Experiment: L-Histidine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1672 and Pf1N1B4_1673 are separated by 91 nucleotides Pf1N1B4_1673 and Pf1N1B4_1674 overlap by 4 nucleotides Pf1N1B4_1674 and Pf1N1B4_1675 are separated by 124 nucleotides
Pf1N1B4_1672 - Hydrogen peroxide-inducible genes activator, at 1,915,286 to 1,916,173
_1672
Pf1N1B4_1673 - Formate dehydrogenase chain D (EC 1.2.1.2), at 1,916,265 to 1,917,104
_1673
Pf1N1B4_1674 - Putative formate dehydrogenase oxidoreductase protein, at 1,917,101 to 1,919,449
_1674
Pf1N1B4_1675 - Transcriptional regulator, at 1,919,574 to 1,920,494
_1675
Position (kb)
1917
1918
1919
1920 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1916.104 kb on + strand at 1916.147 kb on + strand at 1916.147 kb on + strand at 1916.147 kb on + strand at 1916.147 kb on + strand at 1916.148 kb on - strand at 1916.148 kb on - strand at 1916.148 kb on - strand at 1916.148 kb on - strand at 1916.148 kb on - strand at 1916.148 kb on - strand at 1916.148 kb on - strand at 1916.186 kb on - strand at 1916.200 kb on - strand at 1916.202 kb on + strand at 1916.202 kb on + strand at 1916.202 kb on + strand at 1916.202 kb on + strand at 1916.233 kb on - strand at 1916.233 kb on - strand at 1916.233 kb on - strand at 1916.239 kb on - strand at 1916.239 kb on - strand at 1916.239 kb on - strand at 1916.334 kb on + strand at 1916.334 kb on + strand at 1916.334 kb on + strand at 1916.334 kb on + strand at 1916.334 kb on + strand at 1916.334 kb on + strand at 1916.334 kb on + strand at 1916.335 kb on - strand at 1916.335 kb on - strand at 1916.340 kb on + strand at 1916.355 kb on + strand, within Pf1N1B4_1673 at 1916.355 kb on + strand, within Pf1N1B4_1673 at 1916.355 kb on + strand, within Pf1N1B4_1673 at 1916.355 kb on + strand, within Pf1N1B4_1673 at 1916.355 kb on + strand, within Pf1N1B4_1673 at 1916.355 kb on + strand, within Pf1N1B4_1673 at 1916.356 kb on - strand, within Pf1N1B4_1673 at 1916.356 kb on - strand, within Pf1N1B4_1673 at 1916.356 kb on - strand, within Pf1N1B4_1673 at 1916.356 kb on - strand, within Pf1N1B4_1673 at 1916.356 kb on - strand, within Pf1N1B4_1673 at 1916.356 kb on - strand, within Pf1N1B4_1673 at 1916.409 kb on + strand, within Pf1N1B4_1673 at 1916.409 kb on + strand, within Pf1N1B4_1673 at 1916.409 kb on + strand, within Pf1N1B4_1673 at 1916.409 kb on + strand, within Pf1N1B4_1673 at 1916.409 kb on + strand, within Pf1N1B4_1673 at 1916.410 kb on - strand, within Pf1N1B4_1673 at 1916.410 kb on - strand, within Pf1N1B4_1673 at 1916.410 kb on - strand, within Pf1N1B4_1673 at 1916.410 kb on - strand, within Pf1N1B4_1673 at 1916.410 kb on - strand, within Pf1N1B4_1673 at 1916.513 kb on + strand, within Pf1N1B4_1673 at 1916.517 kb on + strand, within Pf1N1B4_1673 at 1916.517 kb on + strand, within Pf1N1B4_1673 at 1916.517 kb on + strand, within