Experiment: L-Histidine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1580 and Pf1N1B4_1581 are separated by 151 nucleotides Pf1N1B4_1581 and Pf1N1B4_1582 are separated by 116 nucleotides
Pf1N1B4_1580 - Histidine transport protein (permease), at 1,819,399 to 1,820,808
_1580
Pf1N1B4_1581 - Histidine ammonia-lyase (EC 4.3.1.3), at 1,820,960 to 1,822,492
_1581
Pf1N1B4_1582 - Histidine ammonia-lyase (EC 4.3.1.3) (from data) , at 1,822,609 to 1,824,132
_1582
Position (kb)
1820
1821
1822
1823 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1820.060 kb on + strand, within Pf1N1B4_1580 at 1820.060 kb on + strand, within Pf1N1B4_1580 at 1820.060 kb on + strand, within Pf1N1B4_1580 at 1820.061 kb on - strand, within Pf1N1B4_1580 at 1820.061 kb on - strand, within Pf1N1B4_1580 at 1820.061 kb on - strand, within Pf1N1B4_1580 at 1820.061 kb on - strand, within Pf1N1B4_1580 at 1820.061 kb on - strand, within Pf1N1B4_1580 at 1820.073 kb on + strand, within Pf1N1B4_1580 at 1820.073 kb on + strand, within Pf1N1B4_1580 at 1820.073 kb on + strand, within Pf1N1B4_1580 at 1820.073 kb on + strand, within Pf1N1B4_1580 at 1820.073 kb on + strand, within Pf1N1B4_1580 at 1820.146 kb on + strand, within Pf1N1B4_1580 at 1820.386 kb on - strand, within Pf1N1B4_1580 at 1820.386 kb on - strand, within Pf1N1B4_1580 at 1820.468 kb on + strand, within Pf1N1B4_1580 at 1820.468 kb on + strand, within Pf1N1B4_1580 at 1820.468 kb on + strand, within Pf1N1B4_1580 at 1820.468 kb on + strand, within Pf1N1B4_1580 at 1820.468 kb on + strand, within Pf1N1B4_1580 at 1820.468 kb on + strand, within Pf1N1B4_1580 at 1820.468 kb on + strand, within Pf1N1B4_1580 at 1820.468 kb on + strand, within Pf1N1B4_1580 at 1820.468 kb on + strand, within Pf1N1B4_1580 at 1820.468 kb on + strand, within Pf1N1B4_1580 at 1820.468 kb on + strand, within Pf1N1B4_1580 at 1820.468 kb on + strand, within Pf1N1B4_1580 at 1820.468 kb on + strand, within Pf1N1B4_1580 at 1820.468 kb on + strand, within Pf1N1B4_1580 at 1820.468 kb on + strand, within Pf1N1B4_1580 at 1820.469 kb on - strand, within Pf1N1B4_1580 at 1820.469 kb on - strand, within Pf1N1B4_1580 at 1820.469 kb on - strand at 1820.469 kb on - strand, within Pf1N1B4_1580 at 1820.469 kb on - strand, within Pf1N1B4_1580 at 1820.523 kb on - strand, within Pf1N1B4_1580 at 1820.557 kb on + strand, within Pf1N1B4_1580 at 1820.557 kb on + strand, within Pf1N1B4_1580 at 1820.558 kb on - strand, within Pf1N1B4_1580 at 1820.558 kb on - strand, within Pf1N1B4_1580 at 1820.558 kb on - strand, within Pf1N1B4_1580 at 1820.561 