Experiment: L-Histidine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3500 and Pf1N1B4_3501 are separated by 199 nucleotides Pf1N1B4_3501 and Pf1N1B4_3502 are separated by 23 nucleotides Pf1N1B4_3502 and Pf1N1B4_3503 overlap by 21 nucleotides Pf1N1B4_3503 and Pf1N1B4_3504 are separated by 1 nucleotides
Pf1N1B4_3500 - D-lactate dehydrogenase (EC 1.1.1.28), at 3,904,118 to 3,905,107
_3500
Pf1N1B4_3501 - BarA sensory histidine kinase (= VarS = GacS), at 3,905,307 to 3,908,057
_3501
Pf1N1B4_3502 - hypothetical protein, at 3,908,081 to 3,908,347
_3502
Pf1N1B4_3503 - Two-component response regulator PfeR, enterobactin, at 3,908,327 to 3,908,995
_3503
Pf1N1B4_3504 - Two-component sensor histidine kinase PfeS, enterobactin, at 3,908,997 to 3,910,412
_3504
Position (kb)
3905
3906
3907
3908
3909 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3904.330 kb on - strand, within Pf1N1B4_3500 at 3904.330 kb on - strand, within Pf1N1B4_3500 at 3904.421 kb on + strand, within Pf1N1B4_3500 at 3904.421 kb on + strand, within Pf1N1B4_3500 at 3904.421 kb on + strand, within Pf1N1B4_3500 at 3904.421 kb on + strand, within Pf1N1B4_3500 at 3904.422 kb on - strand, within Pf1N1B4_3500 at 3904.737 kb on + strand, within Pf1N1B4_3500 at 3904.737 kb on + strand, within Pf1N1B4_3500 at 3904.737 kb on + strand, within Pf1N1B4_3500 at 3904.737 kb on + strand, within Pf1N1B4_3500 at 3904.738 kb on - strand at 3904.809 kb on + strand, within Pf1N1B4_3500 at 3904.809 kb on + strand, within Pf1N1B4_3500 at 3904.810 kb on - strand, within Pf1N1B4_3500 at 3904.929 kb on - strand, within Pf1N1B4_3500 at 3905.076 kb on + strand at 3905.077 kb on - strand at 3905.125 kb on + strand at 3905.125 kb on + strand at 3905.126 kb on - strand at 3905.126 kb on - strand at 3905.132 kb on - strand at 3905.219 kb on - strand at 3905.273 kb on + strand at 3905.295 kb on + strand at 3905.607 kb on + strand, within Pf1N1B4_3501 at 3905.607 kb on + strand at 3905.608 kb on - strand, within Pf1N1B4_3501 at 3905.608 kb on - strand, within Pf1N1B4_3501 at 3905.608 kb on - strand, within Pf1N1B4_3501 at 3905.752 kb on + strand, within Pf1N1B4_3501 at 3905.752 kb on + strand, within Pf1N1B4_3501 at 3905.753 kb on - strand, within Pf1N1B4_3501 at 3905.753 kb on - strand, within Pf1N1B4_3501 at 3905.753 kb on - strand, within Pf1N1B4_3501 at 3905.753 kb on - strand at 3906.266 kb on + strand, within Pf1N1B4_3501 at 3906.266 kb on + strand at 3906.266 kb on + strand, within Pf1N1B4_3501 at 3906.266 kb on + strand, within Pf1N1B4_3501 at 3906.267 kb on - strand at 3906.267 kb on - strand, within Pf1N1B4_3501 at 3906.267 kb on - strand, within Pf1N1B4_3501 at 3906.766 kb on + strand, within Pf1N1B4_3501 at 3906.767 kb on - strand, within Pf1N1B4_3501 at 3906.767 kb on - strand, within Pf1N1B4_3501 at 3906.767 kb on - strand, within Pf1N1B4_3501 at 3906.767 kb on - strand, within Pf1N1B4_3501 at 3906.767 kb on - strand, within Pf1N1B4_3501 at 3906.767 kb on - strand, within Pf1N1B4_3501 at 3906.767 kb on - strand, within Pf1N1B4_3501 at 3906.767 kb on - strand at 3906.891 kb on - strand, within Pf1N1B4_3501 at 3907.093 kb on + strand, within Pf1N1B4_3501 at 3907.093 kb on + strand, within Pf1N1B4_3501 at 3907.094 kb on - strand, within Pf1N1B4_3501 at 3907.973 kb on - strand at 3908.037 kb on - strand at 3908.037 kb on - strand at 3908.037 kb on - strand at 3908.037 kb on - strand at 3908.082 kb on + strand at 3908.339 kb on + strand at 3908.339 kb on + strand at 3908.339 kb on + strand at 3908.339 kb on + strand at 