Experiment: LB, pH10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_3573 and Ac3H11_3574 are separated by 9 nucleotides Ac3H11_3574 and Ac3H11_3575 are separated by 88 nucleotides Ac3H11_3575 and Ac3H11_3576 are separated by 15 nucleotides Ac3H11_3576 and Ac3H11_3577 overlap by 4 nucleotides Ac3H11_3577 and Ac3H11_3578 overlap by 4 nucleotides
Ac3H11_3573 - ABC-type tungstate transport system, periplasmic binding protein, at 127,918 to 128,757
_3573
Ac3H11_3574 - Molybdenum-binding periplasmic protein, at 128,767 to 129,591
_3574
Ac3H11_3575 - Ubiquinone biosynthesis monooxygenase UbiB, at 129,680 to 131,380
_3575
Ac3H11_3576 - integral membrane protein-like protein, at 131,396 to 131,872
_3576
Ac3H11_3577 - hypothetical protein, at 131,869 to 132,255
_3577
Ac3H11_3578 - Carboxynorspermidine dehydrogenase, at 132,252 to 133,379
_3578
Position (kb) 129
130
131
132 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 129.221 kb on - strand, within Ac3H11_3574 at 129.268 kb on + strand, within Ac3H11_3574 at 129.269 kb on - strand, within Ac3H11_3574 at 129.346 kb on + strand, within Ac3H11_3574 at 129.346 kb on + strand, within Ac3H11_3574 at 129.346 kb on + strand, within Ac3H11_3574 at 129.346 kb on + strand, within Ac3H11_3574 at 129.347 kb on - strand, within Ac3H11_3574 at 129.391 kb on + strand, within Ac3H11_3574 at 129.392 kb on - strand, within Ac3H11_3574 at 129.392 kb on - strand, within Ac3H11_3574 at 129.392 kb on - strand, within Ac3H11_3574 at 129.479 kb on - strand, within Ac3H11_3574 at 130.263 kb on + strand, within Ac3H11_3575 at 130.264 kb on - strand, within Ac3H11_3575 at 130.473 kb on + strand, within Ac3H11_3575 at 130.473 kb on + strand, within Ac3H11_3575 at 130.473 kb on + strand, within Ac3H11_3575 at 130.474 kb on - strand, within Ac3H11_3575 at 131.392 kb on - strand at 131.392 kb on - strand at 131.392 kb on - strand at 131.430 kb on + strand at 131.431 kb on - strand at 131.431 kb on - strand at 131.431 kb on - strand at 131.604 kb on + strand, within Ac3H11_3576 at 131.689 kb on + strand, within Ac3H11_3576 at 131.689 kb on + strand, within Ac3H11_3576 at 131.790 kb on + strand, within Ac3H11_3576 at 131.790 kb on + strand, within Ac3H11_3576 at 131.790 kb on + strand, within Ac3H11_3576 at 131.791 kb on - strand, within Ac3H11_3576 at 131.862 kb on + strand at 131.862 kb on + strand at 131.863 kb on - strand at 132.026 kb on + strand, within Ac3H11_3577 at 132.026 kb on + strand, within Ac3H11_3577 at 132.027 kb on - strand, within Ac3H11_3577
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH10 remove 129,221 - Ac3H11_3574 0.55 +0.1 129,268 + Ac3H11_3574 0.61 -0.5 129,269 - Ac3H11_3574 0.61 -1.2 129,346 + Ac3H11_3574 0.70 -0.2 129,346 + Ac3H11_3574 0.70 -0.3 129,346 + Ac3H11_3574 0.70 -0.5 129,346 + Ac3H11_3574 0.70 +0.9 129,347 - Ac3H11_3574 0.70 +1.2 129,391 + Ac3H11_3574 0.76 +0.6 129,392 - Ac3H11_3574 0.76 +2.1 129,392 - Ac3H11_3574 0.76 -0.3 129,392 - Ac3H11_3574 0.76 -0.8 129,479 - Ac3H11_3574 0.86 -0.0 130,263 + Ac3H11_3575 0.34 -0.4 130,264 - Ac3H11_3575 0.34 -0.5 130,473 + Ac3H11_3575 0.47 -3.1 130,473 + Ac3H11_3575 0.47 +0.5 130,473 + Ac3H11_3575 0.47 -0.4 130,474 - Ac3H11_3575 0.47 +0.3 131,392 - +3.7 131,392 - -1.0 131,392 - +0.2 131,430 + -0.5 131,431 - +1.3 131,431 - +0.3 131,431 - +1.0 131,604 + Ac3H11_3576 0.44 -0.4 131,689 + Ac3H11_3576 0.61 -1.0 131,689 + Ac3H11_3576 0.61 -1.5 131,790 + Ac3H11_3576 0.83 -0.0 131,790 + Ac3H11_3576 0.83 +0.0 131,790 + Ac3H11_3576 0.83 -0.5 131,791 - Ac3H11_3576 0.83 -0.7 131,862 + -1.7 131,862 + -0.5 131,863 - +0.4 132,026 + Ac3H11_3577 0.41 +1.1 132,026 + Ac3H11_3577 0.41 -1.9 132,027 - Ac3H11_3577 0.41 -1.0
Or see this region's nucleotide sequence