kb on + strand, within Pf1N1B4_1580 at 1820.561 kb on + strand, within Pf1N1B4_1580 at 1820.561 kb on + strand, within Pf1N1B4_1580 at 1820.561 kb on + strand, within Pf1N1B4_1580 at 1820.561 kb on + strand, within Pf1N1B4_1580 at 1820.561 kb on + strand, within Pf1N1B4_1580 at 1820.562 kb on - strand, within Pf1N1B4_1580 at 1820.562 kb on - strand, within Pf1N1B4_1580 at 1820.562 kb on - strand, within Pf1N1B4_1580 at 1820.562 kb on - strand, within Pf1N1B4_1580 at 1820.562 kb on - strand, within Pf1N1B4_1580 at 1820.562 kb on - strand, within Pf1N1B4_1580 at 1820.663 kb on + strand, within Pf1N1B4_1580 at 1820.663 kb on + strand, within Pf1N1B4_1580 at 1820.663 kb on + strand, within Pf1N1B4_1580 at 1820.663 kb on + strand, within Pf1N1B4_1580 at 1820.664 kb on - strand, within Pf1N1B4_1580 at 1820.714 kb on + strand at 1820.714 kb on + strand at 1820.715 kb on - strand at 1820.715 kb on - strand at 1820.715 kb on - strand at 1820.760 kb on + strand at 1820.760 kb on + strand at 1820.760 kb on + strand at 1820.760 kb on + strand at 1820.761 kb on - strand at 1820.819 kb on + strand at 1820.819 kb on + strand at 1820.824 kb on - strand at 1820.824 kb on - strand at 1821.066 kb on + strand at 1821.066 kb on + strand at 1821.066 kb on + strand at 1821.067 kb on - strand at 1821.067 kb on - strand at 1821.067 kb on - strand at 1821.067 kb on - strand at 1821.508 kb on + strand, within Pf1N1B4_1581 at 1821.509 kb on - strand, within Pf1N1B4_1581 at 1821.509 kb on - strand, within Pf1N1B4_1581 at 1821.882 kb on + strand, within Pf1N1B4_1581 at 1821.882 kb on + strand, within Pf1N1B4_1581 at 1821.882 kb on + strand, within Pf1N1B4_1581 at 1821.883 kb on - strand, within Pf1N1B4_1581 at 1822.000 kb on - strand, within Pf1N1B4_1581 at 1822.009 kb on + strand, within Pf1N1B4_1581 at 1822.010 kb on - strand, within Pf1N1B4_1581 at 1822.435 kb on - strand at 1822.496 kb on + strand at 1822.521 kb on + strand at 1822.522 kb on - strand at 1822.522 kb on - strand at 1822.522 kb on - strand at 1822.597 kb on + strand at 1822.598 kb on - strand at 1822.598 kb on - strand at 1822.604 kb on - strand at 1822.604 kb on - strand at 1822.743 kb on + strand at 1822.805 kb on + strand, within Pf1N1B4_1582 at 1822.806 kb on - strand, within Pf1N1B4_1582 at 1822.806 kb on - strand, within Pf1N1B4_1582 at 1822.905 kb on + strand, within Pf1N1B4_1582 at 1822.905 kb on + strand, within Pf1N1B4_1582 at 1822.905 kb on + strand, within Pf1N1B4_1582 at 1822.905 kb on + strand, within Pf1N1B4_1582 at 1822.905 kb on + strand, within Pf1N1B4_1582 