Experiment: LB, pH10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_723 and Ac3H11_724 are separated by 79 nucleotides Ac3H11_724 and Ac3H11_725 are separated by 133 nucleotides Ac3H11_725 and Ac3H11_726 overlap by 4 nucleotides Ac3H11_726 and Ac3H11_727 overlap by 1 nucleotides
Ac3H11_723 - Cell division protein FtsN, at 13,445 to 14,107
_723
Ac3H11_724 - Periplasmic thiol:disulfide interchange protein DsbA, at 14,187 to 14,834
_724
Ac3H11_725 - LptA, protein essential for LPS transport across the periplasm, at 14,968 to 15,624
_725
Ac3H11_726 - Lipopolysaccharide ABC transporter, ATP-binding protein LptB, at 15,621 to 16,403
_726
Ac3H11_727 - RNA polymerase sigma-54 factor RpoN, at 16,403 to 17,986
_727
Position (kb) 14
15
16 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 14.171 kb on + strand at 14.171 kb on + strand at 14.171 kb on + strand at 14.171 kb on + strand at 14.833 kb on - strand at 14.833 kb on - strand at 14.833 kb on - strand at 14.833 kb on - strand at 14.833 kb on - strand at 14.941 kb on + strand at 14.941 kb on + strand at 14.941 kb on + strand at 14.941 kb on + strand at 14.942 kb on - strand at 14.942 kb on - strand at 14.942 kb on - strand at 14.942 kb on - strand at 16.619 kb on + strand at 16.619 kb on + strand, within Ac3H11_727
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH10 remove 14,171 + -1.7 14,171 + +1.3 14,171 + -0.7 14,171 + -3.1 14,833 - +0.3 14,833 - +0.5 14,833 - -2.6 14,833 - +2.3 14,833 - +1.6 14,941 + -0.3 14,941 + +1.8 14,941 + +0.3 14,941 + -0.3 14,942 - -0.2 14,942 - +0.3 14,942 - -0.9 14,942 - -0.1 16,619 + +1.3 16,619 + Ac3H11_727 0.14 +1.0
Or see this region's nucleotide sequence