Experiment: LB, pH10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_4171 and Ac3H11_4172 overlap by 4 nucleotides Ac3H11_4172 and Ac3H11_4173 overlap by 10 nucleotides
Ac3H11_4171 - D-amino-acid oxidase (EC 1.4.3.3), at 426,841 to 427,956
_4171
Ac3H11_4172 - Hypothetical, similar to sarcosine oxidase alpha subunit, 2Fe-2S domain, at 427,953 to 428,249
_4172
Ac3H11_4173 - Putative oxidoreductase in 4-hydroxyproline catabolic gene cluster, at 428,240 to 429,580
_4173
Position (kb) 427
428
429 Strain fitness (log2 ratio) -2
-1
0
1
2 at 427.537 kb on + strand, within Ac3H11_4171 at 427.538 kb on - strand, within Ac3H11_4171 at 427.538 kb on - strand, within Ac3H11_4171 at 427.699 kb on + strand, within Ac3H11_4171 at 427.700 kb on - strand, within Ac3H11_4171 at 428.010 kb on - strand, within Ac3H11_4172 at 428.030 kb on + strand, within Ac3H11_4172 at 428.030 kb on + strand, within Ac3H11_4172 at 428.030 kb on + strand, within Ac3H11_4172 at 428.030 kb on + strand, within Ac3H11_4172 at 428.031 kb on - strand, within Ac3H11_4172 at 428.031 kb on - strand, within Ac3H11_4172 at 428.031 kb on - strand, within Ac3H11_4172 at 428.880 kb on + strand, within Ac3H11_4173 at 428.880 kb on + strand, within Ac3H11_4173 at 428.880 kb on + strand, within Ac3H11_4173 at 428.884 kb on + strand, within Ac3H11_4173 at 428.884 kb on + strand, within Ac3H11_4173 at 428.884 kb on + strand, within Ac3H11_4173 at 428.884 kb on + strand, within Ac3H11_4173 at 428.885 kb on - strand, within Ac3H11_4173 at 428.907 kb on - strand, within Ac3H11_4173 at 428.976 kb on + strand, within Ac3H11_4173
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH10 remove 427,537 + Ac3H11_4171 0.62 +0.9 427,538 - Ac3H11_4171 0.62 -0.1 427,538 - Ac3H11_4171 0.62 +0.5 427,699 + Ac3H11_4171 0.77 +2.0 427,700 - Ac3H11_4171 0.77 +0.3 428,010 - Ac3H11_4172 0.19 -2.2 428,030 + Ac3H11_4172 0.26 +0.7 428,030 + Ac3H11_4172 0.26 +0.5 428,030 + Ac3H11_4172 0.26 +0.3 428,030 + Ac3H11_4172 0.26 -0.5 428,031 - Ac3H11_4172 0.26 -2.6 428,031 - Ac3H11_4172 0.26 +0.7 428,031 - Ac3H11_4172 0.26 -0.5 428,880 + Ac3H11_4173 0.48 -0.9 428,880 + Ac3H11_4173 0.48 +1.6 428,880 + Ac3H11_4173 0.48 +0.2 428,884 + Ac3H11_4173 0.48 +1.5 428,884 + Ac3H11_4173 0.48 +1.0 428,884 + Ac3H11_4173 0.48 -1.0 428,884 + Ac3H11_4173 0.48 +1.2 428,885 - Ac3H11_4173 0.48 +0.2 428,907 - Ac3H11_4173 0.50 +2.3 428,976 + Ac3H11_4173 0.55 +0.2
Or see this region's nucleotide sequence