Experiment: A22 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0246 and Shewana3_0247 are separated by 28 nucleotides Shewana3_0247 and Shewana3_0248 overlap by 4 nucleotides Shewana3_0248 and Shewana3_0249 are separated by 135 nucleotides Shewana3_0249 and Shewana3_0250 are separated by 69 nucleotides
Shewana3_0246: Shewana3_0246 - hypothetical protein (RefSeq), at 277,346 to 277,768
_0246
Shewana3_0247: Shewana3_0247 - hypothetical protein (RefSeq), at 277,797 to 278,285
_0247
Shewana3_0248: Shewana3_0248 - LysR family transcriptional regulator (RefSeq), at 278,282 to 279,199
_0248
Shewana3_0249: Shewana3_0249 - short-chain dehydrogenase/reductase SDR (RefSeq), at 279,335 to 280,096
_0249
Shewana3_0250: Shewana3_0250 - aldehyde dehydrogenase (RefSeq), at 280,166 to 281,686
_0250
Position (kb) 278
279
280 Strain fitness (log2 ratio) -1
0
1
2 at 277.603 kb on - strand, within Shewana3_0246 at 277.624 kb on + strand, within Shewana3_0246 at 278.004 kb on - strand, within Shewana3_0247 at 278.085 kb on - strand, within Shewana3_0247 at 278.201 kb on + strand, within Shewana3_0247 at 278.386 kb on + strand, within Shewana3_0248 at 278.485 kb on - strand, within Shewana3_0248 at 278.543 kb on + strand, within Shewana3_0248 at 278.551 kb on - strand, within Shewana3_0248 at 278.686 kb on + strand, within Shewana3_0248 at 278.694 kb on - strand, within Shewana3_0248 at 278.717 kb on - strand, within Shewana3_0248 at 278.822 kb on - strand, within Shewana3_0248 at 278.888 kb on - strand, within Shewana3_0248 at 279.115 kb on + strand at 279.400 kb on + strand at 279.500 kb on + strand, within Shewana3_0249 at 279.647 kb on + strand, within Shewana3_0249 at 279.665 kb on - strand, within Shewana3_0249 at 279.778 kb on - strand, within Shewana3_0249 at 279.977 kb on + strand, within Shewana3_0249 at 279.977 kb on + strand, within Shewana3_0249 at 280.004 kb on + strand, within Shewana3_0249
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.02 mg/ml remove 277,603 - Shewana3_0246 0.61 +0.1 277,624 + Shewana3_0246 0.66 -0.2 278,004 - Shewana3_0247 0.42 +0.0 278,085 - Shewana3_0247 0.59 -0.1 278,201 + Shewana3_0247 0.83 +0.0 278,386 + Shewana3_0248 0.11 +0.2 278,485 - Shewana3_0248 0.22 +0.6 278,543 + Shewana3_0248 0.28 -0.2 278,551 - Shewana3_0248 0.29 +1.5 278,686 + Shewana3_0248 0.44 +0.4 278,694 - Shewana3_0248 0.45 +1.1 278,717 - Shewana3_0248 0.47 +2.1 278,822 - Shewana3_0248 0.59 -0.1 278,888 - Shewana3_0248 0.66 +0.2 279,115 + +0.4 279,400 + +0.0 279,500 + Shewana3_0249 0.22 -0.5 279,647 + Shewana3_0249 0.41 -0.1 279,665 - Shewana3_0249 0.43 +0.1 279,778 - Shewana3_0249 0.58 -0.6 279,977 + Shewana3_0249 0.84 -0.5 279,977 + Shewana3_0249 0.84 -0.0 280,004 + Shewana3_0249 0.88 -0.5
Or see this region's nucleotide sequence