Experiment: LB, pH10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_3517 and Ac3H11_3518 are separated by 110 nucleotides Ac3H11_3518 and Ac3H11_3519 overlap by 17 nucleotides Ac3H11_3519 and Ac3H11_3520 overlap by 16 nucleotides Ac3H11_3520 and Ac3H11_3521 are separated by 31 nucleotides Ac3H11_3521 and Ac3H11_3522 are separated by 131 nucleotides
Ac3H11_3517 - Transcriptional regulator, LysR family, at 62,991 to 63,893
_3517
Ac3H11_3518 - Rrf2-linked NADH-flavin reductase, at 64,004 to 64,645
_3518
Ac3H11_3519 - hypothetical protein, at 64,629 to 64,796
_3519
Ac3H11_3520 - TRAP-type C4-dicarboxylate transport system, large permease component, at 64,781 to 66,061
_3520
Ac3H11_3521 - TRAP-type transport system, small permease component, predicted N-acetylneuraminate transporter, at 66,093 to 66,662
_3521
Ac3H11_3522 - Fumarylacetoacetate hydrolase family protein, at 66,794 to 68,011
_3522
Position (kb) 64
65
66
67 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 63.899 kb on - strand at 63.943 kb on - strand at 63.965 kb on + strand at 63.977 kb on - strand at 64.211 kb on + strand, within Ac3H11_3518 at 64.211 kb on + strand, within Ac3H11_3518 at 64.212 kb on - strand, within Ac3H11_3518 at 64.212 kb on - strand, within Ac3H11_3518 at 64.212 kb on - strand, within Ac3H11_3518 at 64.698 kb on + strand, within Ac3H11_3519 at 64.717 kb on - strand, within Ac3H11_3519 at 64.717 kb on - strand, within Ac3H11_3519 at 64.996 kb on - strand, within Ac3H11_3520 at 65.493 kb on + strand, within Ac3H11_3520 at 65.998 kb on - strand at 66.050 kb on - strand at 66.386 kb on - strand, within Ac3H11_3521 at 66.556 kb on + strand, within Ac3H11_3521 at 66.556 kb on + strand, within Ac3H11_3521 at 66.557 kb on - strand, within Ac3H11_3521 at 66.693 kb on + strand at 66.694 kb on - strand at 66.796 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH10 remove 63,899 - +0.2 63,943 - +2.4 63,965 + -1.3 63,977 - -2.2 64,211 + Ac3H11_3518 0.32 -0.9 64,211 + Ac3H11_3518 0.32 +2.5 64,212 - Ac3H11_3518 0.32 +2.6 64,212 - Ac3H11_3518 0.32 +2.4 64,212 - Ac3H11_3518 0.32 +0.6 64,698 + Ac3H11_3519 0.41 -1.1 64,717 - Ac3H11_3519 0.52 +0.6 64,717 - Ac3H11_3519 0.52 -0.9 64,996 - Ac3H11_3520 0.17 +0.3 65,493 + Ac3H11_3520 0.56 -0.3 65,998 - -1.2 66,050 - +3.5 66,386 - Ac3H11_3521 0.51 -0.3 66,556 + Ac3H11_3521 0.81 +1.1 66,556 + Ac3H11_3521 0.81 -1.0 66,557 - Ac3H11_3521 0.81 +0.9 66,693 + +2.1 66,694 - +0.1 66,796 - -0.7
Or see this region's nucleotide sequence