Experiment: LB, pH10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1007 and Ac3H11_1008 are separated by 54 nucleotides Ac3H11_1008 and Ac3H11_1009 overlap by 4 nucleotides Ac3H11_1009 and Ac3H11_1010 are separated by 95 nucleotides Ac3H11_1010 and Ac3H11_1011 overlap by 4 nucleotides
Ac3H11_1007 - Hydantoin racemase (EC 5.1.99.-), at 131,361 to 132,107
_1007
Ac3H11_1008 - Prevent host death protein, Phd antitoxin, at 132,162 to 132,386
_1008
Ac3H11_1009 - Death on curing protein, Doc toxin, at 132,383 to 132,778
_1009
Ac3H11_1010 - Endonuclease III (EC 4.2.99.18), at 132,874 to 133,518
_1010
Ac3H11_1011 - Transcriptional regulator, LysR family, at 133,515 to 134,483
_1011
Position (kb)
132
133 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 131.490 kb on + strand, within Ac3H11_1007 at 131.490 kb on + strand, within Ac3H11_1007 at 131.490 kb on + strand, within Ac3H11_1007 at 131.490 kb on + strand, within Ac3H11_1007 at 131.491 kb on - strand, within Ac3H11_1007 at 131.491 kb on - strand, within Ac3H11_1007 at 131.491 kb on - strand, within Ac3H11_1007 at 131.972 kb on + strand, within Ac3H11_1007 at 131.973 kb on - strand, within Ac3H11_1007 at 132.128 kb on - strand at 132.400 kb on + strand at 132.401 kb on - strand at 132.401 kb on - strand at 132.401 kb on - strand at 132.401 kb on - strand at 132.521 kb on + strand, within Ac3H11_1009 at 132.522 kb on - strand, within Ac3H11_1009 at 132.569 kb on + strand, within Ac3H11_1009 at 132.569 kb on + strand, within Ac3H11_1009 at 132.570 kb on - strand, within Ac3H11_1009 at 132.828 kb on + strand at 132.959 kb on + strand, within Ac3H11_1010 at 132.959 kb on + strand, within Ac3H11_1010 at 132.959 kb on + strand, within Ac3H11_1010 at 132.959 kb on + strand, within Ac3H11_1010 at 132.959 kb on + strand, within Ac3H11_1010 at 132.960 kb on - strand, within Ac3H11_1010 at 132.960 kb on - strand, within Ac3H11_1010 at 132.960 kb on - strand, within Ac3H11_1010 at 132.960 kb on - strand, within Ac3H11_1010 at 132.960 kb on - strand, within Ac3H11_1010 at 133.001 kb on + strand, within Ac3H11_1010 at 133.289 kb on + strand, within Ac3H11_1010 at 133.289 kb on + strand, within Ac3H11_1010 at 133.289 kb on + strand, within Ac3H11_1010 at 133.289 kb on + strand, within Ac3H11_1010 at 133.289 kb on + strand, within Ac3H11_1010 at 133.290 kb on - strand, within Ac3H11_1010 at 133.436 kb on + strand, within Ac3H11_1010 at 133.437 kb on - strand, within Ac3H11_1010 at 133.437 kb on - strand, within Ac3H11_1010 at 133.437 kb on - strand, within Ac3H11_1010
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH10 remove 131,490 + Ac3H11_1007 0.17 -0.6 131,490 + Ac3H11_1007 0.17 +0.1 131,490 + Ac3H11_1007 0.17 -0.8 131,490 + Ac3H11_1007 0.17 -0.2 131,491 - Ac3H11_1007 0.17 -0.7 131,491 - Ac3H11_1007 0.17 +1.3 131,491 - Ac3H11_1007 0.17 -0.2 131,972 + Ac3H11_1007 0.82 -0.3 131,973 - Ac3H11_1007 0.82 -0.3 132,128 - -0.6 132,400 + +0.5 132,401 - -0.6 132,401 - -0.9 132,401 - +1.7 132,401 - +0.0 132,521 + Ac3H11_1009 0.35 -1.4 132,522 - Ac3H11_1009 0.35 -0.5 132,569 + Ac3H11_1009 0.47 +0.1 132,569 + Ac3H11_1009 0.47 -2.6 132,570 - Ac3H11_1009 0.47 -3.5 132,828 + -1.8 132,959 + Ac3H11_1010 0.13 -2.5 132,959 + Ac3H11_1010 0.13 -0.3 132,959 + Ac3H11_1010 0.13 +0.4 132,959 + Ac3H11_1010 0.13 -0.5 132,959 + Ac3H11_1010 0.13 -0.6 132,960 - Ac3H11_1010 0.13 +0.1 132,960 - Ac3H11_1010 0.13 +0.9 132,960 - Ac3H11_1010 0.13 -1.0 132,960 - Ac3H11_1010 0.13 -0.8 132,960 - Ac3H11_1010 0.13 -3.0 133,001 + Ac3H11_1010 0.20 -0.6 133,289 + Ac3H11_1010 0.64 -0.5 133,289 + Ac3H11_1010 0.64 -0.7 133,289 + Ac3H11_1010 0.64 -0.5 133,289 + Ac3H11_1010 0.64 -1.0 133,289 + Ac3H11_1010 0.64 -0.2 133,290 - Ac3H11_1010 0.64 +0.3 133,436 + Ac3H11_1010 0.87 -0.9 133,437 - Ac3H11_1010 0.87 +0.5 133,437 - Ac3H11_1010 0.87 -1.2 133,437 - Ac3H11_1010 0.87 +0.2
Or see this region's nucleotide sequence