Experiment: LB, pH10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_618 and Ac3H11_619 are separated by 267 nucleotides Ac3H11_619 and Ac3H11_620 are separated by 293 nucleotides Ac3H11_620 and Ac3H11_621 are separated by 152 nucleotides
Ac3H11_618 - hypothetical protein, at 57,920 to 58,501
_618
Ac3H11_619 - hypothetical protein, at 58,769 to 59,020
_619
Ac3H11_620 - Transcriptional regulator, TetR family, at 59,314 to 59,898
_620
Ac3H11_621 - Thioredoxin reductase (EC 1.8.1.9), at 60,051 to 60,944
_621
Position (kb)
59
60 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 58.941 kb on - strand, within Ac3H11_619 at 59.053 kb on - strand at 59.053 kb on - strand at 59.053 kb on - strand at 59.113 kb on - strand at 59.175 kb on + strand at 59.175 kb on + strand at 59.175 kb on + strand at 59.176 kb on - strand at 59.176 kb on - strand at 59.222 kb on + strand at 59.222 kb on + strand at 59.223 kb on - strand at 59.334 kb on + strand at 59.334 kb on + strand at 59.335 kb on - strand at 59.335 kb on - strand at 59.401 kb on + strand, within Ac3H11_620 at 59.401 kb on + strand, within Ac3H11_620 at 59.401 kb on + strand, within Ac3H11_620 at 59.401 kb on + strand, within Ac3H11_620 at 59.402 kb on - strand, within Ac3H11_620 at 59.402 kb on - strand, within Ac3H11_620 at 59.402 kb on - strand, within Ac3H11_620 at 59.402 kb on - strand, within Ac3H11_620 at 59.426 kb on - strand, within Ac3H11_620 at 59.426 kb on - strand, within Ac3H11_620 at 59.426 kb on - strand, within Ac3H11_620 at 59.508 kb on - strand, within Ac3H11_620 at 59.542 kb on + strand, within Ac3H11_620 at 59.542 kb on + strand, within Ac3H11_620 at 59.592 kb on - strand, within Ac3H11_620 at 59.783 kb on + strand, within Ac3H11_620 at 59.783 kb on + strand, within Ac3H11_620 at 59.784 kb on - strand, within Ac3H11_620 at 59.784 kb on - strand, within Ac3H11_620 at 59.960 kb on + strand at 59.962 kb on + strand at 59.963 kb on - strand at 59.963 kb on - strand at 59.986 kb on + strand at 59.986 kb on + strand at 59.987 kb on - strand at 59.989 kb on - strand at 59.989 kb on - strand at 59.989 kb on - strand at 59.990 kb on + strand at 60.001 kb on - strand at 60.370 kb on - strand, within Ac3H11_621 at 60.370 kb on - strand, within Ac3H11_621 at 60.370 kb on - strand, within Ac3H11_621 at 60.779 kb on + strand, within Ac3H11_621 at 60.840 kb on + strand, within Ac3H11_621 at 60.840 kb on + strand, within Ac3H11_621 at 60.841 kb on - strand, within Ac3H11_621 at 60.841 kb on - strand, within Ac3H11_621
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH10 remove 58,941 - Ac3H11_619 0.68 -1.2 59,053 - +1.8 59,053 - -1.4 59,053 - +0.6 59,113 - -1.1 59,175 + -1.3 59,175 + -0.7 59,175 + -3.6 59,176 - +1.2 59,176 - +2.4 59,222 + +0.4 59,222 + -0.1 59,223 - +1.4 59,334 + -0.2 59,334 + +2.0 59,335 - +0.9 59,335 - +2.7 59,401 + Ac3H11_620 0.15 -0.3 59,401 + Ac3H11_620 0.15 +3.3 59,401 + Ac3H11_620 0.15 -2.4 59,401 + Ac3H11_620 0.15 -0.6 59,402 - Ac3H11_620 0.15 +1.5 59,402 - Ac3H11_620 0.15 +0.8 59,402 - Ac3H11_620 0.15 +1.3 59,402 - Ac3H11_620 0.15 +3.9 59,426 - Ac3H11_620 0.19 +0.7 59,426 - Ac3H11_620 0.19 +1.1 59,426 - Ac3H11_620 0.19 -2.0 59,508 - Ac3H11_620 0.33 -2.1 59,542 + Ac3H11_620 0.39 +2.1 59,542 + Ac3H11_620 0.39 -0.9 59,592 - Ac3H11_620 0.48 -0.4 59,783 + Ac3H11_620 0.80 -1.1 59,783 + Ac3H11_620 0.80 +2.0 59,784 - Ac3H11_620 0.80 +2.0 59,784 - Ac3H11_620 0.80 -2.6 59,960 + -0.0 59,962 + -3.3 59,963 - +1.2 59,963 - +1.8 59,986 + +0.7 59,986 + -2.7 59,987 - -1.4 59,989 - +0.9 59,989 - +1.5 59,989 - -0.4 59,990 + -0.1 60,001 - -1.4 60,370 - Ac3H11_621 0.36 -1.7 60,370 - Ac3H11_621 0.36 -0.5 60,370 - Ac3H11_621 0.36 +1.3 60,779 + Ac3H11_621 0.81 -1.5 60,840 + Ac3H11_621 0.88 +0.5 60,840 + Ac3H11_621 0.88 -0.7 60,841 - Ac3H11_621 0.88 +1.1 60,841 - Ac3H11_621 0.88 +0.4
Or see this region's nucleotide sequence