Pf1N1B4_1673 at 1916.518 kb on - strand, within Pf1N1B4_1673 at 1916.518 kb on - strand, within Pf1N1B4_1673 at 1916.518 kb on - strand, within Pf1N1B4_1673 at 1916.518 kb on - strand, within Pf1N1B4_1673 at 1916.518 kb on - strand, within Pf1N1B4_1673 at 1916.554 kb on - strand, within Pf1N1B4_1673 at 1916.554 kb on - strand, within Pf1N1B4_1673 at 1916.554 kb on - strand, within Pf1N1B4_1673 at 1916.554 kb on - strand, within Pf1N1B4_1673 at 1916.780 kb on - strand, within Pf1N1B4_1673 at 1916.986 kb on + strand, within Pf1N1B4_1673 at 1917.075 kb on + strand at 1917.076 kb on - strand at 1917.076 kb on - strand at 1917.152 kb on + strand at 1917.152 kb on + strand at 1917.152 kb on + strand at 1917.153 kb on - strand at 1917.153 kb on - strand at 1917.240 kb on + strand at 1917.241 kb on - strand at 1917.340 kb on + strand, within Pf1N1B4_1674 at 1917.400 kb on + strand, within Pf1N1B4_1674 at 1917.400 kb on + strand, within Pf1N1B4_1674 at 1917.400 kb on + strand, within Pf1N1B4_1674 at 1917.401 kb on - strand, within Pf1N1B4_1674 at 1917.401 kb on - strand, within Pf1N1B4_1674 at 1917.401 kb on - strand, within Pf1N1B4_1674 at 1917.401 kb on - strand, within Pf1N1B4_1674 at 1917.443 kb on + strand, within Pf1N1B4_1674 at 1917.443 kb on + strand, within Pf1N1B4_1674 at 1917.444 kb on - strand, within Pf1N1B4_1674 at 1917.444 kb on - strand, within Pf1N1B4_1674 at 1917.473 kb on + strand, within Pf1N1B4_1674 at 1917.473 kb on + strand, within Pf1N1B4_1674 at 1917.473 kb on + strand, within Pf1N1B4_1674 at 1917.473 kb on + strand, within Pf1N1B4_1674 at 1917.473 kb on + strand, within Pf1N1B4_1674 at 1917.473 kb on + strand, within Pf1N1B4_1674 at 1917.473 kb on + strand, within Pf1N1B4_1674 at 1917.473 kb on + strand, within Pf1N1B4_1674 at 1917.474 kb on - strand, within Pf1N1B4_1674 at 1917.474 kb on - strand, within Pf1N1B4_1674 at 1917.474 kb on - strand, within Pf1N1B4_1674 at 1917.474 kb on - strand, within Pf1N1B4_1674 at 1917.474 kb on - strand, within Pf1N1B4_1674 at 1917.474 kb on - strand, within Pf1N1B4_1674 at 1917.578 kb on + strand, within Pf1N1B4_1674 at 1917.578 kb on + strand, within Pf1N1B4_1674 at 1917.578 kb on + strand, within Pf1N1B4_1674 at 1917.579 kb on - strand, within Pf1N1B4_1674 at 1917.579 kb on - strand, within Pf1N1B4_1674 at 1917.618 kb on - strand, within Pf1N1B4_1674 at 1917.954 kb on - strand, within Pf1N1B4_1674 at 1917.954 kb on - strand, within Pf1N1B4_1674 at 1918.103 kb on + strand, within Pf1N1B4_1674 at 1918.103 kb on + strand, within Pf1N1B4_1674 at 1918.103 kb on + strand, within Pf1N1B4_1674 at 1918.104 kb on - strand, within Pf1N1B4_1674 at 1918.193 kb on + strand, within Pf1N1B4_1674 at 1918.193 kb on + strand, within Pf1N1B4_1674 at 1918.193 kb on + strand, within Pf1N1B4_1674 at 1918.193 kb on + strand, within Pf1N1B4_1674 