Experiment: L-Histidine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1587 and Pf1N1B4_1588 are separated by 57 nucleotides Pf1N1B4_1588 and Pf1N1B4_1589 are separated by 270 nucleotides Pf1N1B4_1589 and Pf1N1B4_1590 are separated by 23 nucleotides
Pf1N1B4_1587 - Urocanate hydratase (EC 4.2.1.49) (from data) , at 1,828,684 to 1,830,366
_1587
Pf1N1B4_1588 - hypothetical protein, at 1,830,424 to 1,830,546
_1588
Pf1N1B4_1589 - Conserved hypothetical protein (perhaps related to histidine degradation), at 1,830,817 to 1,831,386
_1589
Pf1N1B4_1590 - Histidine utilization repressor, at 1,831,410 to 1,832,171
_1590
Position (kb)
1830
1831 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1829.675 kb on + strand, within Pf1N1B4_1587 at 1829.675 kb on + strand, within Pf1N1B4_1587 at 1829.675 kb on + strand, within Pf1N1B4_1587 at 1829.675 kb on + strand, within Pf1N1B4_1587 at 1829.676 kb on - strand, within Pf1N1B4_1587 at 1829.676 kb on - strand, within Pf1N1B4_1587 at 1829.676 kb on - strand, within Pf1N1B4_1587 at 1829.676 kb on - strand, within Pf1N1B4_1587 at 1829.676 kb on - strand, within Pf1N1B4_1587 at 1829.676 kb on - strand, within Pf1N1B4_1587 at 1829.676 kb on - strand, within Pf1N1B4_1587 at 1829.676 kb on - strand, within Pf1N1B4_1587 at 1829.729 kb on + strand, within Pf1N1B4_1587 at 1829.730 kb on - strand, within Pf1N1B4_1587 at 1829.730 kb on - strand, within Pf1N1B4_1587 at 1829.877 kb on + strand, within Pf1N1B4_1587 at 1829.879 kb on + strand, within Pf1N1B4_1587 at 1829.879 kb on + strand, within Pf1N1B4_1587 at 1829.879 kb on + strand, within Pf1N1B4_1587 at 1829.879 kb on + strand, within Pf1N1B4_1587 at 1829.879 kb on + strand, within Pf1N1B4_1587 at 1829.879 kb on + strand, within Pf1N1B4_1587 at 1829.880 kb on - strand, within Pf1N1B4_1587 at 1829.880 kb on - strand, within Pf1N1B4_1587 at 1829.880 kb on - strand, within Pf1N1B4_1587 at 1829.880 kb on - strand, within Pf1N1B4_1587 at 1829.880 kb on - strand, within Pf1N1B4_1587 at 1829.912 kb on + strand, within Pf1N1B4_1587 at 1829.913 kb on - strand, within Pf1N1B4_1587 at 1829.913 kb on - strand, within Pf1N1B4_1587 at 1829.913 kb on - strand, within Pf1N1B4_1587 at 1829.913 kb on - strand, within Pf1N1B4_1587 at 1829.913 kb on - strand, within Pf1N1B4_1587 at 1829.945 kb on + strand, within Pf1N1B4_1587 at 1829.945 kb on + strand, within Pf1N1B4_1587 at 1829.946 kb on - strand, within Pf1N1B4_1587 at 1829.946 kb on - strand, within Pf1N1B4_1587 at 1829.975 kb on + strand, within Pf1N1B4_1587 at 1829.975 kb on + strand, within Pf1N1B4_1587 at 1829.976 kb on - strand, within Pf1N1B4_1587 at 1829.976 kb on - strand, within Pf1N1B4_1587 at 1830.167 kb on + strand, within Pf1N1B4_1587 at 1830.168 kb on - strand, within Pf1N1B4_1587 at 1830.168 kb on - strand, within Pf1N1B4_1587 at 1830.180 kb on + strand, within Pf1N1B4_1587 at 1830.180 kb on + strand, within Pf1N1B4_1587 at 1830.180 kb on + strand, within Pf1N1B4_1587 at 1830.180 kb on + strand, within Pf1N1B4_1587 at 