Experiment: LB, pH10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1299 and Ac3H11_1300 are separated by 70 nucleotides Ac3H11_1300 and Ac3H11_1301 are separated by 265 nucleotides Ac3H11_1301 and Ac3H11_1302 are separated by 223 nucleotides
Ac3H11_1299 - PhnB protein; putative DNA binding 3-demethylubiquinone-9 3-methyltransferase domain protein, at 114,339 to 114,767
_1299
Ac3H11_1300 - hypothetical protein, at 114,838 to 115,323
_1300
Ac3H11_1301 - MFS family multidrug efflux protein in Burkholderiaceae, unknown substrate, at 115,589 to 117,070
_1301
Ac3H11_1302 - Pirin, N-terminal:Pirin, C-terminal, at 117,294 to 118,391
_1302
Position (kb)
115
116
117
118 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 115.120 kb on + strand, within Ac3H11_1300 at 115.121 kb on - strand, within Ac3H11_1300 at 115.306 kb on + strand at 115.306 kb on + strand at 115.307 kb on - strand at 115.307 kb on - strand at 115.307 kb on - strand at 115.397 kb on + strand at 115.426 kb on + strand at 115.426 kb on + strand at 115.427 kb on - strand at 115.796 kb on + strand, within Ac3H11_1301 at 115.796 kb on + strand, within Ac3H11_1301 at 115.796 kb on + strand, within Ac3H11_1301 at 115.796 kb on + strand, within Ac3H11_1301 at 115.796 kb on + strand, within Ac3H11_1301 at 115.796 kb on + strand, within Ac3H11_1301 at 115.796 kb on + strand, within Ac3H11_1301 at 115.796 kb on + strand, within Ac3H11_1301 at 115.797 kb on - strand, within Ac3H11_1301 at 115.797 kb on - strand, within Ac3H11_1301 at 115.797 kb on - strand, within Ac3H11_1301 at 115.797 kb on - strand, within Ac3H11_1301 at 115.797 kb on - strand, within Ac3H11_1301 at 115.797 kb on - strand, within Ac3H11_1301 at 115.797 kb on - strand, within Ac3H11_1301 at 115.797 kb on - strand, within Ac3H11_1301 at 115.850 kb on + strand, within Ac3H11_1301 at 115.850 kb on + strand, within Ac3H11_1301 at 115.851 kb on - strand, within Ac3H11_1301 at 116.054 kb on + strand, within Ac3H11_1301 at 116.054 kb on + strand, within Ac3H11_1301 at 116.054 kb on + strand, within Ac3H11_1301 at 116.055 kb on - strand, within Ac3H11_1301 at 116.055 kb on - strand, within Ac3H11_1301 at 116.055 kb on - strand, within Ac3H11_1301 at 116.055 kb on - strand, within Ac3H11_1301 at 116.055 kb on - strand, within Ac3H11_1301 at 116.171 kb on + strand, within Ac3H11_1301 at 116.171 kb on + strand, within Ac3H11_1301 at 116.172 kb on - strand, within Ac3H11_1301 at 116.279 kb on + strand, within Ac3H11_1301 at 116.279 kb on + strand, within Ac3H11_1301 at 116.279 kb on + strand, within Ac3H11_1301 at 116.279 kb on + strand, within Ac3H11_1301 at 116.280 kb on - strand, within Ac3H11_1301 at 116.280 kb on - strand, within Ac3H11_1301 at 116.280 kb on - strand, within Ac3H11_1301 at 116.280 kb on - strand, within Ac3H11_1301 at 116.280 kb on - strand, within Ac3H11_1301 at 116.280 kb on - strand, within Ac3H11_1301 at 116.504 kb on + strand, within Ac3H11_1301 at 116.504 kb on + strand, within Ac3H11_1301 at 116.504 kb on + strand, within Ac3H11_1301 at 116.505 kb on - strand, within Ac3H11_1301 at 116.505 kb on - strand, within Ac3H11_1301 at 116.505 kb on - strand, within Ac3H11_1301 at 116.505 kb on - strand, within