Experiment: A22 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt napG and napH overlap by 4 nucleotides napH and Shewana3_3433 overlap by 4 nucleotides Shewana3_3433 and Shewana3_3434 are separated by 249 nucleotides Shewana3_3434 and Shewana3_3435 are separated by 94 nucleotides
Shewana3_3431: napG - quinol dehydrogenase periplasmic component (RefSeq), at 4,108,372 to 4,109,106
napG
Shewana3_3432: napH - quinol dehydrogenase membrane component (RefSeq), at 4,109,103 to 4,110,086
napH
Shewana3_3433: Shewana3_3433 - periplasmic nitrate reductase subunit NapB (RefSeq), at 4,110,083 to 4,110,514
_3433
Shewana3_3434: Shewana3_3434 - hypothetical protein (RefSeq), at 4,110,764 to 4,110,916
_3434
Shewana3_3435: Shewana3_3435 - LysR family transcriptional regulator (RefSeq), at 4,111,011 to 4,111,898
_3435
Position (kb)
4110
4111 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4109.503 kb on + strand, within napH at 4109.584 kb on - strand, within napH at 4109.623 kb on - strand, within napH at 4109.728 kb on - strand, within napH at 4109.728 kb on - strand, within napH at 4109.814 kb on + strand, within napH at 4109.833 kb on + strand, within napH at 4109.859 kb on + strand, within napH at 4109.929 kb on + strand, within napH at 4110.159 kb on - strand, within Shewana3_3433 at 4110.519 kb on + strand at 4110.646 kb on + strand at 4110.933 kb on - strand at 4110.948 kb on + strand at 4111.049 kb on - strand at 4111.149 kb on - strand, within Shewana3_3435 at 4111.187 kb on + strand, within Shewana3_3435 at 4111.187 kb on + strand, within Shewana3_3435 at 4111.440 kb on - strand, within Shewana3_3435 at 4111.481 kb on + strand, within Shewana3_3435
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.008 mg/ml remove 4,109,503 + napH Shewana3_3432 0.41 -0.2 4,109,584 - napH Shewana3_3432 0.49 -0.8 4,109,623 - napH Shewana3_3432 0.53 -0.1 4,109,728 - napH Shewana3_3432 0.64 -1.0 4,109,728 - napH Shewana3_3432 0.64 -0.3 4,109,814 + napH Shewana3_3432 0.72 -0.0 4,109,833 + napH Shewana3_3432 0.74 +0.5 4,109,859 + napH Shewana3_3432 0.77 -0.1 4,109,929 + napH Shewana3_3432 0.84 -0.1 4,110,159 - Shewana3_3433 0.18 +1.0 4,110,519 + -0.6 4,110,646 + -0.1 4,110,933 - -0.2 4,110,948 + +1.4 4,111,049 - +0.1 4,111,149 - Shewana3_3435 0.16 -2.1 4,111,187 + Shewana3_3435 0.20 -0.4 4,111,187 + Shewana3_3435 0.20 -0.3 4,111,440 - Shewana3_3435 0.48 +0.9 4,111,481 + Shewana3_3435 0.53 +1.9
Or see this region's nucleotide sequence