Experiment: L-Histidine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5679 and Pf1N1B4_5680 overlap by 4 nucleotides Pf1N1B4_5680 and Pf1N1B4_5681 are separated by 7 nucleotides Pf1N1B4_5681 and Pf1N1B4_5682 overlap by 1 nucleotides
Pf1N1B4_5679 - DNA polymerase III alpha subunit (EC 2.7.7.7), at 116,795 to 119,872
_5679
Pf1N1B4_5680 - DNA polymerase-like protein PA0670, at 119,869 to 121,284
_5680
Pf1N1B4_5681 - RecA/RadA recombinase, at 121,292 to 121,909
_5681
Pf1N1B4_5682 - SOS-response repressor and protease LexA (EC 3.4.21.88), at 121,909 to 122,526
_5682
Position (kb)
119
120
121
122 Strain fitness (log2 ratio)
-1
0
1 at 119.418 kb on - strand, within Pf1N1B4_5679 at 119.418 kb on - strand, within Pf1N1B4_5679 at 119.418 kb on - strand, within Pf1N1B4_5679 at 119.557 kb on - strand, within Pf1N1B4_5679 at 119.667 kb on - strand at 119.960 kb on + strand at 119.961 kb on - strand at 119.964 kb on - strand at 120.098 kb on - strand at 120.098 kb on - strand, within Pf1N1B4_5680 at 120.098 kb on - strand, within Pf1N1B4_5680 at 120.256 kb on + strand, within Pf1N1B4_5680 at 120.594 kb on - strand, within Pf1N1B4_5680 at 120.594 kb on - strand, within Pf1N1B4_5680 at 120.616 kb on + strand, within Pf1N1B4_5680 at 120.675 kb on + strand, within Pf1N1B4_5680 at 120.675 kb on + strand, within Pf1N1B4_5680 at 120.676 kb on - strand, within Pf1N1B4_5680 at 120.818 kb on - strand at 120.818 kb on - strand, within Pf1N1B4_5680 at 121.071 kb on - strand, within Pf1N1B4_5680 at 121.986 kb on - strand, within Pf1N1B4_5682 at 122.002 kb on + strand at 122.134 kb on - strand, within Pf1N1B4_5682 at 122.174 kb on + strand, within Pf1N1B4_5682 at 122.175 kb on - strand, within Pf1N1B4_5682 at 122.175 kb on - strand, within Pf1N1B4_5682
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (C) remove 119,418 - Pf1N1B4_5679 0.85 +0.1 119,418 - Pf1N1B4_5679 0.85 +0.9 119,418 - Pf1N1B4_5679 0.85 +0.5 119,557 - Pf1N1B4_5679 0.90 +0.9 119,667 - -0.1 119,960 + -0.1 119,961 - -0.5 119,964 - -1.1 120,098 - -0.7 120,098 - Pf1N1B4_5680 0.16 +0.5 120,098 - Pf1N1B4_5680 0.16 -0.1 120,256 + Pf1N1B4_5680 0.27 -0.4 120,594 - Pf1N1B4_5680 0.51 +0.5 120,594 - Pf1N1B4_5680 0.51 +1.5 120,616 + Pf1N1B4_5680 0.53 -0.1 120,675 + Pf1N1B4_5680 0.57 +0.5 120,675 + Pf1N1B4_5680 0.57 -1.1 120,676 - Pf1N1B4_5680 0.57 +0.9 120,818 - -0.1 120,818 - Pf1N1B4_5680 0.67 +1.0 121,071 - Pf1N1B4_5680 0.85 -0.6 121,986 - Pf1N1B4_5682 0.12 -0.4 122,002 + -0.7 122,134 - Pf1N1B4_5682 0.36 +0.7 122,174 + Pf1N1B4_5682 0.43 -0.4 122,175 - Pf1N1B4_5682 0.43 +1.3 122,175 - Pf1N1B4_5682 0.43 -0.1
Or see this region's nucleotide sequence