Experiment: LB, pH10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_340 and Ac3H11_341 are separated by 29 nucleotides Ac3H11_341 and Ac3H11_342 are separated by 90 nucleotides
Ac3H11_340 - 2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34), at 16,444 to 18,510
_340
Ac3H11_341 - FIG00455124: hypothetical protein, at 18,540 to 18,980
_341
Ac3H11_342 - Osmolarity sensory histidine kinase EnvZ, at 19,071 to 20,594
_342
Position (kb) 18
19 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 17.782 kb on + strand, within Ac3H11_340 at 17.783 kb on - strand, within Ac3H11_340 at 17.786 kb on - strand, within Ac3H11_340 at 17.866 kb on + strand, within Ac3H11_340 at 17.894 kb on + strand, within Ac3H11_340 at 17.894 kb on + strand, within Ac3H11_340 at 17.894 kb on + strand, within Ac3H11_340 at 17.894 kb on + strand, within Ac3H11_340 at 17.894 kb on + strand, within Ac3H11_340 at 17.895 kb on - strand, within Ac3H11_340 at 17.947 kb on + strand, within Ac3H11_340 at 17.947 kb on + strand, within Ac3H11_340 at 17.947 kb on + strand, within Ac3H11_340 at 17.948 kb on - strand, within Ac3H11_340 at 18.276 kb on - strand, within Ac3H11_340 at 18.280 kb on + strand, within Ac3H11_340 at 18.280 kb on + strand, within Ac3H11_340 at 18.281 kb on - strand, within Ac3H11_340 at 18.391 kb on + strand at 18.391 kb on + strand at 18.392 kb on - strand at 18.392 kb on - strand at 18.392 kb on - strand at 18.687 kb on + strand, within Ac3H11_341 at 18.688 kb on - strand, within Ac3H11_341 at 18.688 kb on - strand, within Ac3H11_341 at 18.688 kb on - strand, within Ac3H11_341 at 18.753 kb on + strand, within Ac3H11_341 at 18.753 kb on + strand, within Ac3H11_341 at 18.753 kb on + strand, within Ac3H11_341 at 18.753 kb on + strand, within Ac3H11_341 at 18.754 kb on - strand, within Ac3H11_341 at 18.754 kb on - strand, within Ac3H11_341 at 18.754 kb on - strand, within Ac3H11_341 at 18.950 kb on + strand at 18.950 kb on + strand at 18.951 kb on - strand at 18.951 kb on - strand at 18.951 kb on - strand at 19.072 kb on + strand at 19.072 kb on + strand at 19.073 kb on - strand at 19.073 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH10 remove 17,782 + Ac3H11_340 0.65 -3.6 17,783 - Ac3H11_340 0.65 +0.5 17,786 - Ac3H11_340 0.65 +0.9 17,866 + Ac3H11_340 0.69 +0.8 17,894 + Ac3H11_340 0.70 +1.8 17,894 + Ac3H11_340 0.70 -1.6 17,894 + Ac3H11_340 0.70 -2.1 17,894 + Ac3H11_340 0.70 +0.1 17,894 + Ac3H11_340 0.70 -1.0 17,895 - Ac3H11_340 0.70 -0.4 17,947 + Ac3H11_340 0.73 +1.7 17,947 + Ac3H11_340 0.73 +0.9 17,947 + Ac3H11_340 0.73 -0.7 17,948 - Ac3H11_340 0.73 +0.4 18,276 - Ac3H11_340 0.89 +0.2 18,280 + Ac3H11_340 0.89 -2.1 18,280 + Ac3H11_340 0.89 -0.0 18,281 - Ac3H11_340 0.89 -2.5 18,391 + +1.5 18,391 + +1.2 18,392 - -0.5 18,392 - -0.9 18,392 - -0.2 18,687 + Ac3H11_341 0.33 -2.1 18,688 - Ac3H11_341 0.34 -2.1 18,688 - Ac3H11_341 0.34 -1.4 18,688 - Ac3H11_341 0.34 +0.2 18,753 + Ac3H11_341 0.48 -0.2 18,753 + Ac3H11_341 0.48 -1.5 18,753 + Ac3H11_341 0.48 +0.2 18,753 + Ac3H11_341 0.48 -0.6 18,754 - Ac3H11_341 0.49 +0.3 18,754 - Ac3H11_341 0.49 +0.7 18,754 - Ac3H11_341 0.49 +0.2 18,950 + +1.7 18,950 + +0.8 18,951 - -2.6 18,951 - +1.0 18,951 - -1.4 19,072 + +1.8 19,072 + +1.8 19,073 - +1.0 19,073 - +1.5
Or see this region's nucleotide sequence