Experiment: LB, pH10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_4538 and Ac3H11_4539 overlap by 4 nucleotides Ac3H11_4539 and Ac3H11_4540 are separated by 1 nucleotides Ac3H11_4540 and Ac3H11_4541 are separated by 75 nucleotides Ac3H11_4541 and Ac3H11_4542 overlap by 4 nucleotides
Ac3H11_4538 - ATP-binding protein PhnN; Guanylate kinase (EC 2.7.4.8), at 132,683 to 133,297
_4538
Ac3H11_4539 - Protein RcsF, at 133,294 to 134,007
_4539
Ac3H11_4540 - Transcriptional regulator PhnF, at 134,009 to 134,791
_4540
Ac3H11_4541 - PhnG protein, at 134,867 to 135,379
_4541
Ac3H11_4542 - PhnH protein, at 135,376 to 136,017
_4542
Position (kb) 134
135 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 133.268 kb on + strand at 133.269 kb on - strand at 133.275 kb on + strand at 133.275 kb on + strand at 133.276 kb on - strand at 133.276 kb on - strand at 133.827 kb on - strand, within Ac3H11_4539 at 133.976 kb on + strand at 133.976 kb on + strand at 133.986 kb on - strand at 133.986 kb on - strand at 133.986 kb on - strand at 134.114 kb on + strand, within Ac3H11_4540 at 134.115 kb on - strand, within Ac3H11_4540 at 134.115 kb on - strand, within Ac3H11_4540 at 134.209 kb on - strand, within Ac3H11_4540 at 134.302 kb on - strand, within Ac3H11_4540 at 134.526 kb on + strand, within Ac3H11_4540 at 134.526 kb on + strand, within Ac3H11_4540 at 134.798 kb on + strand at 134.798 kb on + strand at 134.799 kb on - strand at 134.842 kb on - strand at 134.844 kb on - strand at 135.153 kb on - strand, within Ac3H11_4541 at 135.153 kb on - strand, within Ac3H11_4541 at 135.356 kb on + strand at 135.356 kb on + strand at 135.356 kb on + strand at 135.495 kb on - strand, within Ac3H11_4542
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH10 remove 133,268 + -1.8 133,269 - +2.1 133,275 + -0.6 133,275 + -1.2 133,276 - -2.3 133,276 - +1.9 133,827 - Ac3H11_4539 0.75 -1.0 133,976 + +0.4 133,976 + +1.5 133,986 - +0.7 133,986 - -2.3 133,986 - -0.3 134,114 + Ac3H11_4540 0.13 +2.2 134,115 - Ac3H11_4540 0.14 +0.3 134,115 - Ac3H11_4540 0.14 -0.9 134,209 - Ac3H11_4540 0.26 +1.2 134,302 - Ac3H11_4540 0.37 -1.6 134,526 + Ac3H11_4540 0.66 -3.4 134,526 + Ac3H11_4540 0.66 +1.0 134,798 + -0.3 134,798 + +0.1 134,799 - -1.3 134,842 - -2.2 134,844 - +1.8 135,153 - Ac3H11_4541 0.56 +2.2 135,153 - Ac3H11_4541 0.56 +0.8 135,356 + +0.3 135,356 + +0.1 135,356 + +0.0 135,495 - Ac3H11_4542 0.19 -0.7
Or see this region's nucleotide sequence