Experiment: L-Histidine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4608 and Pf1N1B4_4609 overlap by 1 nucleotides Pf1N1B4_4609 and Pf1N1B4_4610 overlap by 31 nucleotides Pf1N1B4_4610 and Pf1N1B4_4611 overlap by 4 nucleotides Pf1N1B4_4611 and Pf1N1B4_4612 are separated by 30 nucleotides Pf1N1B4_4612 and Pf1N1B4_4613 overlap by 4 nucleotides
Pf1N1B4_4608 - NAD kinase (EC 2.7.1.23), at 5,079,704 to 5,079,934
_4608
Pf1N1B4_4609 - Diadenosine tetraphosphatase and related serine/threonine protein phosphatases, at 5,079,934 to 5,080,398
_4609
Pf1N1B4_4610 - Diadenosine tetraphosphatase and related serine/threonine protein phosphatases, at 5,080,368 to 5,080,904
_4610
Pf1N1B4_4611 - GlpG protein (membrane protein of glp regulon), at 5,080,901 to 5,081,773
_4611
Pf1N1B4_4612 - FIG00954117: hypothetical protein, at 5,081,804 to 5,082,064
_4612
Pf1N1B4_4613 - FIG00953934: hypothetical protein, at 5,082,061 to 5,082,891
_4613
Position (kb)
5080
5081
5082 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5079.951 kb on + strand at 5079.956 kb on - strand at 5080.243 kb on - strand, within Pf1N1B4_4609 at 5080.329 kb on + strand, within Pf1N1B4_4609 at 5080.701 kb on + strand, within Pf1N1B4_4610 at 5080.737 kb on + strand, within Pf1N1B4_4610 at 5080.859 kb on + strand at 5080.920 kb on + strand at 5080.920 kb on + strand at 5080.920 kb on + strand at 5080.920 kb on + strand at 5080.921 kb on - strand at 5081.019 kb on + strand, within Pf1N1B4_4611 at 5081.136 kb on + strand, within Pf1N1B4_4611 at 5081.477 kb on + strand, within Pf1N1B4_4611 at 5081.477 kb on + strand, within Pf1N1B4_4611 at 5081.477 kb on + strand, within Pf1N1B4_4611 at 5081.477 kb on + strand, within Pf1N1B4_4611 at 5081.477 kb on + strand, within Pf1N1B4_4611 at 5081.744 kb on + strand at 5081.744 kb on + strand at 5081.745 kb on - strand at 5081.772 kb on - strand at 5081.776 kb on + strand at 5081.776 kb on + strand at 5081.777 kb on - strand at 5081.777 kb on - strand at 5081.777 kb on - strand at 5081.849 kb on + strand, within Pf1N1B4_4612 at 5081.849 kb on + strand, within Pf1N1B4_4612 at 5081.850 kb on - strand, within Pf1N1B4_4612 at 5081.850 kb on - strand, within Pf1N1B4_4612 at 5081.850 kb on - strand, within Pf1N1B4_4612 at 5081.987 kb on + strand at 5081.988 kb on - strand, within Pf1N1B4_4612 at 5082.197 kb on - strand, within Pf1N1B4_4613 at 5082.329 kb on - strand, within Pf1N1B4_4613 at 5082.329 kb on - strand, within Pf1N1B4_4613 at 5082.578 kb on + strand, within Pf1N1B4_4613
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (C) remove 5,079,951 + +0.2 5,079,956 - +0.5 5,080,243 - Pf1N1B4_4609 0.66 -1.2 5,080,329 + Pf1N1B4_4609 0.85 -0.2 5,080,701 + Pf1N1B4_4610 0.62 -0.2 5,080,737 + Pf1N1B4_4610 0.69 -1.2 5,080,859 + -1.9 5,080,920 + -1.2 5,080,920 + +0.1 5,080,920 + -0.5 5,080,920 + +0.4 5,080,921 - -2.0 5,081,019 + Pf1N1B4_4611 0.14 -1.2 5,081,136 + Pf1N1B4_4611 0.27 +0.4 5,081,477 + Pf1N1B4_4611 0.66 -2.2 5,081,477 + Pf1N1B4_4611 0.66 -2.5 5,081,477 + Pf1N1B4_4611 0.66 +2.4 5,081,477 + Pf1N1B4_4611 0.66 -0.2 5,081,477 + Pf1N1B4_4611 0.66 -1.2 5,081,744 + +0.4 5,081,744 + -0.5 5,081,745 - -0.2 5,081,772 - -1.8 5,081,776 + -0.2 5,081,776 + -0.5 5,081,777 - +0.4 5,081,777 - +0.5 5,081,777 - -0.2 5,081,849 + Pf1N1B4_4612 0.17 +1.0 5,081,849 + Pf1N1B4_4612 0.17 -2.0 5,081,850 - Pf1N1B4_4612 0.18 +0.8 5,081,850 - Pf1N1B4_4612 0.18 -0.2 5,081,850 - Pf1N1B4_4612 0.18 +0.5 5,081,987 + +0.8 5,081,988 - Pf1N1B4_4612 0.70 +0.0 5,082,197 - Pf1N1B4_4613 0.16 -0.6 5,082,329 - Pf1N1B4_4613 0.32 -0.9 5,082,329 - Pf1N1B4_4613 0.32 +1.4 5,082,578 + Pf1N1B4_4613 0.62 +2.8
Or see this region's nucleotide sequence