Experiment: L-Histidine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4926 and Pf1N1B4_4927 overlap by 4 nucleotides Pf1N1B4_4927 and Pf1N1B4_4928 overlap by 19 nucleotides Pf1N1B4_4928 and Pf1N1B4_4929 overlap by 4 nucleotides
Pf1N1B4_4926 - ATPase components of various ABC-type transport systems, contain duplicated ATPase, at 5,459,110 to 5,460,951
_4926
Pf1N1B4_4927 - Hypothetical, similar to sarcosine oxidase alpha subunit, 2Fe-2S domain, at 5,460,948 to 5,461,256
_4927
Pf1N1B4_4928 - Ferredoxin reductase, at 5,461,238 to 5,462,629
_4928
Pf1N1B4_4929 - Opine oxidase subunit B, at 5,462,626 to 5,463,795
_4929
Position (kb)
5461
5462
5463 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5460.280 kb on + strand, within Pf1N1B4_4926 at 5460.280 kb on + strand, within Pf1N1B4_4926 at 5460.280 kb on + strand, within Pf1N1B4_4926 at 5460.281 kb on - strand, within Pf1N1B4_4926 at 5460.281 kb on - strand, within Pf1N1B4_4926 at 5460.281 kb on - strand, within Pf1N1B4_4926 at 5460.326 kb on + strand at 5460.327 kb on - strand, within Pf1N1B4_4926 at 5460.327 kb on - strand, within Pf1N1B4_4926 at 5460.538 kb on + strand, within Pf1N1B4_4926 at 5460.601 kb on + strand at 5460.665 kb on - strand, within Pf1N1B4_4926 at 5460.665 kb on - strand, within Pf1N1B4_4926 at 5460.665 kb on - strand, within Pf1N1B4_4926 at 5461.379 kb on + strand, within Pf1N1B4_4928 at 5461.402 kb on + strand, within Pf1N1B4_4928 at 5461.412 kb on + strand at 5461.425 kb on - strand, within Pf1N1B4_4928 at 5461.649 kb on + strand, within Pf1N1B4_4928 at 5461.649 kb on + strand, within Pf1N1B4_4928 at 5461.649 kb on + strand, within Pf1N1B4_4928 at 5461.649 kb on + strand, within Pf1N1B4_4928 at 5461.650 kb on - strand, within Pf1N1B4_4928 at 5461.650 kb on - strand at 5461.739 kb on + strand, within Pf1N1B4_4928 at 5461.740 kb on - strand, within Pf1N1B4_4928 at 5461.757 kb on + strand, within Pf1N1B4_4928 at 5461.935 kb on + strand, within Pf1N1B4_4928 at 5462.077 kb on + strand, within Pf1N1B4_4928 at 5462.154 kb on - strand at 5462.154 kb on - strand, within Pf1N1B4_4928 at 5462.634 kb on - strand at 5463.325 kb on + strand, within Pf1N1B4_4929 at 5463.353 kb on - strand, within Pf1N1B4_4929 at 5463.353 kb on - strand, within Pf1N1B4_4929 at 5463.379 kb on + strand, within Pf1N1B4_4929 at 5463.379 kb on + strand, within Pf1N1B4_4929 at 5463.460 kb on + strand at 5463.566 kb on - strand, within Pf1N1B4_4929 at 5463.619 kb on + strand, within Pf1N1B4_4929 at 5463.620 kb on - strand, within Pf1N1B4_4929 at 5463.623 kb on - strand, within Pf1N1B4_4929
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (C) remove 5,460,280 + Pf1N1B4_4926 0.64 +0.5 5,460,280 + Pf1N1B4_4926 0.64 +0.2 5,460,280 + Pf1N1B4_4926 0.64 -1.0 5,460,281 - Pf1N1B4_4926 0.64 -3.6 5,460,281 - Pf1N1B4_4926 0.64 -0.6 5,460,281 - Pf1N1B4_4926 0.64 -0.5 5,460,326 + -0.3 5,460,327 - Pf1N1B4_4926 0.66 -1.8 5,460,327 - Pf1N1B4_4926 0.66 -1.8 5,460,538 + Pf1N1B4_4926 0.78 -0.8 5,460,601 + -0.3 5,460,665 - Pf1N1B4_4926 0.84 -1.3 5,460,665 - Pf1N1B4_4926 0.84 -0.3 5,460,665 - Pf1N1B4_4926 0.84 -1.8 5,461,379 + Pf1N1B4_4928 0.10 -0.8 5,461,402 + Pf1N1B4_4928 0.12 +0.7 5,461,412 + +0.7 5,461,425 - Pf1N1B4_4928 0.13 -0.1 5,461,649 + Pf1N1B4_4928 0.30 -0.3 5,461,649 + Pf1N1B4_4928 0.30 -1.6 5,461,649 + Pf1N1B4_4928 0.30 -0.8 5,461,649 + Pf1N1B4_4928 0.30 +0.2 5,461,650 - Pf1N1B4_4928 0.30 -0.3 5,461,650 - -0.3 5,461,739 + Pf1N1B4_4928 0.36 +1.3 5,461,740 - Pf1N1B4_4928 0.36 +0.7 5,461,757 + Pf1N1B4_4928 0.37 +0.5 5,461,935 + Pf1N1B4_4928 0.50 +2.1 5,462,077 + Pf1N1B4_4928 0.60 +1.6 5,462,154 - -0.3 5,462,154 - Pf1N1B4_4928 0.66 +0.7 5,462,634 - -0.8 5,463,325 + Pf1N1B4_4929 0.60 +0.7 5,463,353 - Pf1N1B4_4929 0.62 +0.1 5,463,353 - Pf1N1B4_4929 0.62 +0.2 5,463,379 + Pf1N1B4_4929 0.64 +1.3 5,463,379 + Pf1N1B4_4929 0.64 +0.3 5,463,460 + -2.3 5,463,566 - Pf1N1B4_4929 0.80 +0.5 5,463,619 + Pf1N1B4_4929 0.85 +0.3 5,463,620 - Pf1N1B4_4929 0.85 +0.3 5,463,623 - Pf1N1B4_4929 0.85 -1.3
Or see this region's nucleotide sequence