Experiment: L-Histidine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4542 and Pf1N1B4_4543 are separated by 2 nucleotides Pf1N1B4_4543 and Pf1N1B4_4544 are separated by 502 nucleotides Pf1N1B4_4544 and Pf1N1B4_4545 are separated by 88 nucleotides Pf1N1B4_4545 and Pf1N1B4_4546 overlap by 2 nucleotides
Pf1N1B4_4542 - IcmF-related protein, at 5,009,265 to 5,012,888
_4542
Pf1N1B4_4543 - IcmF-related protein, at 5,012,891 to 5,013,040
_4543
Pf1N1B4_4544 - Uncharacterized protein ImpA, at 5,013,543 to 5,014,181
_4544
Pf1N1B4_4545 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 5,014,270 to 5,014,758
_4545
Pf1N1B4_4546 - hypothetical protein, at 5,014,757 to 5,015,215
_4546
Position (kb) 5013
5014
5015 Strain fitness (log2 ratio) -1
0
1 at 5012.799 kb on + strand at 5012.821 kb on + strand at 5012.872 kb on - strand at 5013.790 kb on - strand, within Pf1N1B4_4544 at 5014.030 kb on - strand, within Pf1N1B4_4544 at 5014.065 kb on + strand, within Pf1N1B4_4544 at 5014.179 kb on + strand at 5015.115 kb on + strand, within Pf1N1B4_4546
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (C) remove 5,012,799 + +0.4 5,012,821 + -0.2 5,012,872 - +0.8 5,013,790 - Pf1N1B4_4544 0.39 -1.2 5,014,030 - Pf1N1B4_4544 0.76 -0.8 5,014,065 + Pf1N1B4_4544 0.82 -1.2 5,014,179 + +0.2 5,015,115 + Pf1N1B4_4546 0.78 +0.4
Or see this region's nucleotide sequence