at 1822.906 kb on - strand, within Pf1N1B4_1582 at 1822.906 kb on - strand, within Pf1N1B4_1582 at 1822.906 kb on - strand, within Pf1N1B4_1582 at 1822.906 kb on - strand, within Pf1N1B4_1582 at 1822.910 kb on + strand, within Pf1N1B4_1582 at 1822.910 kb on + strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.911 kb on - strand, within Pf1N1B4_1582 at 1822.973 kb on + strand, within Pf1N1B4_1582 at 1822.973 kb on + strand, within Pf1N1B4_1582 at 1822.974 kb on - strand, within Pf1N1B4_1582 at 1822.974 kb on - strand, within Pf1N1B4_1582 at 1822.974 kb on - strand, within Pf1N1B4_1582 at 1822.974 kb on - strand, within Pf1N1B4_1582 at 1822.974 kb on - strand, within Pf1N1B4_1582 at 1822.974 kb on - strand, within Pf1N1B4_1582 at 1822.974 kb on - strand, within Pf1N1B4_1582
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (C) remove 1,820,060 + Pf1N1B4_1580 0.47 -2.5 1,820,060 + Pf1N1B4_1580 0.47 -0.9 1,820,060 + Pf1N1B4_1580 0.47 -2.8 1,820,061 - Pf1N1B4_1580 0.47 -2.8 1,820,061 - Pf1N1B4_1580 0.47 -2.3 1,820,061 - Pf1N1B4_1580 0.47 -0.4 1,820,061 - Pf1N1B4_1580 0.47 -1.9 1,820,061 - Pf1N1B4_1580 0.47 -0.9 1,820,073 + Pf1N1B4_1580 0.48 -1.5 1,820,073 + Pf1N1B4_1580 0.48 -3.8 1,820,073 + Pf1N1B4_1580 0.48 -2.5 1,820,073 + Pf1N1B4_1580 0.48 -2.5 1,820,073 + Pf1N1B4_1580 0.48 -1.5 1,820,146 + Pf1N1B4_1580 0.53 -1.5 1,820,386 - Pf1N1B4_1580 0.70 -2.8 1,820,386 - Pf1N1B4_1580 0.70 -0.9 1,820,468 + Pf1N1B4_1580 0.76 -2.5 1,820,468 + Pf1N1B4_1580 0.76 -1.5 1,820,468 + Pf1N1B4_1580 0.76 -2.3 1,820,468 + Pf1N1B4_1580 0.76 +0.1 1,820,468 + Pf1N1B4_1580 0.76 -1.3 1,820,468 + Pf1N1B4_1580 0.76 -0.9 1,820,468 + Pf1N1B4_1580 0.76 -2.8 1,820,468 + Pf1N1B4_1580 0.76 -2.3 1,820,468 + Pf1N1B4_1580 0.76 +0.1 1,820,468 + Pf1N1B4_1580 0.76 -1.9 1,820,468 + Pf1N1B4_1580 0.76 -2.5 1,820,468 + Pf1N1B4_1580 0.76 -2.8 1,820,468 + Pf1N1B4_1580 0.76 -2.5 1,820,468 + Pf1N1B4_1580 0.76 -2.3 1,820,468 + Pf1N1B4_1580 0.76 -0.4 1,820,469 - Pf1N1B4_1580 0.76 -2.5 1,820,469 - Pf1N1B4_1580 0.76 -2.8 1,820,469 - +0.1 1,820,469 - Pf1N1B4_1580 0.76 -1.5 1,820,469 - Pf1N1B4_1580 0.76 +0.1 1,820,523 - Pf1N1B4_1580 0.80 -1.5 1,820,557 + Pf1N1B4_1580 0.82 -2.3 1,820,557 + Pf1N1B4_1580 0.82 -1.9 1,820,558 - Pf1N1B4_1580 0.82 -1.5 1,820,558 - Pf1N1B4_1580 0.82 -1.5 1,820,558 - Pf1N1B4_1580 0.82 -3.9 1,820,561 + Pf1N1B4_1580 0.82 -1.5 1,820,561 + Pf1N1B4_1580 0.82 -2.8 1,820,561 + Pf1N1B4_1580 0.82 -2.9 1,820,561 + Pf1N1B4_1580 0.82 -1.3 1,820,561 + Pf1N1B4_1580 0.82 -2.5 1,820,561 + Pf1N1B4_1580 0.82 -1.4 1,820,562 - Pf1N1B4_1580 0.82 -1.5 