at 1918.193 kb on + strand, within Pf1N1B4_1674 at 1918.193 kb on + strand, within Pf1N1B4_1674 at 1918.194 kb on - strand, within Pf1N1B4_1674 at 1918.194 kb on - strand, within Pf1N1B4_1674 at 1918.555 kb on + strand, within Pf1N1B4_1674 at 1918.556 kb on - strand, within Pf1N1B4_1674 at 1918.634 kb on - strand, within Pf1N1B4_1674 at 1918.674 kb on + strand, within Pf1N1B4_1674 at 1918.859 kb on + strand, within Pf1N1B4_1674 at 1918.860 kb on - strand, within Pf1N1B4_1674 at 1918.943 kb on + strand, within Pf1N1B4_1674 at 1918.944 kb on - strand, within Pf1N1B4_1674 at 1919.028 kb on + strand, within Pf1N1B4_1674 at 1919.028 kb on + strand, within Pf1N1B4_1674 at 1919.028 kb on + strand, within Pf1N1B4_1674 at 1919.028 kb on + strand, within Pf1N1B4_1674 at 1919.028 kb on + strand, within Pf1N1B4_1674 at 1919.028 kb on + strand, within Pf1N1B4_1674 at 1919.028 kb on + strand, within Pf1N1B4_1674 at 1919.028 kb on + strand, within Pf1N1B4_1674 at 1919.028 kb on + strand, within Pf1N1B4_1674 at 1919.028 kb on + strand, within Pf1N1B4_1674 at 1919.028 kb on + strand, within Pf1N1B4_1674 at 1919.029 kb on - strand, within Pf1N1B4_1674 at 1919.029 kb on - strand, within Pf1N1B4_1674 at 1919.029 kb on - strand, within Pf1N1B4_1674 at 1919.029 kb on - strand, within Pf1N1B4_1674 at 1919.029 kb on - strand, within Pf1N1B4_1674 at 1919.029 kb on - strand, within Pf1N1B4_1674 at 1919.029 kb on - strand, within Pf1N1B4_1674 at 1919.029 kb on - strand, within Pf1N1B4_1674 at 1919.029 kb on - strand, within Pf1N1B4_1674 at 1919.029 kb on - strand, within Pf1N1B4_1674 at 1919.029 kb on - strand, within Pf1N1B4_1674 at 1919.029 kb on - strand, within Pf1N1B4_1674 at 1919.102 kb on + strand at 1919.102 kb on + strand, within Pf1N1B4_1674 at 1919.102 kb on + strand, within Pf1N1B4_1674 at 1919.103 kb on - strand, within Pf1N1B4_1674 at 1919.103 kb on - strand, within Pf1N1B4_1674 at 1919.103 kb on - strand, within Pf1N1B4_1674 at 1919.117 kb on + strand, within Pf1N1B4_1674 at 1919.117 kb on + strand at 1919.118 kb on - strand, within Pf1N1B4_1674 at 1919.118 kb on - strand, within Pf1N1B4_1674 at 1919.118 kb on - strand, within Pf1N1B4_1674 at 1919.315 kb on + strand at 1919.315 kb on + strand at 1919.315 kb on + strand at 1919.316 kb on - strand at 1919.316 kb on - strand at 1919.316 kb on - strand at 1919.318 kb on + strand at 1919.371 kb on + strand at 1919.371 kb on + strand at 1919.372 kb on - strand at 1919.505 kb on + strand at 1919.505 kb on + strand at 1919.505 kb on + strand at 1919.911 kb on + strand, within Pf1N1B4_1675 at 1919.911 kb on + strand, within Pf1N1B4_1675 at 1919.911 kb on + strand, within Pf1N1B4_1675 at 1919.912 kb on - strand, within Pf1N1B4_1675 at 1919.912 kb on - strand, within Pf1N1B4_1675 at 1919.912 kb on - strand, within Pf1N1B4_1675 at 1919.912 kb on - strand, within Pf1N1B4_1675
