Strain Fitness in Acidovorax sp. GW101-3H11 around Ac3H11_3452

Experiment: LB, pH10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAc3H11_3450 and Ac3H11_3451 are separated by 162 nucleotidesAc3H11_3451 and Ac3H11_3452 are separated by 161 nucleotidesAc3H11_3452 and Ac3H11_3648 are separated by 50 nucleotidesAc3H11_3648 and Ac3H11_3453 are separated by 30 nucleotides Ac3H11_3450 - Peptidyl-tRNA hydrolase (EC 3.1.1.29), at 243 to 851 _3450 Ac3H11_3451 - LSU ribosomal protein L25p, at 1,014 to 1,649 _3451 Ac3H11_3452 - Ribose-phosphate pyrophosphokinase (EC 2.7.6.1), at 1,811 to 2,785 _3452 Ac3H11_3648 - tRNA-Gln-TTG, at 2,836 to 2,909 _3648 Ac3H11_3453 - 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (EC 2.7.1.148), at 2,940 to 3,779 _3453 Position (kb) 1 2 3Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1.015 kb on + strandat 1.015 kb on + strandat 1.015 kb on + strandat 1.015 kb on + strandat 1.016 kb on - strandat 1.016 kb on - strandat 1.016 kb on - strandat 1.016 kb on - strandat 1.330 kb on + strand, within Ac3H11_3451at 1.330 kb on + strand, within Ac3H11_3451at 1.330 kb on + strand, within Ac3H11_3451at 1.330 kb on + strand, within Ac3H11_3451at 1.331 kb on - strand, within Ac3H11_3451at 2.830 kb on - strandat 2.830 kb on - strandat 2.830 kb on - strandat 2.830 kb on - strandat 2.830 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB, pH10
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1,015 + -0.6
1,015 + -2.2
1,015 + -3.5
1,015 + -2.9
1,016 - -0.3
1,016 - -1.1
1,016 - +0.1
1,016 - -1.1
1,330 + Ac3H11_3451 0.50 -1.6
1,330 + Ac3H11_3451 0.50 +1.5
1,330 + Ac3H11_3451 0.50 +2.7
1,330 + Ac3H11_3451 0.50 +1.7
1,331 - Ac3H11_3451 0.50 -0.8
2,830 - +1.4
2,830 - -1.7
2,830 - +0.4
2,830 - -2.1
2,830 - -1.0

Or see this region's nucleotide sequence