Experiment: L-Histidine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1581 and Pf1N1B4_1582 are separated by 116 nucleotides Pf1N1B4_1582 and Pf1N1B4_1583 are separated by 158 nucleotides Pf1N1B4_1583 and Pf1N1B4_1584 overlap by 4 nucleotides
Pf1N1B4_1581 - Histidine ammonia-lyase (EC 4.3.1.3), at 1,820,960 to 1,822,492
_1581
Pf1N1B4_1582 - Histidine ammonia-lyase (EC 4.3.1.3) (from data) , at 1,822,609 to 1,824,132
_1582
Pf1N1B4_1583 - Histidine ABC transporter, ATP-binding protein (TC 3.A.1), at 1,824,291 to 1,825,121
_1583
Pf1N1B4_1584 - Histidine ABC transporter, permease protein (TC 3.A.1), at 1,825,118 to 1,825,969
_1584
Position (kb)
1822
1823
1824
1825 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1821.882 kb on + strand, within Pf1N1B4_1581 at 1821.882 kb on + strand, within Pf1N1B4_1581 at 1821.882 kb on + strand, within Pf1N1B4_1581 at 1821.883 kb on - strand, within Pf1N1B4_1581 at 1822.000 kb on - strand, within Pf1N1B4_1581 at 1822.009 kb on + strand, within Pf1N1B4_1581 at 1822.010 kb on - strand, within Pf1N1B4_1581 at 1822.435 kb on - strand at 1822.496 kb on + strand at 1822.521 kb on + strand at 1822.522 kb on - strand at 1822.522 kb on - strand at 1822.522 kb on - strand at 1822.597 kb on + strand at 1822.598 kb on - strand at 1822.598 kb on - strand at 1822.604 kb on - strand at 1822.604 kb on - strand at 1822.743 kb on + strand at 1822.805 kb on + strand, within Pf1N1B4_1582 at 1822.806 kb on - strand, within Pf1N1B4_1582 at 1822.806 kb on - strand, within Pf1N1B4_1582 at 1822.905 kb on + strand, within Pf1N1B4_1582 at 1822.905 kb on + strand, within Pf1N1B4_1582 at 1822.905 kb on + strand, within Pf1N1B4_1582 at 1822.905 kb on + strand, within Pf1N1B4_1582 at 1822.905 kb on + strand, within Pf1N1B4_1582 at 1822.906 kb on - strand, within Pf1N1B4_1582 at 1822.906 kb on - strand, within Pf1N1B4_1582 at 1822.906 kb on - strand, within Pf1N1B4_1582 at 1822.906 kb on - strand, within Pf1N1B4_1582 at 1822.910 kb on + strand, within Pf1N1B4_1582 at 1822.910 kb on + strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.973 kb on + strand, within Pf1N1B4_1582 at 1822.973 kb on + strand, within Pf1N1B4_1582 at 1822.974 kb on - strand, within Pf1N1B4_1582 at 1822.974 kb on - strand, within Pf1N1B4_1582 at 1822.974 kb on - strand, within Pf1N1B4_1582 at 1822.974 kb on - strand, within Pf1N1B4_1582 at 1822.974 kb on - strand, within Pf1N1B4_1582 at 1822.974 kb on - strand, within Pf1N1B4_1582 at 1822.974 kb on - strand, within Pf1N1B4_1582 at 1823.801 kb on + strand, within Pf1N1B4_1582 at 1823.801 kb on + strand, within Pf1N1B4_1582 at 1823.801 kb on + strand, within Pf1N1B4_1582 at 1823.801 kb on + strand, within Pf1N1B4_1582 at 1823.801 kb on + strand, within Pf1N1B4_1582 at 1823.801 kb on + strand, within Pf1N1B4_1582 at 1823.802 kb on - strand, within Pf1N1B4_1582 at 1823.802 kb on - strand, within Pf1N1B4_1582 at 1823.802 kb on - strand, within Pf1N1B4_1582 at 1823.802 kb on - strand, within Pf1N1B4_1582 at 1823.811 kb on + strand, within Pf1N1B4_1582 at 1823.964 kb on - strand, within Pf1N1B4_1582 at 1824.315 kb on + strand at 1824.315 kb on + strand at 1824.315 kb on + strand at 1824.316 kb on - strand at 1824.331 kb on + strand at 1824.331 kb on + strand at 1824.331 kb on + strand at 1824.331 kb on + strand at 1824.331 kb on + strand at 1824.332 kb on - strand at 1824.332 kb on - strand at 1824.332 kb on - strand at 1824.332 kb on - strand at 1824.332 