Experiment: A22 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0004 and trmE are separated by 103 nucleotides trmE and Shewana3_0006 are separated by 103 nucleotides
Shewana3_0004: Shewana3_0004 - TGF-beta receptor, type I/II extracellular region (RefSeq), at 3,725 to 5,272
_0004
Shewana3_0005: trmE - tRNA modification GTPase TrmE (RefSeq), at 5,376 to 6,737
trmE
Shewana3_0006: Shewana3_0006 - protein translocase subunit yidC (RefSeq), at 6,841 to 8,466
_0006
Position (kb) 5
6
7 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4.438 kb on - strand, within Shewana3_0004 at 4.479 kb on - strand, within Shewana3_0004 at 4.492 kb on - strand, within Shewana3_0004 at 4.512 kb on + strand, within Shewana3_0004 at 4.578 kb on - strand, within Shewana3_0004 at 4.619 kb on - strand, within Shewana3_0004 at 4.719 kb on - strand, within Shewana3_0004 at 4.719 kb on - strand, within Shewana3_0004 at 4.786 kb on + strand, within Shewana3_0004 at 4.816 kb on + strand, within Shewana3_0004 at 4.851 kb on - strand, within Shewana3_0004 at 4.851 kb on - strand, within Shewana3_0004 at 4.957 kb on - strand, within Shewana3_0004 at 5.160 kb on + strand at 5.231 kb on + strand at 5.433 kb on - strand at 5.536 kb on - strand, within trmE at 5.557 kb on + strand, within trmE at 5.610 kb on - strand, within trmE at 6.126 kb on + strand, within trmE at 6.126 kb on + strand, within trmE at 6.129 kb on - strand at 6.357 kb on + strand, within trmE at 6.569 kb on - strand, within trmE at 6.604 kb on + strand at 6.612 kb on + strand at 6.725 kb on + strand at 6.745 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.008 mg/ml remove 4,438 - Shewana3_0004 0.46 -0.2 4,479 - Shewana3_0004 0.49 +0.1 4,492 - Shewana3_0004 0.50 -0.8 4,512 + Shewana3_0004 0.51 -0.2 4,578 - Shewana3_0004 0.55 -0.3 4,619 - Shewana3_0004 0.58 +0.0 4,719 - Shewana3_0004 0.64 +1.0 4,719 - Shewana3_0004 0.64 -1.0 4,786 + Shewana3_0004 0.69 -0.4 4,816 + Shewana3_0004 0.70 -0.1 4,851 - Shewana3_0004 0.73 -0.8 4,851 - Shewana3_0004 0.73 +0.9 4,957 - Shewana3_0004 0.80 -0.6 5,160 + -0.8 5,231 + +0.1 5,433 - -0.0 5,536 - trmE Shewana3_0005 0.12 -1.1 5,557 + trmE Shewana3_0005 0.13 -1.0 5,610 - trmE Shewana3_0005 0.17 -0.3 6,126 + trmE Shewana3_0005 0.55 -0.5 6,126 + trmE Shewana3_0005 0.55 -2.6 6,129 - +0.9 6,357 + trmE Shewana3_0005 0.72 -0.4 6,569 - trmE Shewana3_0005 0.88 -3.1 6,604 + -0.9 6,612 + -0.8 6,725 + -1.4 6,745 - -0.0
Or see this region's nucleotide sequence