Experiment: A22 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4227 and Shewana3_4228 are separated by 651 nucleotides Shewana3_4228 and Shewana3_4229 are separated by 72 nucleotides Shewana3_4229 and Shewana3_4230 are separated by 128 nucleotides
Shewana3_4227: Shewana3_4227 - initiator RepB protein (RefSeq), at 100,875 to 102,038
_4227
Shewana3_4228: Shewana3_4228 - hypothetical protein (RefSeq), at 102,690 to 103,265
_4228
Shewana3_4229: Shewana3_4229 - single-strand binding protein (RefSeq), at 103,338 to 103,865
_4229
Shewana3_4230: Shewana3_4230 - hypothetical protein (RefSeq), at 103,994 to 105,601
_4230
Position (kb) 102
103
104 Strain fitness (log2 ratio) -1
0
1 at 102.711 kb on - strand at 102.773 kb on + strand, within Shewana3_4228 at 102.805 kb on + strand, within Shewana3_4228 at 102.813 kb on - strand, within Shewana3_4228 at 103.053 kb on + strand, within Shewana3_4228 at 103.053 kb on + strand, within Shewana3_4228 at 103.056 kb on - strand, within Shewana3_4228 at 103.148 kb on - strand, within Shewana3_4228 at 103.321 kb on + strand at 103.329 kb on - strand at 103.466 kb on - strand, within Shewana3_4229 at 103.632 kb on - strand, within Shewana3_4229 at 103.682 kb on + strand, within Shewana3_4229 at 103.859 kb on + strand at 103.866 kb on + strand at 103.867 kb on - strand at 103.912 kb on + strand at 103.925 kb on + strand at 104.223 kb on + strand, within Shewana3_4230
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.008 mg/ml remove 102,711 - +1.1 102,773 + Shewana3_4228 0.14 -0.3 102,805 + Shewana3_4228 0.20 -0.6 102,813 - Shewana3_4228 0.21 +1.1 103,053 + Shewana3_4228 0.63 -1.0 103,053 + Shewana3_4228 0.63 -1.0 103,056 - Shewana3_4228 0.64 +0.7 103,148 - Shewana3_4228 0.80 +0.0 103,321 + -0.1 103,329 - +1.4 103,466 - Shewana3_4229 0.24 +0.1 103,632 - Shewana3_4229 0.56 -0.1 103,682 + Shewana3_4229 0.65 +0.1 103,859 + -0.8 103,866 + -0.6 103,867 - +0.1 103,912 + -0.4 103,925 + -0.7 104,223 + Shewana3_4230 0.14 -0.7
Or see this region's nucleotide sequence