Ac3H11_1301 at 116.525 kb on + strand, within Ac3H11_1301 at 116.765 kb on - strand, within Ac3H11_1301 at 116.796 kb on + strand, within Ac3H11_1301 at 116.796 kb on + strand, within Ac3H11_1301 at 116.796 kb on + strand, within Ac3H11_1301 at 116.797 kb on - strand, within Ac3H11_1301 at 117.085 kb on + strand at 117.086 kb on - strand at 117.241 kb on - strand at 117.504 kb on + strand, within Ac3H11_1302 at 117.504 kb on + strand, within Ac3H11_1302 at 117.504 kb on + strand, within Ac3H11_1302 at 117.505 kb on - strand, within Ac3H11_1302 at 118.035 kb on + strand, within Ac3H11_1302 at 118.035 kb on + strand, within Ac3H11_1302 at 118.035 kb on + strand, within Ac3H11_1302 at 118.035 kb on + strand, within Ac3H11_1302 at 118.035 kb on + strand, within Ac3H11_1302 at 118.036 kb on - strand, within Ac3H11_1302
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH10 remove 115,120 + Ac3H11_1300 0.58 +1.8 115,121 - Ac3H11_1300 0.58 +1.1 115,306 + -1.0 115,306 + +0.1 115,307 - +0.6 115,307 - +1.2 115,307 - -0.4 115,397 + -0.6 115,426 + -1.2 115,426 + +0.4 115,427 - -0.5 115,796 + Ac3H11_1301 0.14 -0.7 115,796 + Ac3H11_1301 0.14 -1.3 115,796 + Ac3H11_1301 0.14 +0.4 115,796 + Ac3H11_1301 0.14 -1.2 115,796 + Ac3H11_1301 0.14 -0.2 115,796 + Ac3H11_1301 0.14 -0.0 115,796 + Ac3H11_1301 0.14 +1.4 115,796 + Ac3H11_1301 0.14 +1.1 115,797 - Ac3H11_1301 0.14 +1.2 115,797 - Ac3H11_1301 0.14 +0.8 115,797 - Ac3H11_1301 0.14 -1.0 115,797 - Ac3H11_1301 0.14 +0.1 115,797 - Ac3H11_1301 0.14 +0.3 115,797 - Ac3H11_1301 0.14 -0.0 115,797 - Ac3H11_1301 0.14 +0.0 115,797 - Ac3H11_1301 0.14 +0.7 115,850 + Ac3H11_1301 0.18 -1.9 115,850 + Ac3H11_1301 0.18 +1.6 115,851 - Ac3H11_1301 0.18 +1.3 116,054 + Ac3H11_1301 0.31 +0.2 116,054 + Ac3H11_1301 0.31 +0.4 116,054 + Ac3H11_1301 0.31 +1.3 116,055 - Ac3H11_1301 0.31 -2.2 116,055 - Ac3H11_1301 0.31 +1.3 116,055 - Ac3H11_1301 0.31 +3.0 116,055 - Ac3H11_1301 0.31 +0.7 116,055 - Ac3H11_1301 0.31 +2.4 116,171 + Ac3H11_1301 0.39 -1.8 116,171 + Ac3H11_1301 0.39 -1.0 116,172 - Ac3H11_1301 0.39 -2.2 116,279 + Ac3H11_1301 0.47 -2.2 116,279 + Ac3H11_1301 0.47 -0.7 116,279 + Ac3H11_1301 0.47 -0.6 116,279 + Ac3H11_1301 0.47 +3.9 116,280 - Ac3H11_1301 0.47 +1.0 116,280 - Ac3H11_1301 0.47 +1.6 116,280 - Ac3H11_1301 0.47 -0.7 116,280 - Ac3H11_1301 0.47 +2.0 116,280 - Ac3H11_1301 0.47 -0.1 116,280 - Ac3H11_1301 0.47 +1.5 116,504 + Ac3H11_1301 0.62 +0.3 116,504 + Ac3H11_1301 0.62 +0.2 116,504 + Ac3H11_1301 0.62 +2.3 116,505 - Ac3H11_1301 0.62 +0.8 116,505 - Ac3H11_1301 0.62 +1.8 116,505 - Ac3H11_1301 0.62 +1.1 116,505 - Ac3H11_1301 0.62 -1.0 116,525 + Ac3H11_1301 0.63 +1.4 116,765 - Ac3H11_1301 0.79 +0.8 116,796 + Ac3H11_1301 0.81 +0.3 116,796 + Ac3H11_1301 0.81 -0.6 116,796 + Ac3H11_1301 0.81 +0.6 116,797 - Ac3H11_1301 0.82 +1.9 117,085 + +1.2 117,086 - +0.4 117,241 - -0.0 117,504 + Ac3H11_1302 0.19 -0.3 117,504 + Ac3H11_1302 0.19 +0.6 117,504 + Ac3H11_1302 0.19 -0.0 117,505 - Ac3H11_1302 0.19 +0.1 118,035 + Ac3H11_1302 0.67 -0.1 118,035 + Ac3H11_1302 0.67 -2.0 118,035 + Ac3H11_1302 0.67 +0.9 118,035 + Ac3H11_1302 0.67 -0.9 118,035 + Ac3H11_1302 0.67 -2.3 118,036 - Ac3H11_1302 0.68 +1.9
Or see this region's nucleotide sequence