1,820,562 - Pf1N1B4_1580 0.82 -2.8 1,820,562 - Pf1N1B4_1580 0.82 -3.1 1,820,562 - Pf1N1B4_1580 0.82 -0.9 1,820,562 - Pf1N1B4_1580 0.82 -2.9 1,820,562 - Pf1N1B4_1580 0.82 -2.8 1,820,663 + Pf1N1B4_1580 0.90 -4.9 1,820,663 + Pf1N1B4_1580 0.90 -1.7 1,820,663 + Pf1N1B4_1580 0.90 -1.9 1,820,663 + Pf1N1B4_1580 0.90 -0.9 1,820,664 - Pf1N1B4_1580 0.90 -3.1 1,820,714 + -0.5 1,820,714 + -0.9 1,820,715 - -2.3 1,820,715 - -0.5 1,820,715 - +2.1 1,820,760 + -2.3 1,820,760 + -1.5 1,820,760 + -0.9 1,820,760 + -1.9 1,820,761 - -3.1 1,820,819 + -1.5 1,820,819 + -2.5 1,820,824 - +1.1 1,820,824 - +1.6 1,821,066 + +0.1 1,821,066 + -1.5 1,821,066 + -1.5 1,821,067 - -1.5 1,821,067 - -1.9 1,821,067 - -1.5 1,821,067 - -2.8 1,821,508 + Pf1N1B4_1581 0.36 -0.9 1,821,509 - Pf1N1B4_1581 0.36 -3.1 1,821,509 - Pf1N1B4_1581 0.36 -3.6 1,821,882 + Pf1N1B4_1581 0.60 +0.1 1,821,882 + Pf1N1B4_1581 0.60 -2.5 1,821,882 + Pf1N1B4_1581 0.60 -2.3 1,821,883 - Pf1N1B4_1581 0.60 -3.9 1,822,000 - Pf1N1B4_1581 0.68 -0.9 1,822,009 + Pf1N1B4_1581 0.68 -0.9 1,822,010 - Pf1N1B4_1581 0.68 -1.5 1,822,435 - -1.5 1,822,496 + -2.3 1,822,521 + +1.4 1,822,522 - -0.6 1,822,522 - -0.4 1,822,522 - +2.3 1,822,597 + -1.9 1,822,598 - +0.5 1,822,598 - -0.8 1,822,604 - -0.8 1,822,604 - -1.5 1,822,743 + -0.9 1,822,805 + Pf1N1B4_1582 0.13 -2.8 1,822,806 - Pf1N1B4_1582 0.13 +0.8 1,822,806 - Pf1N1B4_1582 0.13 +1.6 1,822,905 + Pf1N1B4_1582 0.19 -2.3 1,822,905 + Pf1N1B4_1582 0.19 -2.4 1,822,905 + Pf1N1B4_1582 0.19 -0.9 1,822,905 + Pf1N1B4_1582 0.19 -0.9 1,822,905 + Pf1N1B4_1582 0.19 -1.5 1,822,906 - Pf1N1B4_1582 0.19 -1.8 1,822,906 - Pf1N1B4_1582 0.19 +0.4 1,822,906 - Pf1N1B4_1582 0.19 -0.8 1,822,906 - Pf1N1B4_1582 0.19 -0.7 1,822,910 + Pf1N1B4_1582 0.20 -1.9 1,822,910 + Pf1N1B4_1582 0.20 -3.9 1,822,911 - Pf1N1B4_1582 0.20 +0.1 1,822,911 - Pf1N1B4_1582 0.20 -2.8 1,822,911 - Pf1N1B4_1582 0.20 -0.9 1,822,911 - Pf1N1B4_1582 0.20 -1.9 1,822,911 - Pf1N1B4_1582 0.20 -0.9 1,822,911 - Pf1N1B4_1582 0.20 -0.3 1,822,911 - Pf1N1B4_1582 0.20 -1.3 1,822,911 - Pf1N1B4_1582 0.20 -0.7 1,822,911 - Pf1N1B4_1582 0.20 -1.6 1,822,911 - Pf1N1B4_1582 0.20 +0.6 1,822,911 - Pf1N1B4_1582 0.20 +0.1 1,822,911 - Pf1N1B4_1582 0.20 -1.5 1,822,911 - Pf1N1B4_1582 0.20 -0.9 1,822,973 + Pf1N1B4_1582 0.24 -1.5 1,822,973 + Pf1N1B4_1582 0.24 -2.5 1,822,974 - Pf1N1B4_1582 0.24 +0.1 1,822,974 - Pf1N1B4_1582 0.24 +0.6 1,822,974 - Pf1N1B4_1582 0.24 -1.6 1,822,974 - Pf1N1B4_1582 0.24 -1.5 1,822,974 - Pf1N1B4_1582 0.24 +0.1 1,822,974 - Pf1N1B4_1582 0.24 -0.9 1,822,974 - Pf1N1B4_1582 0.24 -0.9
Or see this region's nucleotide sequence