Experiment: L-Histidine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1577 and Pf1N1B4_1578 are separated by 181 nucleotides Pf1N1B4_1578 and Pf1N1B4_1579 are separated by 10 nucleotides
Pf1N1B4_1577 - L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1), at 1,816,227 to 1,817,183
_1577
Pf1N1B4_1578 - N-formylglutamate deformylase (EC 3.5.1.68) (from data) , at 1,817,365 to 1,818,165
_1578
Pf1N1B4_1579 - Imidazolonepropionase (EC 3.5.2.7) (from data) , at 1,818,176 to 1,819,381
_1579
Position (kb)
1817
1818
1819 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1816.453 kb on + strand, within Pf1N1B4_1577 at 1816.454 kb on - strand, within Pf1N1B4_1577 at 1816.528 kb on + strand, within Pf1N1B4_1577 at 1816.528 kb on + strand, within Pf1N1B4_1577 at 1816.528 kb on + strand at 1816.528 kb on + strand, within Pf1N1B4_1577 at 1816.528 kb on + strand, within Pf1N1B4_1577 at 1816.528 kb on + strand, within Pf1N1B4_1577 at 1816.529 kb on - strand, within Pf1N1B4_1577 at 1816.529 kb on - strand, within Pf1N1B4_1577 at 1816.529 kb on - strand, within Pf1N1B4_1577 at 1816.529 kb on - strand, within Pf1N1B4_1577 at 1816.529 kb on - strand, within Pf1N1B4_1577 at 1816.529 kb on - strand, within Pf1N1B4_1577 at 1816.529 kb on - strand, within Pf1N1B4_1577 at 1816.804 kb on + strand, within Pf1N1B4_1577 at 1816.804 kb on + strand, within Pf1N1B4_1577 at 1816.804 kb on + strand, within Pf1N1B4_1577 at 1816.805 kb on - strand, within Pf1N1B4_1577 at 1816.805 kb on - strand, within Pf1N1B4_1577 at 1816.805 kb on - strand, within Pf1N1B4_1577 at 1816.805 kb on - strand, within Pf1N1B4_1577 at 1816.805 kb on - strand, within Pf1N1B4_1577 at 1816.805 kb on - strand, within Pf1N1B4_1577 at 1816.805 kb on - strand, within Pf1N1B4_1577 at 1816.898 kb on + strand, within Pf1N1B4_1577 at 1816.931 kb on + strand, within Pf1N1B4_1577 at 1817.019 kb on + strand, within Pf1N1B4_1577 at 1817.019 kb on + strand, within Pf1N1B4_1577 at 1817.019 kb on + strand, within Pf1N1B4_1577 at 1817.019 kb on + strand, within Pf1N1B4_1577 at 1817.019 kb on + strand, within Pf1N1B4_1577 at 1817.020 kb on - strand, within Pf1N1B4_1577 at 1817.020 kb on - strand, within Pf1N1B4_1577 at 1817.020 kb on - strand, within Pf1N1B4_1577 at 1817.020 kb on - strand, within Pf1N1B4_1577 at 1817.020 kb on - strand, within Pf1N1B4_1577 at 1817.020 kb on - strand, within Pf1N1B4_1577 at 1817.020 kb on - strand, within Pf1N1B4_1577 at 1817.076 kb on + strand, within Pf1N1B4_1577 at 1817.184 kb on - strand at 1817.208 kb on + strand at 1817.209 kb on - strand at 1817.251 kb on + strand at 1817.251 kb on + strand at 1817.251 kb on + strand at 1817.252 kb on - strand at 1817.252 kb on - strand at 1817.282 kb on + strand at 1817.283 kb on - strand at 1817.283 kb on - strand at 1817.324 kb on - strand at 1817.480 kb on + strand, within Pf1N1B4_1578 at 1817.480 kb on + strand, within Pf1N1B4_1578 at 1817.480 kb on + strand, within Pf1N1B4_1578 at 1817.480 kb on + strand, within Pf1N1B4_1578 at 1817.480 kb on + strand, within Pf1N1B4_1578 at 1817.480 kb on + strand, within Pf1N1B4_1578 at 1817.480 kb on + strand, within Pf1N1B4_1578 at 1817.480 kb on + strand, within Pf1N1B4_1578 at 1817.481 kb on - strand, within Pf1N1B4_1578 at 1817.481 kb on - strand, within Pf1N1B4_1578 at 1817.481 kb on - strand, within Pf1N1B4_1578 at 1817.481 kb on - strand, within Pf1N1B4_1578 at 1817.481 kb on - strand, within Pf1N1B4_1578 at 1817.481 kb on - strand, within Pf1N1B4_1578 at 1817.494 kb on + strand, within Pf1N1B4_1578 at 1817.589 kb on - strand, within Pf1N1B4_1578 at 1817.589 kb on - strand, within Pf1N1B4_1578 at 1817.795 kb on + strand, within Pf1N1B4_1578 at 1817.819 kb on + strand, within Pf1N1B4_1578 at 1817.895 kb on - strand, within Pf1N1B4_1578 at 1817.896 kb on + strand, within Pf1N1B4_1578 at 1817.896 kb on + strand, within Pf1N1B4_1578 at 1817.896 kb on + strand, within Pf1N1B4_1578 at 1817.896 kb on + strand, within Pf1N1B4_1578 at 1817.897 kb on - strand, within Pf1N1B4_1578 at 1817.897 kb on - strand, within Pf1N1B4_1578 at 1817.897 kb on - strand, within Pf1N1B4_1578 at 1817.918 kb on + strand, within Pf1N1B4_1578 at 1817.918 kb on + strand, within Pf1N1B4_1578 at 1817.918 kb on + strand, within Pf1N1B4_1578 at 1817.918 kb on + strand at 1817.918 kb on + strand, within Pf1N1B4_1578 at 1817.918 kb on + strand, within Pf1N1B4_1578 at 1817.918 kb on + strand, within Pf1N1B4_1578 at 1817.919 kb on - strand, within Pf1N1B4_1578 at 1817.919 kb on - strand, within Pf1N1B4_1578 at 1817.919 kb on - strand, within Pf1N1B4_1578 at 1817.919 kb on - strand at 1817.919 kb on - strand, within Pf1N1B4_1578 at 1817.919 kb on - strand, within Pf1N1B4_1578 at 1817.919 kb on - strand, within Pf1N1B4_1578 at 1817.919 kb on - strand, within Pf1N1B4_1578 at 1817.919 kb on - strand, within Pf1N1B4_1578 at 1817.920 kb on + strand, within Pf1N1B4_1578 at 1817.921 kb on - strand, within Pf1N1B4_1578 at 1817.921 kb on - strand, within Pf1N1B4_1578 at 1817.921 kb on - strand, within Pf1N1B4_1578 at 1817.969 kb on + strand, within Pf1N1B4_1578 at 1818.159 kb on + strand at 1818.238 kb on - strand at 1818.525 kb on + strand, within Pf1N1B4_1579 at 1818.526 kb on - strand, within Pf1N1B4_1579 at 1818.831 kb on + strand, within Pf1N1B4_1579 at 1818.831 kb on + strand, within Pf1N1B4_1579 at 1818.831 kb on + strand, within Pf1N1B4_1579 at 1818.832 kb on - strand, within Pf1N1B4_1579 at 1818.832 kb on - strand, within Pf1N1B4_1579 at 1818.832 kb on - strand, within Pf1N1B4_1579 at 1818.852 kb on + strand, within Pf1N1B4_1579 at 1818.853 kb on - strand, within Pf1N1B4_1579 at 1819.120 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (C) remove 1,816,453 + Pf1N1B4_1577 0.24 -1.5 1,816,454 - Pf1N1B4_1577 0.24 +0.1 1,816,528 + Pf1N1B4_1577 0.31 -0.3 1,816,528 + Pf1N1B4_1577 0.31 -0.8 1,816,528 + -0.9 1,816,528 + Pf1N1B4_1577 0.31 -0.4 1,816,528 + Pf1N1B4_1577 0.31 -0.5 1,816,528 + Pf1N1B4_1577 0.31 +1.1 1,816,529 - Pf1N1B4_1577 0.32 -1.5 1,816,529 - Pf1N1B4_1577 0.32 +0.1 1,816,529 - Pf1N1B4_1577 0.32 +1.3 1,816,529 - Pf1N1B4_1577 0.32 -0.7 1,816,529 - Pf1N1B4_1577 0.32 -0.2 1,816,529 - Pf1N1B4_1577 0.32 -1.9 1,816,529 - Pf1N1B4_1577 0.32 -0.5 1,816,804 + Pf1N1B4_1577 0.60 +0.9 1,816,804 + Pf1N1B4_1577 0.60 -1.1 1,816,804 + Pf1N1B4_1577 0.60 -1.7 1,816,805 - Pf1N1B4_1577 0.60 +1.6 1,816,805 - Pf1N1B4_1577 0.60 +1.1 1,816,805 - Pf1N1B4_1577 0.60 -2.3 1,816,805 - Pf1N1B4_1577 0.60 +1.5 1,816,805 - Pf1N1B4_1577 0.60 +0.1 1,816,805 - Pf1N1B4_1577 0.60 -1.2 1,816,805 - Pf1N1B4_1577 0.60 -0.5 1,816,898 + Pf1N1B4_1577 0.70 +0.6 1,816,931 + Pf1N1B4_1577 0.74 +0.1 1,817,019 + Pf1N1B4_1577 0.83 +0.1 1,817,019 + Pf1N1B4_1577 0.83 +1.9 1,817,019 + Pf1N1B4_1577 0.83 +0.7 1,817,019 + Pf1N1B4_1577 0.83 -0.5 1,817,019 + Pf1N1B4_1577 0.83 +1.6 1,817,020 - Pf1N1B4_1577 0.83 -1.5 1,817,020 - Pf1N1B4_1577 0.83 -0.9 1,817,020 - Pf1N1B4_1577 0.83 +0.1 1,817,020 - Pf1N1B4_1577 0.83 +1.3 1,817,020 - Pf1N1B4_1577 0.83 +0.2 1,817,020 - Pf1N1B4_1577 0.83 -0.4 1,817,020 - Pf1N1B4_1577 0.83 +0.5 1,817,076 + Pf1N1B4_1577 0.89 +1.1 1,817,184 - -0.6 1,817,208 + -0.5 1,817,209 - -1.5 1,817,251 + +1.2 1,817,251 + +0.6 1,817,251 + -0.7 1,817,252 - -1.9 1,817,252 - +0.1 1,817,282 + +0.8 1,817,283 - +1.6 1,817,283 - -2.4 1,817,324 - +0.1 1,817,480 + Pf1N1B4_1578 0.14 -2.9 1,817,480 + Pf1N1B4_1578 0.14 -2.3 1,817,480 + Pf1N1B4_1578 0.14 -1.5 1,817,480 + Pf1N1B4_1578 0.14 +0.1 1,817,480 + Pf1N1B4_1578 0.14 +0.1 1,817,480 + Pf1N1B4_1578 0.14 -1.9 1,817,480 + Pf1N1B4_1578 0.14 -1.5 1,817,480 + Pf1N1B4_1578 0.14 -3.4 1,817,481 - Pf1N1B4_1578 0.14 -0.9 1,817,481 - Pf1N1B4_1578 0.14 -1.5 1,817,481 - Pf1N1B4_1578 0.14 -0.7 1,817,481 - Pf1N1B4_1578 0.14 -2.2 1,817,481 - Pf1N1B4_1578 0.14 -2.5 1,817,481 - Pf1N1B4_1578 0.14 -1.9 1,817,494 + Pf1N1B4_1578 0.16 -2.3 1,817,589 - Pf1N1B4_1578 0.28 -0.9 1,817,589 - Pf1N1B4_1578 0.28 -0.9 1,817,795 + Pf1N1B4_1578 0.54 -2.3 1,817,819 + Pf1N1B4_1578 0.57 -0.9 1,817,895 - Pf1N1B4_1578 0.66 -3.1 1,817,896 + Pf1N1B4_1578 0.66 -2.3 1,817,896 + Pf1N1B4_1578 0.66 -3.4 1,817,896 + Pf1N1B4_1578 0.66 -1.9 1,817,896 + Pf1N1B4_1578 0.66 -3.6 1,817,897 - Pf1N1B4_1578 0.66 -0.9 1,817,897 - Pf1N1B4_1578 0.66 -2.5 1,817,897 - Pf1N1B4_1578 0.66 -1.5 1,817,918 + Pf1N1B4_1578 0.69 -3.5 1,817,918 + Pf1N1B4_1578 0.69 -3.4 1,817,918 + Pf1N1B4_1578 0.69 -2.3 1,817,918 + +0.1 1,817,918 + Pf1N1B4_1578 0.69 -1.4 1,817,918 + Pf1N1B4_1578 0.69 +1.1 1,817,918 + Pf1N1B4_1578 0.69 -0.9 1,817,919 - Pf1N1B4_1578 0.69 -2.3 1,817,919 - Pf1N1B4_1578 0.69 -1.5 1,817,919 - Pf1N1B4_1578 0.69 -0.9 1,817,919 - +0.1 1,817,919 - Pf1N1B4_1578 0.69 +0.1 1,817,919 - Pf1N1B4_1578 0.69 -2.3 1,817,919 - Pf1N1B4_1578 0.69 -4.2 1,817,919 - Pf1N1B4_1578 0.69 -0.9 1,817,919 - Pf1N1B4_1578 0.69 -2.3 1,817,920 + Pf1N1B4_1578 0.69 -3.4 1,817,921 - Pf1N1B4_1578 0.69 +0.1 1,817,921 - Pf1N1B4_1578 0.69 +0.1 1,817,921 - Pf1N1B4_1578 0.69 -1.9 1,817,969 + Pf1N1B4_1578 0.75 -3.5 1,818,159 + -2.1 1,818,238 - -1.5 1,818,525 + Pf1N1B4_1579 0.29 -3.6 1,818,526 - Pf1N1B4_1579 0.29 -1.9 1,818,831 + Pf1N1B4_1579 0.54 -3.9 1,818,831 + Pf1N1B4_1579 0.54 -2.8 1,818,831 + Pf1N1B4_1579 0.54 -2.8 1,818,832 - Pf1N1B4_1579 0.54 -0.9 1,818,832 - Pf1N1B4_1579 0.54 -1.5 1,818,832 - Pf1N1B4_1579 0.54 -2.8 1,818,852 + Pf1N1B4_1579 0.56 -3.4 1,818,853 - Pf1N1B4_1579 0.56 -0.9 1,819,120 - +0.1
Or see this region's nucleotide sequence