Experiment: LB, pH10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_65 and Ac3H11_66 are separated by 247 nucleotides Ac3H11_66 and Ac3H11_67 overlap by 4 nucleotides Ac3H11_67 and Ac3H11_68 are separated by 115 nucleotides
Ac3H11_65 - Chitinase (EC 3.2.1.14), at 67,218 to 68,690
_65
Ac3H11_66 - Benzoate transport, ATPase component, at 68,938 to 69,708
_66
Ac3H11_67 - Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1), at 69,705 to 70,448
_67
Ac3H11_68 - High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1), at 70,564 to 71,550
_68
Position (kb)
68
69
70 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 68.028 kb on + strand, within Ac3H11_65 at 68.046 kb on + strand, within Ac3H11_65 at 68.047 kb on - strand, within Ac3H11_65 at 68.047 kb on - strand, within Ac3H11_65 at 68.086 kb on - strand, within Ac3H11_65 at 68.152 kb on - strand, within Ac3H11_65 at 68.152 kb on - strand, within Ac3H11_65 at 68.331 kb on + strand, within Ac3H11_65 at 68.331 kb on + strand, within Ac3H11_65 at 68.331 kb on + strand, within Ac3H11_65 at 68.331 kb on + strand, within Ac3H11_65 at 68.331 kb on + strand, within Ac3H11_65 at 68.331 kb on + strand, within Ac3H11_65 at 68.332 kb on - strand, within Ac3H11_65 at 68.341 kb on - strand, within Ac3H11_65 at 68.341 kb on - strand, within Ac3H11_65 at 68.388 kb on + strand, within Ac3H11_65 at 68.388 kb on + strand, within Ac3H11_65 at 68.389 kb on - strand, within Ac3H11_65 at 68.421 kb on + strand, within Ac3H11_65 at 68.421 kb on + strand, within Ac3H11_65 at 68.421 kb on + strand, within Ac3H11_65 at 68.422 kb on - strand, within Ac3H11_65 at 68.422 kb on - strand, within Ac3H11_65 at 68.422 kb on - strand, within Ac3H11_65 at 68.422 kb on - strand, within Ac3H11_65 at 68.422 kb on - strand, within Ac3H11_65 at 68.422 kb on - strand, within Ac3H11_65 at 68.422 kb on - strand, within Ac3H11_65 at 68.541 kb on + strand, within Ac3H11_65 at 68.541 kb on + strand, within Ac3H11_65 at 68.541 kb on + strand, within Ac3H11_65 at 68.542 kb on - strand, within Ac3H11_65 at 68.589 kb on + strand at 68.589 kb on + strand at 68.589 kb on + strand at 68.590 kb on - strand at 68.590 kb on - strand at 68.667 kb on + strand at 68.668 kb on - strand at 68.668 kb on - strand at 68.668 kb on - strand at 68.668 kb on - strand at 68.668 kb on - strand at 68.670 kb on + strand at 68.671 kb on - strand at 68.671 kb on - strand at 68.693 kb on + strand at 68.693 kb on + strand at 68.693 kb on + strand at 68.693 kb on + strand at 68.694 kb on - strand at 68.694 kb on - strand at 68.694 kb on - strand at 68.697 kb on + strand at 68.697 kb on + strand at 68.697 kb on + strand at 68.697 kb on + strand at 68.697 kb on + strand at 68.697 kb on + strand at 68.698 kb on - strand at 68.698 kb on - strand at 68.698 kb on - strand at 