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (C) remove 1,916,104 + -2.0 1,916,147 + +0.0 1,916,147 + +0.6 1,916,147 + +1.4 1,916,147 + -1.0 1,916,148 - -1.5 1,916,148 - -0.5 1,916,148 - +0.0 1,916,148 - -1.0 1,916,148 - -0.5 1,916,148 - -1.0 1,916,148 - -0.4 1,916,186 - +0.6 1,916,200 - -2.5 1,916,202 + -1.0 1,916,202 + -1.5 1,916,202 + +0.0 1,916,202 + +0.1 1,916,233 - +0.5 1,916,233 - -0.6 1,916,233 - +0.0 1,916,239 - -1.5 1,916,239 - -0.4 1,916,239 - +0.0 1,916,334 + +0.0 1,916,334 + -1.5 1,916,334 + -1.0 1,916,334 + -1.4 1,916,334 + -1.3 1,916,334 + +0.0 1,916,334 + -1.7 1,916,335 - -2.4 1,916,335 - +1.0 1,916,340 + -0.5 1,916,355 + Pf1N1B4_1673 0.11 -0.7 1,916,355 + Pf1N1B4_1673 0.11 -0.4 1,916,355 + Pf1N1B4_1673 0.11 -1.0 1,916,355 + Pf1N1B4_1673 0.11 -2.3 1,916,355 + Pf1N1B4_1673 0.11 -1.8 1,916,355 + Pf1N1B4_1673 0.11 -1.2 1,916,356 - Pf1N1B4_1673 0.11 -2.8 1,916,356 - Pf1N1B4_1673 0.11 -1.5 1,916,356 - Pf1N1B4_1673 0.11 -2.2 1,916,356 - Pf1N1B4_1673 0.11 -0.7 1,916,356 - Pf1N1B4_1673 0.11 +0.6 1,916,356 - Pf1N1B4_1673 0.11 -1.4 1,916,409 + Pf1N1B4_1673 0.17 -1.2 1,916,409 + Pf1N1B4_1673 0.17 -1.5 1,916,409 + Pf1N1B4_1673 0.17 -2.3 1,916,409 + Pf1N1B4_1673 0.17 +0.0 1,916,409 + Pf1N1B4_1673 0.17 +0.0 1,916,410 - Pf1N1B4_1673 0.17 +0.0 1,916,410 - Pf1N1B4_1673 0.17 -2.5 1,916,410 - Pf1N1B4_1673 0.17 -1.3 1,916,410 - Pf1N1B4_1673 0.17 +0.0 1,916,410 - Pf1N1B4_1673 0.17 -0.7 1,916,513 + Pf1N1B4_1673 0.30 +0.3 1,916,517 + Pf1N1B4_1673 0.30 -1.5 1,916,517 + Pf1N1B4_1673 0.30 +0.0 1,916,517 + Pf1N1B4_1673 0.30 +0.0 1,916,518 - Pf1N1B4_1673 0.30 +1.0 1,916,518 - Pf1N1B4_1673 0.30 -1.3 1,916,518 - Pf1N1B4_1673 0.30 -1.5 1,916,518 - Pf1N1B4_1673 0.30 -1.5 1,916,518 - Pf1N1B4_1673 0.30 -2.8 1,916,554 - Pf1N1B4_1673 0.34 -1.3 1,916,554 - Pf1N1B4_1673 0.34 +0.6 1,916,554 - Pf1N1B4_1673 0.34 -0.5 1,916,554 - Pf1N1B4_1673 0.34 -1.4 1,916,780 - Pf1N1B4_1673 0.61 -2.3 1,916,986 + Pf1N1B4_1673 0.86 -2.4 1,917,075 + -1.5 1,917,076 - +1.4 1,917,076 - -2.0 1,917,152 + +0.0 1,917,152 + -1.4 1,917,152 + -2.3 1,917,153 - +0.6 1,917,153 - +1.6 1,917,240 + +1.0 1,917,241 - +1.0 1,917,340 + Pf1N1B4_1674 0.10 -0.8 1,917,400 + Pf1N1B4_1674 0.13 +0.0 1,917,400 + Pf1N1B4_1674 0.13 -1.0 1,917,400 + Pf1N1B4_1674 0.13 -0.4 1,917,401 - Pf1N1B4_1674 0.13 -2.0 1,917,401 - Pf1N1B4_1674 0.13 -2.5 1,917,401 - Pf1N1B4_1674 0.13 +0.0 1,917,401 - Pf1N1B4_1674 0.13 +0.0 1,917,443 + Pf1N1B4_1674 0.15 -1.0 1,917,443 + Pf1N1B4_1674 0.15 -0.4 1,917,444 - Pf1N1B4_1674 0.15 +0.0 1,917,444 - Pf1N1B4_1674 0.15 -1.5 1,917,473 + Pf1N1B4_1674 0.16 +0.0 1,917,473 + Pf1N1B4_1674 0.16 -1.8 1,917,473 + Pf1N1B4_1674 0.16 -1.4 1,917,473 + Pf1N1B4_1674 0.16 -1.0 1,917,473 + Pf1N1B4_1674 0.16 -1.0 1,917,473 + Pf1N1B4_1674 0.16 -0.4 1,917,473 + Pf1N1B4_1674 0.16 -0.5 1,917,473 + Pf1N1B4_1674 0.16 -1.2 1,917,474 - Pf1N1B4_1674 0.16 -0.4 1,917,474 - Pf1N1B4_1674 0.16 +2.0 1,917,474 - Pf1N1B4_1674 0.16 -0.4 1,917,474 - Pf1N1B4_1674 0.16 -0.7 1,917,474 - Pf1N1B4_1674 0.16 -1.0 1,917,474 - Pf1N1B4_1674 0.16 -0.8 1,917,578 + Pf1N1B4_1674 0.20 -0.8 1,917,578 + Pf1N1B4_1674 0.20 -1.3 1,917,578 + Pf1N1B4_1674 0.20 -0.8 1,917,579 - Pf1N1B4_1674 0.20 -1.5 1,917,579 - Pf1N1B4_1674 0.20 -1.0 1,917,618 - Pf1N1B4_1674 0.22 -0.2 1,917,954 - Pf1N1B4_1674 0.36 -2.8 1,917,954 - Pf1N1B4_1674 0.36 -1.1 1,918,103 + Pf1N1B4_1674 0.43 +0.0 1,918,103 + Pf1N1B4_1674 0.43 -0.9 1,918,103 + Pf1N1B4_1674 0.43 +1.6 1,918,104 - Pf1N1B4_1674 0.43 -1.1 1,918,193 + Pf1N1B4_1674 0.46 -1.5 1,918,193 + Pf1N1B4_1674 0.46 -2.5 1,918,193 + Pf1N1B4_1674 0.46 +1.0 1,918,193 + Pf1N1B4_1674 0.46 -1.8 1,918,193 + Pf1N1B4_1674 0.46 -1.5 1,918,193 + Pf1N1B4_1674 0.46 -2.0 1,918,194 - Pf1N1B4_1674 0.47 -1.3 1,918,194 - Pf1N1B4_1674 0.47 -3.3 1,918,555 + Pf1N1B4_1674 0.62 -2.3 1,918,556 - Pf1N1B4_1674 0.62 -1.3 1,918,634 - Pf1N1B4_1674 0.65 -0.8 1,918,674 + Pf1N1B4_1674 0.67 -1.0 1,918,859 + Pf1N1B4_1674 0.75 -2.3 1,918,860 - Pf1N1B4_1674 0.75 -1.0 1,918,943 + Pf1N1B4_1674 0.78 -1.8 1,918,944 - Pf1N1B4_1674 0.78 +1.0 1,919,028 + Pf1N1B4_1674 0.82 -2.0 1,919,028 + Pf1N1B4_1674 0.82 -1.1 1,919,028 + Pf1N1B4_1674 0.82 -0.5 1,919,028 + Pf1N1B4_1674 0.82 -1.5 1,919,028 + Pf1N1B4_1674 0.82 -0.5 1,919,028 + Pf1N1B4_1674 0.82 +0.5 1,919,028 + Pf1N1B4_1674 0.82 +0.0 1,919,028 + Pf1N1B4_1674 0.82 -1.5 1,919,028 + Pf1N1B4_1674 0.82 -1.1 1,919,028 + Pf1N1B4_1674 0.82 -1.0 1,919,028 + Pf1N1B4_1674 0.82 -1.0 1,919,029 - Pf1N1B4_1674 0.82 -1.3 1,919,029 - Pf1N1B4_1674 0.82 +0.6 1,919,029 - Pf1N1B4_1674 0.82 -2.0 1,919,029 - Pf1N1B4_1674 0.82 -0.4 1,919,029 - Pf1N1B4_1674 0.82 -2.0 1,919,029 - Pf1N1B4_1674 0.82 -1.1 1,919,029 - Pf1N1B4_1674 0.82 +0.0 1,919,029 - Pf1N1B4_1674 0.82 -2.0 1,919,029 - Pf1N1B4_1674 0.82 +0.0 1,919,029 - Pf1N1B4_1674 0.82 -2.3 1,919,029 - Pf1N1B4_1674 0.82 -0.7 1,919,029 - Pf1N1B4_1674 0.82 -1.0 1,919,102 + -0.5 1,919,102 + Pf1N1B4_1674 0.85 -1.0 1,919,102 + Pf1N1B4_1674 0.85 +1.0 1,919,103 - Pf1N1B4_1674 0.85 -0.2 1,919,103 - Pf1N1B4_1674 0.85 -2.0 1,919,103 - Pf1N1B4_1674 0.85 -2.1 1,919,117 + Pf1N1B4_1674 0.86 +1.4 1,919,117 + +0.0 1,919,118 - Pf1N1B4_1674 0.86 -1.0 1,919,118 - Pf1N1B4_1674 0.86 -1.0 1,919,118 - Pf1N1B4_1674 0.86 -2.3 1,919,315 + -1.7 1,919,315 + -1.4 1,919,315 + -1.2 1,919,316 - +0.0 1,919,316 - -0.7 1,919,316 - -1.2 1,919,318 + -1.5 1,919,371 + +0.6 1,919,371 + -1.8 1,919,372 - +0.5 1,919,505 + -0.4 1,919,505 + +0.5 1,919,505 + -1.5 1,919,911 + Pf1N1B4_1675 0.37 -1.5 1,919,911 + Pf1N1B4_1675 0.37 +0.6 1,919,911 + Pf1N1B4_1675 0.37 +0.4 1,919,912 - Pf1N1B4_1675 0.37 -0.7 1,919,912 - Pf1N1B4_1675 0.37 -0.6 1,919,912 - Pf1N1B4_1675 0.37 -1.0 1,919,912 - Pf1N1B4_1675 0.37 +0.6
Or see this region's nucleotide sequence