Strain Fitness in Acidovorax sp. GW101-3H11 around Ac3H11_2549

Experiment: LB, pH10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAc3H11_2547 and Ac3H11_2548 are separated by 66 nucleotidesAc3H11_2548 and Ac3H11_2549 are separated by 36 nucleotidesAc3H11_2549 and Ac3H11_2550 overlap by 4 nucleotides Ac3H11_2547 - Phosphatidylserine decarboxylase (EC 4.1.1.65), at 824,742 to 825,590 _2547 Ac3H11_2548 - N-formylglutamate deformylase (EC 3.5.1.68) (from data), at 825,657 to 826,469 _2548 Ac3H11_2549 - Formiminoglutamic iminohydrolase (EC 3.5.3.13) (from data), at 826,506 to 827,900 _2549 Ac3H11_2550 - Imidazolonepropionase (EC 3.5.2.7) (from data), at 827,897 to 829,186 _2550 Position (kb) 826 827 828Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 825.732 kb on + strandat 825.733 kb on - strandat 825.746 kb on - strand, within Ac3H11_2548at 825.746 kb on - strand, within Ac3H11_2548at 825.770 kb on + strand, within Ac3H11_2548at 825.770 kb on + strand, within Ac3H11_2548at 825.771 kb on - strand, within Ac3H11_2548at 825.771 kb on - strand, within Ac3H11_2548at 825.771 kb on - strand, within Ac3H11_2548at 825.771 kb on - strand, within Ac3H11_2548at 825.771 kb on - strand, within Ac3H11_2548at 825.772 kb on + strand, within Ac3H11_2548at 825.772 kb on + strand, within Ac3H11_2548at 825.772 kb on + strand, within Ac3H11_2548at 825.772 kb on + strand, within Ac3H11_2548at 825.773 kb on - strand, within Ac3H11_2548at 825.773 kb on - strand, within Ac3H11_2548at 825.773 kb on - strand, within Ac3H11_2548at 825.773 kb on - strand, within Ac3H11_2548at 825.773 kb on - strand, within Ac3H11_2548at 825.773 kb on - strand, within Ac3H11_2548at 825.773 kb on - strand, within Ac3H11_2548at 825.773 kb on - strand, within Ac3H11_2548at 825.773 kb on - strand, within Ac3H11_2548at 825.773 kb on - strand, within Ac3H11_2548at 826.087 kb on + strand, within Ac3H11_2548at 826.088 kb on - strand, within Ac3H11_2548at 826.153 kb on + strand, within Ac3H11_2548at 826.153 kb on + strand, within Ac3H11_2548at 826.154 kb on - strand, within Ac3H11_2548at 826.154 kb on - strand, within Ac3H11_2548at 826.175 kb on + strand, within Ac3H11_2548at 826.213 kb on + strand, within Ac3H11_2548at 826.213 kb on + strand, within Ac3H11_2548at 826.256 kb on - strand, within Ac3H11_2548at 826.256 kb on - strand, within Ac3H11_2548at 826.309 kb on + strand, within Ac3H11_2548at 826.309 kb on + strand, within Ac3H11_2548at 826.309 kb on + strand, within Ac3H11_2548at 826.310 kb on - strand, within Ac3H11_2548at 826.310 kb on - strand, within Ac3H11_2548at 826.310 kb on - strand, within Ac3H11_2548at 826.310 kb on - strand, within Ac3H11_2548at 826.390 kb on + strandat 826.390 kb on + strandat 826.390 kb on + strandat 826.390 kb on + strandat 826.390 kb on + strandat 826.390 kb on + strandat 826.390 kb on + strandat 826.390 kb on + strandat 826.390 kb on + strandat 826.390 kb on + strandat 826.390 kb on + strandat 826.390 kb on + strandat 826.390 kb on + strandat 826.390 kb on + strandat 826.390 kb on + strandat 826.391 kb on - strandat 826.391 kb on - strandat 826.391 kb on - strandat 826.391 kb on - strandat 826.391 kb on - strandat 826.469 kb on + strandat 826.470 kb on - strandat 826.470 kb on - strandat 826.470 kb on - strandat 826.539 kb on + strandat 826.871 kb on - strand, within Ac3H11_2549at 827.045 kb on - strand, within Ac3H11_2549at 827.216 kb on + strand, within Ac3H11_2549at 827.217 kb on - strand, within Ac3H11_2549at 827.217 kb on - strand, within Ac3H11_2549at 827.410 kb on + strand, within Ac3H11_2549at 827.411 kb on - strand, within Ac3H11_2549at 827.494 kb on + strand, within Ac3H11_2549at 827.524 kb on + strand, within Ac3H11_2549at 827.549 kb on - strand, within Ac3H11_2549at 827.572 kb on + strand, within Ac3H11_2549at 827.572 kb on + strand, within Ac3H11_2549at 827.572 kb on + strand, within Ac3H11_2549at 827.572 kb on + strand, within Ac3H11_2549at 827.572 kb on + strand, within Ac3H11_2549at 827.572 kb on + strand, within Ac3H11_2549at 827.573 kb on - strand, within Ac3H11_2549at 827.964 kb on + strandat 828.073 kb on + strand, within Ac3H11_2550at 828.182 kb on - strand, within Ac3H11_2550at 828.252 kb on + strand, within Ac3H11_2550at 828.252 kb on + strand, within Ac3H11_2550at 828.256 kb on - strand, within Ac3H11_2550at 828.354 kb on + strand, within Ac3H11_2550at 828.559 kb on - strand, within Ac3H11_2550at 828.559 kb on - strand, within Ac3H11_2550at 828.559 kb on - strand, within Ac3H11_2550at 828.635 kb on + strand, within Ac3H11_2550at 828.635 kb on + strand, within Ac3H11_2550at 828.636 kb on - strand, within Ac3H11_2550at 828.636 kb on - strand, within Ac3H11_2550at 828.846 kb on + strand, within Ac3H11_2550at 828.897 kb on + strand, within Ac3H11_2550

