Experiment: L-Histidine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_823 and Pf1N1B4_824 are separated by 2 nucleotides Pf1N1B4_824 and Pf1N1B4_825 are separated by 150 nucleotides Pf1N1B4_825 and Pf1N1B4_826 overlap by 4 nucleotides
Pf1N1B4_823 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 945,479 to 945,871
_823
Pf1N1B4_824 - POSSIBLE MEMBRANE PROTEIN, at 945,874 to 946,518
_824
Pf1N1B4_825 - tRNA (uridine-5-oxyacetic acid methyl ester) 34 synthase, at 946,669 to 947,412
_825
Pf1N1B4_826 - tRNA (5-methoxyuridine) 34 synthase, at 947,409 to 948,365
_826
Position (kb) 946
947
948 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 945.704 kb on + strand, within Pf1N1B4_823 at 945.803 kb on + strand, within Pf1N1B4_823 at 945.803 kb on + strand, within Pf1N1B4_823 at 945.803 kb on + strand, within Pf1N1B4_823 at 945.804 kb on - strand, within Pf1N1B4_823 at 946.135 kb on + strand, within Pf1N1B4_824 at 946.135 kb on + strand, within Pf1N1B4_824 at 946.412 kb on + strand, within Pf1N1B4_824 at 946.413 kb on - strand, within Pf1N1B4_824 at 946.413 kb on - strand, within Pf1N1B4_824 at 946.435 kb on + strand, within Pf1N1B4_824 at 946.435 kb on + strand, within Pf1N1B4_824 at 946.435 kb on + strand, within Pf1N1B4_824 at 946.436 kb on - strand, within Pf1N1B4_824 at 946.436 kb on - strand, within Pf1N1B4_824 at 946.436 kb on - strand, within Pf1N1B4_824 at 946.453 kb on + strand, within Pf1N1B4_824 at 946.453 kb on + strand, within Pf1N1B4_824 at 946.454 kb on - strand at 946.852 kb on + strand, within Pf1N1B4_825 at 946.852 kb on + strand, within Pf1N1B4_825 at 946.938 kb on + strand, within Pf1N1B4_825 at 946.972 kb on + strand, within Pf1N1B4_825 at 946.973 kb on - strand, within Pf1N1B4_825 at 947.251 kb on + strand, within Pf1N1B4_825 at 947.383 kb on - strand at 947.799 kb on + strand, within Pf1N1B4_826 at 947.802 kb on + strand at 947.802 kb on + strand, within Pf1N1B4_826 at 947.802 kb on + strand, within Pf1N1B4_826 at 947.802 kb on + strand, within Pf1N1B4_826 at 947.802 kb on + strand, within Pf1N1B4_826 at 947.802 kb on + strand, within Pf1N1B4_826 at 947.803 kb on - strand, within Pf1N1B4_826 at 947.803 kb on - strand, within Pf1N1B4_826 at 947.803 kb on - strand, within Pf1N1B4_826 at 947.803 kb on - strand, within Pf1N1B4_826 at 947.803 kb on - strand, within Pf1N1B4_826 at 947.803 kb on - strand, within Pf1N1B4_826 at 947.895 kb on + strand, within Pf1N1B4_826 at 947.895 kb on + strand at 947.896 kb on - strand, within Pf1N1B4_826 at 947.995 kb on - strand, within Pf1N1B4_826 at 948.267 kb on + strand at 948.267 kb on + strand, within Pf1N1B4_826 at 948.267 kb on + strand, within Pf1N1B4_826 at 948.267 kb on + strand, within Pf1N1B4_826 at 948.267 kb on + strand, within Pf1N1B4_826 at 948.268 kb on - strand, within Pf1N1B4_826 at 948.363 kb on + strand at 948.363 kb on + strand at 948.363 kb on + strand at 948.388 kb on + strand at 948.388 kb on + strand at 948.389 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (C) remove 945,704 + Pf1N1B4_823 0.57 -1.6 945,803 + Pf1N1B4_823 0.82 -1.3 945,803 + Pf1N1B4_823 0.82 +0.3 945,803 + Pf1N1B4_823 0.82 +1.1 945,804 - Pf1N1B4_823 0.83 -2.3 946,135 + Pf1N1B4_824 0.40 +0.6 946,135 + Pf1N1B4_824 0.40 -2.0 946,412 + Pf1N1B4_824 0.83 -1.3 946,413 - Pf1N1B4_824 0.84 +1.0 946,413 - Pf1N1B4_824 0.84 -0.3 946,435 + Pf1N1B4_824 0.87 -1.6 946,435 + Pf1N1B4_824 0.87 +0.6 946,435 + Pf1N1B4_824 0.87 -1.0 946,436 - Pf1N1B4_824 0.87 -0.2 946,436 - Pf1N1B4_824 0.87 +1.0 946,436 - Pf1N1B4_824 0.87 -1.6 946,453 + Pf1N1B4_824 0.90 -0.4 946,453 + Pf1N1B4_824 0.90 -1.3 946,454 - -1.0 946,852 + Pf1N1B4_825 0.25 -2.0 946,852 + Pf1N1B4_825 0.25 -0.0 946,938 + Pf1N1B4_825 0.36 -1.6 946,972 + Pf1N1B4_825 0.41 -0.3 946,973 - Pf1N1B4_825 0.41 -1.6 947,251 + Pf1N1B4_825 0.78 -0.6 947,383 - +2.0 947,799 + Pf1N1B4_826 0.41 -1.0 947,802 + +2.0 947,802 + Pf1N1B4_826 0.41 -2.3 947,802 + Pf1N1B4_826 0.41 -0.6 947,802 + Pf1N1B4_826 0.41 -0.9 947,802 + Pf1N1B4_826 0.41 -0.6 947,802 + Pf1N1B4_826 0.41 +1.0 947,803 - Pf1N1B4_826 0.41 +0.6 947,803 - Pf1N1B4_826 0.41 -1.6 947,803 - Pf1N1B4_826 0.41 +0.3 947,803 - Pf1N1B4_826 0.41 -0.0 947,803 - Pf1N1B4_826 0.41 -1.0 947,803 - Pf1N1B4_826 0.41 +1.0 947,895 + Pf1N1B4_826 0.51 -0.4 947,895 + -1.0 947,896 - Pf1N1B4_826 0.51 -3.0 947,995 - Pf1N1B4_826 0.61 +0.6 948,267 + +2.0 948,267 + Pf1N1B4_826 0.90 -0.0 948,267 + Pf1N1B4_826 0.90 -0.0 948,267 + Pf1N1B4_826 0.90 -1.0 948,267 + Pf1N1B4_826 0.90 -1.0 948,268 - Pf1N1B4_826 0.90 +1.0 948,363 + -3.6 948,363 + -1.0 948,363 + -0.2 948,388 + -0.6 948,388 + -0.0 948,389 - -1.0
Or see this region's nucleotide sequence