kb on - strand at 1824.332 kb on - strand at 1824.629 kb on - strand, within Pf1N1B4_1583 at 1824.629 kb on - strand, within Pf1N1B4_1583 at 1824.629 kb on - strand, within Pf1N1B4_1583 at 1824.629 kb on - strand, within Pf1N1B4_1583 at 1824.629 kb on - strand, within Pf1N1B4_1583 at 1824.725 kb on - strand, within Pf1N1B4_1583 at 1824.725 kb on - strand, within Pf1N1B4_1583 at 1824.826 kb on + strand, within Pf1N1B4_1583 at 1824.826 kb on + strand, within Pf1N1B4_1583 at 1824.827 kb on - strand, within Pf1N1B4_1583 at 1824.827 kb on - strand, within Pf1N1B4_1583
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (C) remove 1,821,882 + Pf1N1B4_1581 0.60 +0.1 1,821,882 + Pf1N1B4_1581 0.60 -2.5 1,821,882 + Pf1N1B4_1581 0.60 -2.3 1,821,883 - Pf1N1B4_1581 0.60 -3.9 1,822,000 - Pf1N1B4_1581 0.68 -0.9 1,822,009 + Pf1N1B4_1581 0.68 -0.9 1,822,010 - Pf1N1B4_1581 0.68 -1.5 1,822,435 - -1.5 1,822,496 + -2.3 1,822,521 + +1.4 1,822,522 - -0.6 1,822,522 - -0.4 1,822,522 - +2.3 1,822,597 + -1.9 1,822,598 - +0.5 1,822,598 - -0.8 1,822,604 - -0.8 1,822,604 - -1.5 1,822,743 + -0.9 1,822,805 + Pf1N1B4_1582 0.13 -2.8 1,822,806 - Pf1N1B4_1582 0.13 +0.8 1,822,806 - Pf1N1B4_1582 0.13 +1.6 1,822,905 + Pf1N1B4_1582 0.19 -2.3 1,822,905 + Pf1N1B4_1582 0.19 -2.4 1,822,905 + Pf1N1B4_1582 0.19 -0.9 1,822,905 + Pf1N1B4_1582 0.19 -0.9 1,822,905 + Pf1N1B4_1582 0.19 -1.5 1,822,906 - Pf1N1B4_1582 0.19 -1.8 1,822,906 - Pf1N1B4_1582 0.19 +0.4 1,822,906 - Pf1N1B4_1582 0.19 -0.8 1,822,906 - Pf1N1B4_1582 0.19 -0.7 1,822,910 + Pf1N1B4_1582 0.20 -1.9 1,822,910 + Pf1N1B4_1582 0.20 -3.9 1,822,911 - Pf1N1B4_1582 0.20 +0.1 1,822,911 - Pf1N1B4_1582 0.20 -2.8 1,822,911 - Pf1N1B4_1582 0.20 -0.9 1,822,911 - Pf1N1B4_1582 0.20 -1.9 1,822,911 - Pf1N1B4_1582 0.20 -0.9 1,822,911 - Pf1N1B4_1582 0.20 -0.3 1,822,911 - Pf1N1B4_1582 0.20 -1.3 1,822,911 - Pf1N1B4_1582 0.20 -0.7 1,822,911 - Pf1N1B4_1582 0.20 -1.6 1,822,911 - Pf1N1B4_1582 0.20 +0.6 1,822,911 - Pf1N1B4_1582 0.20 +0.1 1,822,911 - Pf1N1B4_1582 0.20 -1.5 1,822,911 - Pf1N1B4_1582 0.20 -0.9 1,822,973 + Pf1N1B4_1582 0.24 -1.5 1,822,973 + Pf1N1B4_1582 0.24 -2.5 1,822,974 - Pf1N1B4_1582 0.24 +0.1 1,822,974 - Pf1N1B4_1582 0.24 +0.6 1,822,974 - Pf1N1B4_1582 0.24 -1.6 1,822,974 - Pf1N1B4_1582 0.24 -1.5 1,822,974 - Pf1N1B4_1582 0.24 +0.1 1,822,974 - Pf1N1B4_1582 0.24 -0.9 1,822,974 - Pf1N1B4_1582 0.24 -0.9 1,823,801 + Pf1N1B4_1582 0.78 -2.3 1,823,801 + Pf1N1B4_1582 0.78 -1.5 1,823,801 + Pf1N1B4_1582 0.78 -2.5 1,823,801 + Pf1N1B4_1582 0.78 -1.5 1,823,801 + Pf1N1B4_1582 0.78 -2.8 1,823,801 + Pf1N1B4_1582 0.78 -1.3 1,823,802 - Pf1N1B4_1582 0.78 -3.3 1,823,802 - Pf1N1B4_1582 0.78 -1.5 1,823,802 - Pf1N1B4_1582 0.78 +0.1 1,823,802 - Pf1N1B4_1582 0.78 -1.9 1,823,811 + Pf1N1B4_1582 0.79 -1.5 1,823,964 - Pf1N1B4_1582 0.89 -2.8 1,824,315 + -2.9 1,824,315 + -1.9 1,824,315 + -2.8 1,824,316 - +0.1 1,824,331 + -1.5 1,824,331 + -1.9 1,824,331 + -2.3 1,824,331 + -1.5 1,824,331 + -2.3 1,824,332 - -1.9 1,824,332 - -2.3 1,824,332 - -2.1 1,824,332 - -0.9 1,824,332 - -1.5 1,824,332 - +0.1 1,824,629 - Pf1N1B4_1583 0.41 -0.9 1,824,629 - Pf1N1B4_1583 0.41 -2.3 1,824,629 - Pf1N1B4_1583 0.41 -2.5 1,824,629 - Pf1N1B4_1583 0.41 -0.9 1,824,629 - Pf1N1B4_1583 0.41 +0.1 1,824,725 - Pf1N1B4_1583 0.52 -3.1 1,824,725 - Pf1N1B4_1583 0.52 -1.9 1,824,826 + Pf1N1B4_1583 0.64 -2.5 1,824,826 + Pf1N1B4_1583 0.64 -1.3 1,824,827 - Pf1N1B4_1583 0.65 -1.8 1,824,827 - Pf1N1B4_1583 0.65 -2.3
Or see this region's nucleotide sequence