68.698 kb on - strand at 69.070 kb on + strand, within Ac3H11_66 at 69.070 kb on + strand, within Ac3H11_66 at 69.070 kb on + strand, within Ac3H11_66 at 69.071 kb on - strand, within Ac3H11_66 at 69.071 kb on - strand, within Ac3H11_66 at 69.429 kb on + strand, within Ac3H11_66 at 69.430 kb on - strand, within Ac3H11_66 at 69.430 kb on - strand, within Ac3H11_66 at 69.451 kb on + strand, within Ac3H11_66 at 69.451 kb on + strand, within Ac3H11_66 at 69.451 kb on + strand, within Ac3H11_66 at 69.451 kb on + strand, within Ac3H11_66 at 69.451 kb on + strand, within Ac3H11_66 at 69.452 kb on - strand, within Ac3H11_66 at 69.452 kb on - strand, within Ac3H11_66 at 69.774 kb on + strand at 69.972 kb on + strand, within Ac3H11_67 at 69.973 kb on - strand, within Ac3H11_67 at 70.507 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH10 remove 68,028 + Ac3H11_65 0.55 -0.1 68,046 + Ac3H11_65 0.56 +0.9 68,047 - Ac3H11_65 0.56 +1.4 68,047 - Ac3H11_65 0.56 +0.4 68,086 - Ac3H11_65 0.59 -1.1 68,152 - Ac3H11_65 0.63 -0.8 68,152 - Ac3H11_65 0.63 +1.3 68,331 + Ac3H11_65 0.76 +2.0 68,331 + Ac3H11_65 0.76 +0.8 68,331 + Ac3H11_65 0.76 +0.1 68,331 + Ac3H11_65 0.76 +0.7 68,331 + Ac3H11_65 0.76 +0.4 68,331 + Ac3H11_65 0.76 +0.7 68,332 - Ac3H11_65 0.76 -1.2 68,341 - Ac3H11_65 0.76 -0.8 68,341 - Ac3H11_65 0.76 -1.8 68,388 + Ac3H11_65 0.79 -1.1 68,388 + Ac3H11_65 0.79 -0.3 68,389 - Ac3H11_65 0.79 +0.6 68,421 + Ac3H11_65 0.82 +1.1 68,421 + Ac3H11_65 0.82 -0.5 68,421 + Ac3H11_65 0.82 +0.2 68,422 - Ac3H11_65 0.82 +0.0 68,422 - Ac3H11_65 0.82 -2.4 68,422 - Ac3H11_65 0.82 -0.4 68,422 - Ac3H11_65 0.82 +0.2 68,422 - Ac3H11_65 0.82 -2.4 68,422 - Ac3H11_65 0.82 -0.9 68,422 - Ac3H11_65 0.82 +0.1 68,541 + Ac3H11_65 0.90 +2.6 68,541 + Ac3H11_65 0.90 +1.2 68,541 + Ac3H11_65 0.90 +1.6 68,542 - Ac3H11_65 0.90 -2.8 68,589 + +1.1 68,589 + +0.5 68,589 + -0.8 68,590 - -2.5 68,590 - -2.9 68,667 + +2.2 68,668 - +1.1 68,668 - -2.6 68,668 - -2.9 68,668 - -1.4 68,668 - +0.6 68,670 + +0.4 68,671 - -0.6 68,671 - -0.8 68,693 + +0.4 68,693 + -0.9 68,693 + +2.2 68,693 + +1.3 68,694 - -2.5 68,694 - -0.4 68,694 - +1.3 68,697 + -1.5 68,697 + +0.3 68,697 + +0.6 68,697 + -2.2 68,697 + -0.1 68,697 + +0.1 68,698 - +1.6 68,698 - +0.7 68,698 - -1.6 68,698 - -0.3 69,070 + Ac3H11_66 0.17 +0.9 69,070 + Ac3H11_66 0.17 -2.5 69,070 + Ac3H11_66 0.17 -0.6 69,071 - Ac3H11_66 0.17 +0.5 69,071 - Ac3H11_66 0.17 -1.1 69,429 + Ac3H11_66 0.64 -0.6 69,430 - Ac3H11_66 0.64 -0.6 69,430 - Ac3H11_66 0.64 -1.6 69,451 + Ac3H11_66 0.67 -0.7 69,451 + Ac3H11_66 0.67 -0.1 69,451 + Ac3H11_66 0.67 +1.3 69,451 + Ac3H11_66 0.67 -2.9 69,451 + Ac3H11_66 0.67 +0.6 69,452 - Ac3H11_66 0.67 -1.4 69,452 - Ac3H11_66 0.67 +1.3 69,774 + -0.5 69,972 + Ac3H11_67 0.36 -0.2 69,973 - Ac3H11_67 0.36 +0.5 70,507 - +0.9
Or see this region's nucleotide sequence