Experiment: A22 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1981 and Shewana3_1982 are separated by 72 nucleotides Shewana3_1982 and Shewana3_1983 are separated by 59 nucleotides Shewana3_1983 and Shewana3_1984 are separated by 217 nucleotides
Shewana3_1981: Shewana3_1981 - orotidine 5'-phosphate decarboxylase (RefSeq), at 2,359,529 to 2,360,224
_1981
Shewana3_1982: Shewana3_1982 - short chain dehydrogenase (RefSeq), at 2,360,297 to 2,361,124
_1982
Shewana3_1983: Shewana3_1983 - hypothetical protein (RefSeq), at 2,361,184 to 2,361,726
_1983
Shewana3_1984: Shewana3_1984 - acyl-CoA dehydrogenase domain-containing protein (RefSeq), at 2,361,944 to 2,363,701
_1984
Position (kb)
2361
2362 Strain fitness (log2 ratio)
-2
-1
0
1 at 2360.475 kb on - strand, within Shewana3_1982 at 2360.536 kb on - strand, within Shewana3_1982 at 2360.969 kb on + strand, within Shewana3_1982 at 2361.043 kb on - strand at 2361.210 kb on + strand at 2361.210 kb on + strand at 2361.210 kb on + strand at 2361.462 kb on + strand, within Shewana3_1983 at 2361.550 kb on - strand, within Shewana3_1983 at 2361.568 kb on + strand, within Shewana3_1983 at 2362.100 kb on + strand at 2362.112 kb on + strand at 2362.313 kb on + strand, within Shewana3_1984 at 2362.509 kb on + strand, within Shewana3_1984
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.008 mg/ml remove 2,360,475 - Shewana3_1982 0.21 +0.6 2,360,536 - Shewana3_1982 0.29 +0.6 2,360,969 + Shewana3_1982 0.81 +0.4 2,361,043 - +0.6 2,361,210 + +0.6 2,361,210 + -0.4 2,361,210 + +1.0 2,361,462 + Shewana3_1983 0.51 +0.4 2,361,550 - Shewana3_1983 0.67 +0.6 2,361,568 + Shewana3_1983 0.71 +0.7 2,362,100 + +1.2 2,362,112 + -1.0 2,362,313 + Shewana3_1984 0.21 -1.0 2,362,509 + Shewana3_1984 0.32 -2.0
Or see this region's nucleotide sequence