1830.181 kb on - strand, within Pf1N1B4_1587 at 1830.181 kb on - strand, within Pf1N1B4_1587 at 1830.337 kb on - strand at 1830.341 kb on + strand at 1830.341 kb on + strand at 1830.341 kb on + strand at 1830.342 kb on - strand at 1830.342 kb on - strand at 1830.345 kb on + strand at 1830.345 kb on + strand at 1830.345 kb on + strand at 1830.345 kb on + strand at 1830.346 kb on - strand at 1830.346 kb on - strand at 1830.354 kb on + strand at 1830.354 kb on + strand at 1830.355 kb on - strand at 1830.485 kb on - strand, within Pf1N1B4_1588 at 1830.485 kb on - strand, within Pf1N1B4_1588 at 1830.490 kb on + strand, within Pf1N1B4_1588 at 1830.490 kb on + strand, within Pf1N1B4_1588 at 1830.491 kb on - strand, within Pf1N1B4_1588 at 1830.491 kb on - strand, within Pf1N1B4_1588 at 1830.491 kb on - strand, within Pf1N1B4_1588 at 1830.491 kb on - strand, within Pf1N1B4_1588 at 1830.499 kb on - strand, within Pf1N1B4_1588 at 1830.499 kb on - strand, within Pf1N1B4_1588 at 1830.504 kb on + strand, within Pf1N1B4_1588 at 1830.505 kb on - strand, within Pf1N1B4_1588 at 1830.508 kb on + strand, within Pf1N1B4_1588 at 1830.508 kb on + strand, within Pf1N1B4_1588 at 1830.509 kb on - strand, within Pf1N1B4_1588 at 1830.516 kb on + strand, within Pf1N1B4_1588 at 1830.517 kb on - strand, within Pf1N1B4_1588 at 1830.584 kb on + strand at 1830.584 kb on + strand at 1830.584 kb on + strand at 1830.585 kb on - strand at 1830.585 kb on - strand at 1830.585 kb on - strand at 1830.585 kb on - strand at 1830.648 kb on + strand at 1830.759 kb on + strand at 1830.759 kb on + strand at 1830.759 kb on + strand at 1830.759 kb on + strand at 1830.759 kb on + strand at 1830.759 kb on + strand at 1830.760 kb on - strand at 1830.865 kb on - strand at 1830.913 kb on + strand, within Pf1N1B4_1589 at 1831.049 kb on + strand at 1831.050 kb on - strand, within Pf1N1B4_1589 at 1831.050 kb on - strand, within Pf1N1B4_1589 at 1831.136 kb on - strand, within Pf1N1B4_1589 at 1831.345 kb on - strand at 1831.397 kb on + strand at 1831.397 kb on + strand at 1831.397 kb on + strand at 1831.397 kb on + strand at 1831.398 kb on - strand at 1831.398 kb on - strand at 1831.401 kb on + strand at 1831.401 kb on + strand at 1831.402 kb on - strand at 1831.402 kb on - strand at 1831.402 kb on - strand at 1831.411 kb on + strand at 1831.411 kb on + strand at 1831.411 kb on + strand at 1831.411 kb on + strand at 1831.411 kb on + strand at 1831.411 kb on + strand at 1831.412 kb on - strand at 1831.412 kb on - strand at 1831.412 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (C) remove 1,829,675 + Pf1N1B4_1587 0.59 -0.9 1,829,675 + Pf1N1B4_1587 0.59 -2.5 1,829,675 + Pf1N1B4_1587 0.59 -1.9 1,829,675 + Pf1N1B4_1587 0.59 +0.1 1,829,676 - Pf1N1B4_1587 0.59 -0.6 1,829,676 - Pf1N1B4_1587 0.59 -1.5 1,829,676 - Pf1N1B4_1587 0.59 -3.9 1,829,676 - Pf1N1B4_1587 0.59 -5.0 1,829,676 - Pf1N1B4_1587 0.59 -1.5 1,829,676 - Pf1N1B4_1587 0.59 -3.6 1,829,676 - Pf1N1B4_1587 0.59 -0.5 1,829,676 - Pf1N1B4_1587 0.59 -0.9 