3908.340 kb on - strand at 3908.585 kb on + strand, within Pf1N1B4_3503 at 3908.786 kb on - strand, within Pf1N1B4_3503 at 3908.786 kb on - strand, within Pf1N1B4_3503 at 3908.786 kb on - strand, within Pf1N1B4_3503 at 3908.786 kb on - strand, within Pf1N1B4_3503 at 3908.811 kb on + strand, within Pf1N1B4_3503 at 3908.811 kb on + strand, within Pf1N1B4_3503 at 3908.811 kb on + strand, within Pf1N1B4_3503 at 3908.811 kb on + strand at 3908.812 kb on - strand at 3908.812 kb on - strand, within Pf1N1B4_3503 at 3908.812 kb on - strand, within Pf1N1B4_3503 at 3908.812 kb on - strand, within Pf1N1B4_3503 at 3908.841 kb on + strand, within Pf1N1B4_3503 at 3908.841 kb on + strand, within Pf1N1B4_3503 at 3908.945 kb on - strand at 3908.963 kb on + strand at 3908.967 kb on + strand at 3908.967 kb on + strand at 3908.967 kb on + strand at 3908.967 kb on + strand at 3908.968 kb on - strand at 3908.968 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (C) remove 3,904,330 - Pf1N1B4_3500 0.21 +1.5 3,904,330 - Pf1N1B4_3500 0.21 +1.5 3,904,421 + Pf1N1B4_3500 0.31 +0.5 3,904,421 + Pf1N1B4_3500 0.31 -0.9 3,904,421 + Pf1N1B4_3500 0.31 -1.7 3,904,421 + Pf1N1B4_3500 0.31 -0.8 3,904,422 - Pf1N1B4_3500 0.31 +0.9 3,904,737 + Pf1N1B4_3500 0.63 +0.1 3,904,737 + Pf1N1B4_3500 0.63 -1.7 3,904,737 + Pf1N1B4_3500 0.63 -0.1 3,904,737 + Pf1N1B4_3500 0.63 +0.5 3,904,738 - +0.9 3,904,809 + Pf1N1B4_3500 0.70 -0.9 3,904,809 + Pf1N1B4_3500 0.70 -0.7 3,904,810 - Pf1N1B4_3500 0.70 -0.2 3,904,929 - Pf1N1B4_3500 0.82 +0.5 3,905,076 + +1.3 3,905,077 - -0.7 3,905,125 + -1.5 3,905,125 + -0.5 3,905,126 - -2.1 3,905,126 - -0.9 3,905,132 - +0.7 3,905,219 - +1.5 3,905,273 + +0.9 3,905,295 + -1.0 3,905,607 + Pf1N1B4_3501 0.11 +0.9 3,905,607 + -0.1 3,905,608 - Pf1N1B4_3501 0.11 -2.5 3,905,608 - Pf1N1B4_3501 0.11 -0.1 3,905,608 - Pf1N1B4_3501 0.11 -2.7 3,905,752 + Pf1N1B4_3501 0.16 -0.1 3,905,752 + Pf1N1B4_3501 0.16 -2.1 3,905,753 - Pf1N1B4_3501 0.16 -1.7 3,905,753 - Pf1N1B4_3501 0.16 -1.1 3,905,753 - Pf1N1B4_3501 0.16 -1.1 3,905,753 - -1.7 3,906,266 + Pf1N1B4_3501 0.35 -0.1 3,906,266 + -1.1 3,906,266 + Pf1N1B4_3501 0.35 +0.5 3,906,266 + Pf1N1B4_3501 0.35 -0.5 3,906,267 - +0.9 3,906,267 - Pf1N1B4_3501 0.35 -0.7 3,906,267 - Pf1N1B4_3501 0.35 -3.1 3,906,766 + Pf1N1B4_3501 0.53 -2.5 3,906,767 - Pf1N1B4_3501 0.53 -0.7 3,906,767 - Pf1N1B4_3501 0.53 -2.1 3,906,767 - Pf1N1B4_3501 0.53 -2.1 3,906,767 - Pf1N1B4_3501 0.53 -2.7 3,906,767 - Pf1N1B4_3501 0.53 -1.1 3,906,767 - Pf1N1B4_3501 0.53 -0.1 3,906,767 - Pf1N1B4_3501 0.53 -0.5 3,906,767 - -1.1 3,906,891 - Pf1N1B4_3501 0.58 -2.1 3,907,093 + Pf1N1B4_3501 0.65 -1.4 3,907,093 + Pf1N1B4_3501 0.65 +0.9 3,907,094 - Pf1N1B4_3501 0.65 -2.1 3,907,973 - -2.1 3,908,037 - -2.5 3,908,037 - -0.1 3,908,037 - -0.1 3,908,037 - -1.7 3,908,082 + -0.7 3,908,339 + -0.9 3,908,339 + +1.5 3,908,339 + -0.5 3,908,339 + -1.7 3,908,340 - +0.9 3,908,585 + Pf1N1B4_3503 0.39 -0.1 3,908,786 - Pf1N1B4_3503 0.69 -2.1 3,908,786 - Pf1N1B4_3503 0.69 -3.3 3,908,786 - Pf1N1B4_3503 0.69 -0.1 3,908,786 - Pf1N1B4_3503 0.69 -2.3 3,908,811 + Pf1N1B4_3503 0.72 -0.0 3,908,811 + Pf1N1B4_3503 0.72 +0.9 3,908,811 + Pf1N1B4_3503 0.72 +0.4 3,908,811 + -1.7 3,908,812 - -0.1 3,908,812 - Pf1N1B4_3503 0.72 +0.9 3,908,812 - Pf1N1B4_3503 0.72 +0.1 3,908,812 - Pf1N1B4_3503 0.72 +0.9 3,908,841 + Pf1N1B4_3503 0.77 -1.1 3,908,841 + Pf1N1B4_3503 0.77 -0.1 3,908,945 - -1.1 3,908,963 + -0.6 3,908,967 + -0.1 3,908,967 + -0.1 3,908,967 + -2.1 3,908,967 + -0.4 3,908,968 - +0.2 3,908,968 - -0.1
Or see this region's nucleotide sequence