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Per-strain Table

Position Strand Gene LocusTag Fraction LB, pH10
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825,732 + +0.3
825,733 - -0.4
825,746 - Ac3H11_2548 0.11 -1.1
825,746 - Ac3H11_2548 0.11 -0.6
825,770 + Ac3H11_2548 0.14 -2.2
825,770 + Ac3H11_2548 0.14 +0.6
825,771 - Ac3H11_2548 0.14 +0.4
825,771 - Ac3H11_2548 0.14 +1.5
825,771 - Ac3H11_2548 0.14 +0.4
825,771 - Ac3H11_2548 0.14 -0.6
825,771 - Ac3H11_2548 0.14 -2.3
825,772 + Ac3H11_2548 0.14 -0.4
825,772 + Ac3H11_2548 0.14 -1.7
825,772 + Ac3H11_2548 0.14 -0.2
825,772 + Ac3H11_2548 0.14 +2.6
825,773 - Ac3H11_2548 0.14 -1.6
825,773 - Ac3H11_2548 0.14 +0.5
825,773 - Ac3H11_2548 0.14 -1.1
825,773 - Ac3H11_2548 0.14 +0.4
825,773 - Ac3H11_2548 0.14 -1.3
825,773 - Ac3H11_2548 0.14 -0.7
825,773 - Ac3H11_2548 0.14 -2.2
825,773 - Ac3H11_2548 0.14 +0.0
825,773 - Ac3H11_2548 0.14 -0.1
825,773 - Ac3H11_2548 0.14 +0.6
826,087 + Ac3H11_2548 0.53 -1.8
826,088 - Ac3H11_2548 0.53 +0.5
826,153 + Ac3H11_2548 0.61 +2.1
826,153 + Ac3H11_2548 0.61 -0.1
826,154 - Ac3H11_2548 0.61 +0.8
826,154 - Ac3H11_2548 0.61 -0.4
826,175 + Ac3H11_2548 0.64 -0.4
826,213 + Ac3H11_2548 0.68 +0.0
826,213 + Ac3H11_2548 0.68 -1.8
826,256 - Ac3H11_2548 0.74 -0.3
826,256 - Ac3H11_2548 0.74 -1.4
826,309 + Ac3H11_2548 0.80 +0.4
826,309 + Ac3H11_2548 0.80 +0.8
826,309 + Ac3H11_2548 0.80 +0.4
826,310 - Ac3H11_2548 0.80 +2.2
826,310 - Ac3H11_2548 0.80 +1.4
826,310 - Ac3H11_2548 0.80 -0.5
826,310 - Ac3H11_2548 0.80 -0.3
826,390 + +2.3
826,390 + -1.5
826,390 + +0.5
826,390 + -0.4
826,390 + -0.9
826,390 + -1.4
826,390 + +0.9
826,390 + -0.6
826,390 + +0.8
826,390 + -0.2
826,390 + -0.8
826,390 + +1.5
826,390 + -2.1
826,390 + +0.7
826,390 + -1.0
826,391 - -2.6
826,391 - -0.5
826,391 - -0.1
826,391 - -0.6
826,391 - -0.0
826,469 + -2.2
826,470 - +1.1
826,470 - -1.9
826,470 - +0.0
826,539 + -0.2
826,871 - Ac3H11_2549 0.26 -1.5
827,045 - Ac3H11_2549 0.39 -0.7
827,216 + Ac3H11_2549 0.51 -1.2
827,217 - Ac3H11_2549 0.51 -1.9
827,217 - Ac3H11_2549 0.51 -1.0
827,410 + Ac3H11_2549 0.65 -1.8
827,411 - Ac3H11_2549 0.65 -1.4
827,494 + Ac3H11_2549 0.71 -1.9
827,524 + Ac3H11_2549 0.73 -1.4
827,549 - Ac3H11_2549 0.75 -1.3
827,572 + Ac3H11_2549 0.76 -1.6
827,572 + Ac3H11_2549 0.76 -1.7
827,572 + Ac3H11_2549 0.76 -1.1
827,572 + Ac3H11_2549 0.76 -1.2
827,572 + Ac3H11_2549 0.76 -1.1
827,572 + Ac3H11_2549 0.76 -1.8
827,573 - Ac3H11_2549 0.76 -2.2
827,964 + +1.0
828,073 + Ac3H11_2550 0.14 -2.2
828,182 - Ac3H11_2550 0.22 +0.0
828,252 + Ac3H11_2550 0.28 +0.1
828,252 + Ac3H11_2550 0.28 +0.5
828,256 - Ac3H11_2550 0.28 +3.4
828,354 + Ac3H11_2550 0.35 -2.8
828,559 - Ac3H11_2550 0.51 -0.5
828,559 - Ac3H11_2550 0.51 -0.0
828,559 - Ac3H11_2550 0.51 -2.0
828,635 + Ac3H11_2550 0.57 -1.6
828,635 + Ac3H11_2550 0.57 +0.5
828,636 - Ac3H11_2550 0.57 -0.2
828,636 - Ac3H11_2550 0.57 -1.5
828,846 + Ac3H11_2550 0.74 +1.2
828,897 + Ac3H11_2550 0.78 -0.5

Or see this region's nucleotide sequence