1,829,729 + Pf1N1B4_1587 0.62 -2.5 1,829,730 - Pf1N1B4_1587 0.62 -0.9 1,829,730 - Pf1N1B4_1587 0.62 -1.5 1,829,877 + Pf1N1B4_1587 0.71 -2.3 1,829,879 + Pf1N1B4_1587 0.71 +0.1 1,829,879 + Pf1N1B4_1587 0.71 -4.5 1,829,879 + Pf1N1B4_1587 0.71 -3.8 1,829,879 + Pf1N1B4_1587 0.71 -1.5 1,829,879 + Pf1N1B4_1587 0.71 -1.5 1,829,879 + Pf1N1B4_1587 0.71 -1.9 1,829,880 - Pf1N1B4_1587 0.71 -2.8 1,829,880 - Pf1N1B4_1587 0.71 -1.9 1,829,880 - Pf1N1B4_1587 0.71 -0.7 1,829,880 - Pf1N1B4_1587 0.71 +0.1 1,829,880 - Pf1N1B4_1587 0.71 -4.3 1,829,912 + Pf1N1B4_1587 0.73 -2.5 1,829,913 - Pf1N1B4_1587 0.73 -2.8 1,829,913 - Pf1N1B4_1587 0.73 -2.5 1,829,913 - Pf1N1B4_1587 0.73 -2.1 1,829,913 - Pf1N1B4_1587 0.73 -2.3 1,829,913 - Pf1N1B4_1587 0.73 -1.5 1,829,945 + Pf1N1B4_1587 0.75 -0.9 1,829,945 + Pf1N1B4_1587 0.75 +0.1 1,829,946 - Pf1N1B4_1587 0.75 -1.9 1,829,946 - Pf1N1B4_1587 0.75 -3.3 1,829,975 + Pf1N1B4_1587 0.77 -1.5 1,829,975 + Pf1N1B4_1587 0.77 -3.6 1,829,976 - Pf1N1B4_1587 0.77 -2.8 1,829,976 - Pf1N1B4_1587 0.77 -2.5 1,830,167 + Pf1N1B4_1587 0.88 -0.9 1,830,168 - Pf1N1B4_1587 0.88 -1.9 1,830,168 - Pf1N1B4_1587 0.88 -1.9 1,830,180 + Pf1N1B4_1587 0.89 -3.4 1,830,180 + Pf1N1B4_1587 0.89 +0.1 1,830,180 + Pf1N1B4_1587 0.89 -0.9 1,830,180 + Pf1N1B4_1587 0.89 -0.9 1,830,181 - Pf1N1B4_1587 0.89 -2.9 1,830,181 - Pf1N1B4_1587 0.89 +0.1 1,830,337 - -4.9 1,830,341 + -1.9 1,830,341 + -1.5 1,830,341 + -2.3 1,830,342 - -3.3 1,830,342 - -1.5 1,830,345 + -1.5 1,830,345 + -1.9 1,830,345 + -2.5 1,830,345 + -1.6 1,830,346 - +0.1 1,830,346 - +0.1 1,830,354 + -1.5 1,830,354 + -2.5 1,830,355 - +0.1 1,830,485 - Pf1N1B4_1588 0.50 -2.4 1,830,485 - Pf1N1B4_1588 0.50 -1.5 1,830,490 + Pf1N1B4_1588 0.54 -2.5 1,830,490 + Pf1N1B4_1588 0.54 -1.3 1,830,491 - Pf1N1B4_1588 0.54 -0.5 1,830,491 - Pf1N1B4_1588 0.54 -3.1 1,830,491 - Pf1N1B4_1588 0.54 -3.4 1,830,491 - Pf1N1B4_1588 0.54 -3.3 1,830,499 - Pf1N1B4_1588 0.61 -3.4 1,830,499 - Pf1N1B4_1588 0.61 -3.5 1,830,504 + Pf1N1B4_1588 0.65 -3.8 1,830,505 - Pf1N1B4_1588 0.66 -3.3 1,830,508 + Pf1N1B4_1588 0.68 +0.1 1,830,508 + Pf1N1B4_1588 0.68 -2.7 1,830,509 - Pf1N1B4_1588 0.69 -3.8 1,830,516 + Pf1N1B4_1588 0.75 -2.3 1,830,517 - Pf1N1B4_1588 0.76 -1.5 1,830,584 + -0.3 1,830,584 + -1.5 1,830,584 + +0.1 1,830,585 - -3.4 1,830,585 - -1.5 1,830,585 - -0.9 1,830,585 - +0.1 1,830,648 + +0.1 1,830,759 + +0.1 1,830,759 + -0.9 1,830,759 + -2.0 1,830,759 + -0.5 1,830,759 + -0.5 1,830,759 + +0.1 1,830,760 - +0.6 1,830,865 - -1.9 1,830,913 + Pf1N1B4_1589 0.17 +1.7 1,831,049 + -1.5 1,831,050 - Pf1N1B4_1589 0.41 -0.9 1,831,050 - Pf1N1B4_1589 0.41 -2.3 1,831,136 - Pf1N1B4_1589 0.56 -2.3 1,831,345 - -0.9 1,831,397 + -3.6 1,831,397 + -1.5 1,831,397 + +0.1 1,831,397 + -2.3 1,831,398 - +0.1 1,831,398 - -0.3 1,831,401 + -0.3 1,831,401 + -2.5 1,831,402 - -1.5 1,831,402 - +0.4 1,831,402 - -0.2 1,831,411 + -2.9 1,831,411 + -3.1 1,831,411 + -0.9 1,831,411 + +0.1 1,831,411 + -2.9 1,831,411 + -1.5 1,831,412 - +0.1 1,831,412 - +0.7 1,831,412 - +0.1
Or see this region's nucleotide sequence