleotides Shewana3_0402 and Shewana3_0403 are separated by 61 nucleotides
Shewana3_0401: Shewana3_0401 - fumarate reductase cytochrome b-556 subunit (RefSeq), at 460,293 to 461,018
_0401
Shewana3_0402: Shewana3_0402 - fumarate reductase respiratory complex, transmembrane subunit (RefSeq), at 461,225 to 461,896
_0402
Shewana3_0403: Shewana3_0403 - fumarate reductase flavoprotein subunit (RefSeq), at 461,958 to 463,964
_0403
Position (kb) 461
462 Strain fitness (log2 ratio) -2
-1
0
1
2 at 460.344 kb on - strand at 460.368 kb on + strand, within Shewana3_0401 at 460.387 kb on + strand, within Shewana3_0401 at 460.395 kb on - strand, within Shewana3_0401 at 460.395 kb on - strand, within Shewana3_0401 at 460.402 kb on + strand, within Shewana3_0401 at 460.501 kb on + strand, within Shewana3_0401 at 460.514 kb on - strand, within Shewana3_0401 at 460.514 kb on - strand, within Shewana3_0401 at 460.650 kb on - strand, within Shewana3_0401 at 460.729 kb on - strand, within Shewana3_0401 at 460.799 kb on + strand, within Shewana3_0401 at 460.809 kb on - strand, within Shewana3_0401 at 460.960 kb on + strand at 460.983 kb on - strand at 460.995 kb on + strand at 461.037 kb on + strand at 461.200 kb on - strand at 461.200 kb on - strand at 461.200 kb on - strand at 461.365 kb on - strand, within Shewana3_0402 at 461.370 kb on - strand, within Shewana3_0402 at 461.387 kb on - strand, within Shewana3_0402 at 461.418 kb on + strand, within Shewana3_0402 at 461.470 kb on - strand, within Shewana3_0402 at 461.535 kb on + strand, within Shewana3_0402 at 461.566 kb on + strand, within Shewana3_0402 at 461.571 kb on + strand, within Shewana3_0402 at 461.670 kb on + strand, within Shewana3_0402 at 461.765 kb on + strand, within Shewana3_0402 at 461.765 kb on + strand, within Shewana3_0402 at 461.942 kb on + strand at 462.015 kb on - strand at 462.025 kb on + strand at 462.103 kb on + strand at 462.237 kb on + strand, within Shewana3_0403 at 462.505 kb on + strand, within Shewana3_0403 at 462.550 kb on + strand, within Shewana3_0403 at 462.558 kb on - strand, within Shewana3_0403 at 462.630 kb on + strand, within Shewana3_0403 at 462.657 kb on + strand, within Shewana3_0403 at 462.657 kb on + strand, within Shewana3_0403 at 462.663 kb on + strand, within Shewana3_0403 at 462.664 kb on - strand, within Shewana3_0403 at 462.699 kb on - strand, within Shewana3_0403 at 462.707 kb on + strand, within Shewana3_0403 at 462.797 kb on + strand, within Shewana3_0403
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.02 mg/ml remove 460,344 - +1.0 460,368 + Shewana3_0401 0.10 +0.4 460,387 + Shewana3_0401 0.13 -0.7 460,395 - Shewana3_0401 0.14 -0.1 460,395 - Shewana3_0401 0.14 +0.2 460,402 + Shewana3_0401 0.15 +0.1 460,501 + Shewana3_0401 0.29 -0.6 460,514 - Shewana3_0401 0.30 +0.8 460,514 - Shewana3_0401 0.30 +0.2 460,650 - Shewana3_0401 0.49 -0.5 460,729 - Shewana3_0401 0.60 -1.8 460,799 + Shewana3_0401 0.70 -0.5 460,809 - Shewana3_0401 0.71 +0.2 460,960 + +0.2 460,983 - +0.0 460,995 + -0.5 461,037 + -0.3 461,200 - -1.4 461,200 - -1.5 461,200 - -0.8 461,365 - Shewana3_0402 0.21 -0.5 461,370 - Shewana3_0402 0.22 +0.5 461,387 - Shewana3_0402 0.24 +2.0 461,418 + Shewana3_0402 0.29 +0.1 461,470 - Shewana3_0402 0.36 +0.8 461,535 + Shewana3_0402 0.46 -0.5 461,566 + Shewana3_0402 0.51 -0.1 461,571 + Shewana3_0402 0.51 +0.2 461,670 + Shewana3_0402 0.66 +0.0 461,765 + Shewana3_0402 0.80 +0.0 461,765 + Shewana3_0402 0.80 +0.7 461,942 + +0.3 462,015 - -0.2 462,025 + +0.3 462,103 + -0.1 462,237 + Shewana3_0403 0.14 -0.3 462,505 + Shewana3_0403 0.27 -0.1 462,550 + Shewana3_0403 0.29 +0.1 462,558 - Shewana3_0403 0.30 +0.2 462,630 + Shewana3_0403 0.33 -0.2 462,657 + Shewana3_0403 0.35 +0.2 462,657 + Shewana3_0403 0.35 -0.0 462,663 + Shewana3_0403 0.35 +0.4 462,664 - Shewana3_0403 0.35 -0.5 462,699 - Shewana3_0403 0.37 -0.4 462,707 + Shewana3_0403 0.37 -0.5 462,797 + Shewana3_0403 0.42 +0.0
Or see this region's nucleotide sequence