Experiment: L-Glutamic (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5655 and Pf1N1B4_5656 are separated by 11 nucleotides Pf1N1B4_5656 and Pf1N1B4_5657 overlap by 4 nucleotides Pf1N1B4_5657 and Pf1N1B4_5658 are separated by 82 nucleotides
Pf1N1B4_5655 - FIG00955561: hypothetical protein, at 87,457 to 87,663
_5655
Pf1N1B4_5656 - Predicted cobalt transporter CbtA, at 87,675 to 88,382
_5656
Pf1N1B4_5657 - Cobalt-precorrin-3b C17-methyltransferase, at 88,379 to 88,792
_5657
Pf1N1B4_5658 - Cobalt-precorrin-4 C11-methyltransferase (EC 2.1.1.133), at 88,875 to 89,621
_5658
Position (kb) 88
89 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 87.581 kb on - strand at 87.787 kb on - strand, within Pf1N1B4_5656 at 87.986 kb on + strand at 87.986 kb on + strand, within Pf1N1B4_5656 at 88.372 kb on - strand at 88.884 kb on + strand at 88.885 kb on - strand at 88.968 kb on + strand, within Pf1N1B4_5658 at 88.968 kb on + strand, within Pf1N1B4_5658 at 88.968 kb on + strand, within Pf1N1B4_5658 at 88.968 kb on + strand, within Pf1N1B4_5658 at 88.968 kb on + strand, within Pf1N1B4_5658 at 88.969 kb on - strand at 88.969 kb on - strand, within Pf1N1B4_5658 at 88.969 kb on - strand, within Pf1N1B4_5658 at 88.969 kb on - strand, within Pf1N1B4_5658 at 88.969 kb on - strand, within Pf1N1B4_5658 at 88.997 kb on - strand, within Pf1N1B4_5658 at 89.131 kb on - strand, within Pf1N1B4_5658 at 89.586 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamic (C) remove 87,581 - -1.1 87,787 - Pf1N1B4_5656 0.16 -0.5 87,986 + +0.1 87,986 + Pf1N1B4_5656 0.44 -1.7 88,372 - -0.1 88,884 + -0.3 88,885 - -0.1 88,968 + Pf1N1B4_5658 0.12 +0.1 88,968 + Pf1N1B4_5658 0.12 -0.5 88,968 + Pf1N1B4_5658 0.12 +1.1 88,968 + Pf1N1B4_5658 0.12 -2.5 88,968 + Pf1N1B4_5658 0.12 -0.9 88,969 - -1.5 88,969 - Pf1N1B4_5658 0.13 -2.5 88,969 - Pf1N1B4_5658 0.13 -2.1 88,969 - Pf1N1B4_5658 0.13 -1.5 88,969 - Pf1N1B4_5658 0.13 -1.3 88,997 - Pf1N1B4_5658 0.16 -1.9 89,131 - Pf1N1B4_5658 0.34 -2.1 89,586 + -2.8
Or see this region's nucleotide sequence