Experiment: LB, pH10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ac3H11_1035 and Ac3H11_1036 are separated by 30 nucleotides Ac3H11_1036 and Ac3H11_1037 overlap by 4 nucleotides Ac3H11_1037 and Ac3H11_1038 are separated by 167 nucleotides
Ac3H11_1035 - Transcriptional regulator, AraC family, at 153,245 to 154,267
_1035
Ac3H11_1036 - Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16), at 154,298 to 156,409
_1036
Ac3H11_1037 - Isoquinoline 1-oxidoreductase alpha subunit (EC 1.3.99.16), at 156,406 to 156,870
_1037
Ac3H11_1038 - suppressor for copper-sensitivity D, putative, at 157,038 to 157,625
_1038
Position (kb) 154
155
156
157 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 153.975 kb on + strand, within Ac3H11_1035 at 153.975 kb on + strand, within Ac3H11_1035 at 153.975 kb on + strand, within Ac3H11_1035 at 153.975 kb on + strand, within Ac3H11_1035 at 153.976 kb on - strand, within Ac3H11_1035 at 153.976 kb on - strand, within Ac3H11_1035 at 153.976 kb on - strand, within Ac3H11_1035 at 153.976 kb on - strand, within Ac3H11_1035 at 153.976 kb on - strand, within Ac3H11_1035 at 154.140 kb on + strand, within Ac3H11_1035 at 154.140 kb on + strand, within Ac3H11_1035 at 154.141 kb on - strand, within Ac3H11_1035 at 154.141 kb on - strand, within Ac3H11_1035 at 154.653 kb on - strand, within Ac3H11_1036 at 154.653 kb on - strand, within Ac3H11_1036 at 154.653 kb on - strand, within Ac3H11_1036 at 154.717 kb on - strand, within Ac3H11_1036 at 154.717 kb on - strand, within Ac3H11_1036 at 154.717 kb on - strand, within Ac3H11_1036 at 154.732 kb on - strand, within Ac3H11_1036 at 154.732 kb on - strand, within Ac3H11_1036 at 154.732 kb on - strand, within Ac3H11_1036 at 154.732 kb on - strand, within Ac3H11_1036 at 155.391 kb on + strand, within Ac3H11_1036 at 155.391 kb on + strand, within Ac3H11_1036 at 155.392 kb on - strand, within Ac3H11_1036 at 155.392 kb on - strand, within Ac3H11_1036 at 155.392 kb on - strand, within Ac3H11_1036 at 155.392 kb on - strand, within Ac3H11_1036 at 155.392 kb on - strand, within Ac3H11_1036 at 155.392 kb on - strand, within Ac3H11_1036 at 155.392 kb on - strand, within Ac3H11_1036 at 155.392 kb on - strand, within Ac3H11_1036 at 155.417 kb on + strand, within Ac3H11_1036 at 155.417 kb on + strand, within Ac3H11_1036 at 155.418 kb on - strand, within Ac3H11_1036 at 155.418 kb on - strand, within Ac3H11_1036 at 155.418 kb on - strand, within Ac3H11_1036 at 155.418 kb on - strand, within Ac3H11_1036 at 155.865 kb on + strand, within Ac3H11_1036 at 156.179 kb on + strand, within Ac3H11_1036 at 156.179 kb on + strand, within Ac3H11_1036 at 156.179 kb on + strand, within Ac3H11_1036 at 156.179 kb on + strand, within Ac3H11_1036 at 156.180 kb on - strand, within Ac3H11_1036 at 156.261 kb on + strand at 156.261 kb on + strand at 156.262 kb on - strand at 156.262 kb on - strand at 156.282 kb on - strand at 157.035 kb on + strand at 157.035 kb on + strand at 157.039 kb on + strand at 157.120 kb on + strand, within Ac3H11_1038 at 157.222 kb on - strand, within Ac3H11_1038 at 157.316 kb on + strand, within Ac3H11_1038 at 157.316 kb on + strand, within Ac3H11_1038 at 157.317 kb on - strand, within Ac3H11_1038 at 157.317 kb on - strand, within Ac3H11_1038 at 157.317 kb on - strand, within Ac3H11_1038
Per-strain Table
Position Strand Gene LocusTag Fraction LB, pH10 remove 153,975 + Ac3H11_1035 0.71 +0.1 153,975 + Ac3H11_1035 0.71 +2.3 153,975 + Ac3H11_1035 0.71 -1.8 153,975 + Ac3H11_1035 0.71 +0.4 153,976 - Ac3H11_1035 0.71 -1.8 153,976 - Ac3H11_1035 0.71 -1.6 153,976 - Ac3H11_1035 0.71 -1.1 153,976 - Ac3H11_1035 0.71 -2.2 153,976 - Ac3H11_1035 0.71 -0.8 154,140 + Ac3H11_1035 0.87 -0.5 154,140 + Ac3H11_1035 0.87 +1.0 154,141 - Ac3H11_1035 0.88 +1.2 154,141 - Ac3H11_1035 0.88 -0.8 154,653 - Ac3H11_1036 0.17 +0.7 154,653 - Ac3H11_1036 0.17 -0.3 154,653 - Ac3H11_1036 0.17 +0.5 154,717 - Ac3H11_1036 0.20 -1.6 154,717 - Ac3H11_1036 0.20 -1.1 154,717 - Ac3H11_1036 0.20 +1.1 154,732 - Ac3H11_1036 0.21 +0.1 154,732 - Ac3H11_1036 0.21 -1.6 154,732 - Ac3H11_1036 0.21 +1.1 154,732 - Ac3H11_1036 0.21 +0.6 155,391 + Ac3H11_1036 0.52 +3.3 155,391 + Ac3H11_1036 0.52 -1.6 155,392 - Ac3H11_1036 0.52 +0.6 155,392 - Ac3H11_1036 0.52 +1.0 155,392 - Ac3H11_1036 0.52 +1.4 155,392 - Ac3H11_1036 0.52 -0.4 155,392 - Ac3H11_1036 0.52 -0.3 155,392 - Ac3H11_1036 0.52 -0.8 155,392 - Ac3H11_1036 0.52 -1.4 155,392 - Ac3H11_1036 0.52 -1.6 155,417 + Ac3H11_1036 0.53 -2.6 155,417 + Ac3H11_1036 0.53 +0.4 155,418 - Ac3H11_1036 0.53 +0.3 155,418 - Ac3H11_1036 0.53 -0.7 155,418 - Ac3H11_1036 0.53 -1.7 155,418 - Ac3H11_1036 0.53 +3.6 155,865 + Ac3H11_1036 0.74 -2.3 156,179 + Ac3H11_1036 0.89 +0.0 156,179 + Ac3H11_1036 0.89 +2.5 156,179 + Ac3H11_1036 0.89 -0.1 156,179 + Ac3H11_1036 0.89 -0.1 156,180 - Ac3H11_1036 0.89 -0.8 156,261 + -2.1 156,261 + +1.3 156,262 - +1.2 156,262 - -0.2 156,282 - +1.2 157,035 + -1.6 157,035 + -4.0 157,039 + -0.2 157,120 + Ac3H11_1038 0.14 +1.4 157,222 - Ac3H11_1038 0.31 +1.2 157,316 + Ac3H11_1038 0.47 +1.2 157,316 + Ac3H11_1038 0.47 -0.0 157,317 - Ac3H11_1038 0.47 +1.1 157,317 - Ac3H11_1038 0.47 +0.9 157,317 - Ac3H11_1038 0.47 +1.0
Or see this region's nucleotide sequence